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The expression of cytokines by an established basal cell carcinoma cell line (BCC-1/KMC) compared with cultured normal keratinocytes

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Abstract

A basal cell carcinoma (BCC) cell line (BCC-1/KMC) has recently been successfully established from a patient. The production of interleukin-1α (IL-1α), IL-1β, IL-6 and IL-8 was assessed in comparison with that of cultured normal keratinocytes. The mRNA expression of these cytokines was measured by a reverse transcriptase-polymerase chain reaction (RT-PCR) method and the protein production by an ELISA. The cultured BCC cells spontaneously secreted more IL-6 and IL-8 but less IL-1 than the keratinocytes after culture for 24 h at 37°C. It is suggested that the increased expression of IL-6 and IL-8 may indicate the transformation of normal keratinocytes to locally aggressive BCC.

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Authors and Affiliations

  1. Graduate Institute of Medical Sciences, Kaohsiung Medical College, 100 Shih Chuan 1st Road, 80731, Kaohsiung, Taiwan, ROC

    Huai-Tzu Yen & Kou-Houng Wen

  2. Department of Microbiology, Kaohsiung Medical College, 100 Shih Chuan 1st Road, 80731, Kaohsiung, Taiwan, ROC

    Lien-Chai Chiang

  3. Graduate Institute of Dental Sciences, Kaohsiung Medical College, 100 Shih Chuan 1st Road, 80731, Kaohsiung, Taiwan, ROC

    Chi-Cheng Tsai

  4. Section of Allergy, Immunology and Rheumatology, Veterans General Hospital Taipei, National Yang-Ming University School of Medicine, 11217, Taipei, ROC

    Chia-Li Yu

  5. Department of Dermatology, Kaohsiung Medical College, 100 Shih Chuan 1st Road, 80731, Kaohsiung, Taiwan, ROC

    Hsin-Su Yu

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Yen, HT., Chiang, LC., Wen, KH. et al. The expression of cytokines by an established basal cell carcinoma cell line (BCC-1/KMC) compared with cultured normal keratinocytes. Arch Dermatol Res 288, 157–161 (1996). https://doi.org/10.1007/BF02505826

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  • DOI: https://doi.org/10.1007/BF02505826

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