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Towards molecular-imprint based SPE of local anaesthetics

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Summary

The use of imprinted polymers for selective solid-phase extraction of local anaesthetics from human plasma was investigated. Methacrylic acid-ethylene glycol dimethacrylate based MIPs were developed by the alternative template approach using a structural analogue, pentycaine, as the template. The influence of the type and concentration of detergent in the buffer on selective and non-specific binding was investigated. Tween 20, Triton X-100 and Brij 35 were found to eliminate non-specific adsorption and leave selective imprint-analyte binding essentially unaffected. Efficient pre-concentration of ropivacaine and bupivacaine from plasma samples, both off-line prior to GC and in-line with LC-MS-MS, was demonstrated.

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Author notes

  1. L. Schweitz

    Present address: Product Analysis, AstraZeneca R&D Mölndal, 43183, Mölndal, Sweden

Authors and Affiliations

  1. DMPK & Bioanalytical Chemistry, AstraZeneca R&D Södertälie, 15185, Södertälje, Sweden

    L. I. Andersson, M. Abdel-Rehim & L. Nicklasson

  2. Technical Analytical Chemistry, University of Lund, 22100, Lund, Sweden

    L. Schweitz & S. Nilsson

Authors
  1. L. I. Andersson
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  2. M. Abdel-Rehim
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  3. L. Nicklasson
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  4. L. Schweitz
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  5. S. Nilsson
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Andersson, L.I., Abdel-Rehim, M., Nicklasson, L. et al. Towards molecular-imprint based SPE of local anaesthetics. Chromatographia 55 (Suppl 1), S65–S69 (2002). https://doi.org/10.1007/BF02493355

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  • DOI: https://doi.org/10.1007/BF02493355

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