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Adsorption kinetics of human serum albumin on various types of chromatographic support

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Summary

The adsorption of human, serum albumin (HSA) on various types of chromatographic support (C6 reversed-phase, anion-exchanger, immunoadsorbent) was studied by analysis of frontal breakthrough curves. These curves are first characterized by a sharp increase; a more diffuse profile is then observed at longer elution times. Data were analyzed using a bi-Langmuir kinetic model assuming irreversible adsorption on two types of binding site. Fitting the model to the experimental data enables determination of apparent adsorption rates and the saturation capacities of both types of binding site.

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Author notes

  1. A. Pantazaki

    Present address: Laboratory of Biochemistry, Aristotle University of Thessaloniki, 54006, Thessaloniki, Greece

Authors and Affiliations

  1. Laboratoire de Recherche sur les Polymères, CNRS, 2 rue Henry Dunant, 94320, Thiais, France

    A. Jaulmes & C. Vidal-Madjar

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  1. A. Jaulmes
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  2. C. Vidal-Madjar
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  3. A. Pantazaki
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Jaulmes, A., Vidal-Madjar, C. & Pantazaki, A. Adsorption kinetics of human serum albumin on various types of chromatographic support. Chromatographia 53 (Suppl 1), S417–S423 (2001). https://doi.org/10.1007/BF02490368

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  • DOI: https://doi.org/10.1007/BF02490368

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