Summary
A simple GC-MS method has been studied for the determination of aldoses and uronic acids in polysaccharide hydrolysates. Unlike other methods, the derivatisation procedure gives a single peak for each particular monosaccharide, including uronic acids. The method has been applied to plant gums used in decorative artefacts. A sample of gum, after acid hydrolysis, is first mercaptalated with a mixture of ethanethiol and trifluoroacetic acid (2∶1) and then derivatized in the same vessel. The obtained diethyldithioacetal trimethylsilylates or peracetates are separated by capillary column gas chromatography on SE-54 stationary phase. The chromatographic retention indices and El mass spectra of monosaccharide standards are reported together with composition data for six plant gums.
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Pitthard, V., Finch, P. GC-MS analysis of monosaccharide mixtures as their diethyldithioacetal derivatives: Application to plant gums used in art works. Chromatographia 53 (Suppl 1), S317–S321 (2001). https://doi.org/10.1007/BF02490349
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DOI: https://doi.org/10.1007/BF02490349