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Analysis of Cytokines and ATP in Plucked Hair Follicles

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The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length <1 mm and ATP content surpassed the normal in follicles >2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.

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Authors and Affiliations

  1. Federal Research Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia

    E. V. Mikhalchik & O. V. Morozova

  2. Moscow Practical Center of Dermatovenerology and Cosmetology, Moscow Healthcare Department, Moscow, Russia

    T. V. Tsimbalenko & A. G. Gadzhigoroeva

  3. H. M. Berbekov Kabardino-Balkarian State University, Nalchik, Kabardino-Balkarian Republic, Russia

    Z. F. Kharaeva

  4. Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, Russia

    N. G. Balabushevich

  5. N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia

    V. A. Lipatova

Authors
  1. E. V. Mikhalchik
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  2. O. V. Morozova
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  3. T. V. Tsimbalenko
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  4. Z. F. Kharaeva
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  5. N. G. Balabushevich
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  6. V. A. Lipatova
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  7. A. G. Gadzhigoroeva
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Corresponding author

Correspondence to E. V. Mikhalchik.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 9, pp. 286-289, September, 2020

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Mikhalchik, E.V., Morozova, O.V., Tsimbalenko, T.V. et al. Analysis of Cytokines and ATP in Plucked Hair Follicles. Bull Exp Biol Med 170, 299–302 (2021). https://doi.org/10.1007/s10517-021-05055-0

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  • DOI: https://doi.org/10.1007/s10517-021-05055-0

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