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Analysis of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in plasma

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Summary

A method for the determination of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in plasma was developed. 5-HNMP and 2-HMSI are metabolites to the widely used organic solvent N-methyl-2pyrrolidone (NMP). The 5-HNMP and 2-HMSI were purified from plasma by C8 solid phase extraction, derivatised by bistrimethylsilyl trifluoroacetamid, and analysed by gas chromatography with mass spectrometric detection. For 5-HNMP, the precision was 2–7 % (120 and 780 ng mL−1) and the detection limit was 6 ng mL−1 (m/z 98). For 2-HMSI, the precision was 2–9 % (160 and 1000 ng mL−1) and the detection limit was 4 ng mL−1 (m/z 144). The method is applicable for analysis of plasma samples from workers exposed to NMP.

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References

  1. B. Åkesson, Arbete Halsa40, 1 (1994).

    Google Scholar 

  2. J. Priborsky, E. Mühlbachova J. Pharm. Pharmacol.42, 468 (1990).

    CAS  Google Scholar 

  3. P. J. Becci, M. J. Knickerbocker, E. L. Reagan, R. A. Parent, L. W. Burnette, Fundam. Appl. Toxicol.2, 273 (1982).

    Google Scholar 

  4. U. Hass, S. P. Lund, J. Elsner, Neurotoxicol. Teratol.16, 241 (1994).

    Article  CAS  Google Scholar 

  5. G. M. Solomon, E. P. Morse, M. J. Garbo, D. K. Milton, J. Occup. Environ Med.38, 705 (1996).

    Article  CAS  Google Scholar 

  6. H. Blome, M. Hennig, Staub-Reinhalt Luft44, 27 (1984).

    CAS  Google Scholar 

  7. B. Åkesson, K. Paulsson, Occup. Environ. Med.54, 236 (1997).

    Article  Google Scholar 

  8. C. Ursin, C. M. Hansen, J. W. Van Dyk, P. O. Jensen, I. J. Christensen, J Ebbehoej, Am. Ind. Hyg. Assoc. J.56, 651 (1995).

    CAS  Google Scholar 

  9. B. Åkesson, B. A. G. Jönsson, Drug Metab. Dispos.25, 267 (1997).

    Google Scholar 

  10. B. A. G. Jönsson, B. Åkesson, J. Chromatogr. submitted for publication.

  11. B. A. G. Jönsson, B. Åkesson, J. Chromatogr. in press.

  12. J. C. Miller, J. N. Miller, Statistics for Analytical Chemistry, Ellis Horwood, Chichester 1984.

    Google Scholar 

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Authors and Affiliations

  1. Department of Occupational and Environmental Medicine, Institute of Laboratory Medicine, University Hospital, 221 85, Lund, Sweden

    B. A. G. Jönsson & B. Åkesson

Authors
  1. B. A. G. Jönsson
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  2. B. Åkesson
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Jönsson, B.A.G., Åkesson, B. Analysis of 5-hydroxy-N-methyl-2-pyrrolidone and 2-hydroxy-N-methylsuccinimide in plasma. Chromatographia 46, 141–144 (1997). https://doi.org/10.1007/BF02495324

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  • DOI: https://doi.org/10.1007/BF02495324

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