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Synthesis and characterization of a chemically-bonded cholesteric stationary phase for high-performance liquid chromatogrphy

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Summary

A bonded, cholesteric stationary phase for high performance liquid chromatography was prepared by reacting the cholesteryl chloroformate with the terminal amino group of a bonded aminopropyl silica from Merck. Infrared Fourier transform spectrometry; carbon-13 cross polarization magic angle spinning NMR and differential scanning calorimetry confirmed the success of the bonding reaction. Carbon elemental analysis indicated a high loading of the cholesteric moiety on the surface and the argon adsorption method was used to measure the specific surface of this new bonded stationary phase. Van't Hoff plots of both cholesteric phase and RP-18 confirm the different behaviour. Examples of selectivities are given.

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Authors and Affiliations

  1. UMR Synthèse et Catalyse, Faculté des Sciences et Techniques de St Jérome, Université d'Axis-Marseille III, 13397, Marseille cedex 20, France

    C. Delaurent, V. Tomao & A. M. Siouffi

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  1. C. Delaurent
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  2. V. Tomao
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  3. A. M. Siouffi
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Delaurent, C., Tomao, V. & Siouffi, A.M. Synthesis and characterization of a chemically-bonded cholesteric stationary phase for high-performance liquid chromatogrphy. Chromatographia 45, 355–363 (1997). https://doi.org/10.1007/BF02505584

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  • DOI: https://doi.org/10.1007/BF02505584

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