Summary
The aim of this work was to compare the chemical composition of the underground parts (roots and rhizomes) ofGentiana cruciata L.,Gentiana pneumonanthe L., andGentiana asclepiadea L.— the three gentians native to Hungary—with that of the widely used stomachicGentiana lutea L., to determine which of the three Hungarian species could be used as a substitute forGentiana lutea in pharmaceutical preparations. The four gentians were compared by means of RPHPLC with diode-array detection (DAD) and electrospray ionization-mass spectrometric detection (ESI-MSD). The quantities of the lead compounds, the secoiridoid-glycosides, in 220 samples of the underground parts of gentians originating from several locations in Hungary, were determined by a more economical RPHPLC-DAD method. The occurrence of the characteristic compounds investigated—bitter principles and xanthones—in the underground parts of the speciesGentiana asclepiadea L. suggest it might be a potential replacement forGentiana lutea L. in pharmaceutical products.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Hegnauer, R., Ed.,Chemotaxonomie der Pflanzen, Birkhäuser, Basel,1989.
Hansel, R.; Rimpler, H.; Keller, K.; Schneider, G.,Hagers Handbuch, der Pharmazeutischen Praxis, Springer, Berlin,1993.
Kondo, Y.; Takano, F.; Hojo, H.Planta Med. 1994,60, 414–416.
Hostettmann, K.; Wagner, H.Phytochemistry 1977,16, 821–829.
Niessen, W.M.A.; van der Greef, J.Liquid Chromatography-Mass Spectrometry, Principles and Applications, Marcel Dekker, New York,1992.
Whitehouse, R.C.; Dreyer, R.N.; Yamashita, M.; Fenn, J.B.Anal. Chem. 1985,57, 675.
Wolfender, J.-L.; Rodriguez, S.; Hostettmann, K.Chromatographia 2001,54, 274–277.
Chevalley, I.; Marston, A.; Hostettmann K.J. Chromatogr. A 1998,794, 299–316.
Sticher, O.; Meier, B.Planta Med. 1980,40, 55–67.
Takino, Y.; Koshioka, M.; Kawaguchi, M.; Miyahara, T.; Tanizawa, H.; Ishii, Y.; Higashino, M.; Hayashi, T.Planta Med. 1980,38, 344–350.
Keller, F.J. Plant Phys. 1986,122, 473–476.
Schaufelberger, D.; Hostettmann, K.J. Chromatogr. 1985,346, 396–400.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Szucs, Z., Dános, B. & Nyiredy, S. Comparative analysis of the underground parts ofGentiana species by HPLC with diode-array and mass spectrometric detection. Chromatographia 56, S19–S23 (2002). https://doi.org/10.1007/BF02494108
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02494108