Abstract
A flavin-like green autofluorescent substance is noticed to occur in one of the flagella of flagellated cells in the Phaeophyceae, Chrysophyceae, Synurophyceae, Xanthophyceae and Prymnesiophyceae. In the phaeophycean swarmers the autofluorescence occurs in the posterior flagellum throughout its length. It is considered to be involved in the photoreception of phototaxis, since it almost always occurs in the swarmers which have a flagellar swelling and stigma and show phototaxis. In the phaeophycean swarmers, the stigma is shown to act as a concave reflector mirror focusing the reflection light onto the flagellar swelling. In the action spectrum studies, phaeophycean swarmers showed phototaxis between 370 and 520 nm, having two major peaks at 420 or 430 nm and 450 or 460 nm. Their responses were true phototactic and not photophobic. Rotation of the swarmer was shown to be essential in the photoreception ofEctocarpus gametes.
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References
Andersen, R.A. 1985. The flagellar apparatus of the golden algaSynura uvella: four absolute orientations. Protoplasma128: 94–108
— 1987. Synurophyceae classis nov., a new class of algae. Am. J. Bot.74: 337–353.
Belcher, J.H. andE.M. Swale. 1967.Chromulina placentula sp. nov. (Chrysophyceae), a freshwater nannoplankton flagellate. Br. phycol. Bull.3: 257–267.
Benedetti, P.A. andA. Checucci. 1975. Paraflagellar body (PFB) pigments studied by fluorescence microscopy inEuglena gracilis. Plant Sci. Lett.4: 47–51.
— andF. Lenci. 1977. In vivo microspectrofluorometry of photoreceptor pigments inEuglena gracilis. Photochem. Photobiol.26: 315–318.
Bold, H.C. andM.J. Wynne. 1985. Introduction to the Algae. 2nd. ed. Prentice-Hall, Englewood Cliffs, New Jersey.
Cavalier-Smith, T. 1986. The kingdom Chromista: Origin and systematics.In F.E. Round and D.J. Chapman, ed., Progress in Phycological Research, vol. 4, pp. 309–347. BioPress, Bristol.
— 1989. The kingdom Chromista.In J.C. Green, B.S.C. Leadbeater, W.I. Diver, ed., The Chromophyte Algae: Problems and Perspectives, pp. 381–407. Clarendon Press, Oxford.
Clayton, M.N. 1989. Brown algae and chromophyte phylogeny.In J.C. Greenet al., ed., The Chromophyte Algae: Problems and Perspectives. pp. 229–253. Clarendon Press, Oxford.
Coleman, A.W. 1988. The autofluorescent flagellum: a new phylogenetic enigma. J. Phycol.24: 118–120.
Diehn, B. 1969. Action spectra of the phototactic responses inEuglena. Biochim. Biophys. Acta177: 136–143.
Dodge, J.D. 1973. The Fine Structure of Algal cells. Academic Press, London, 261 pp.
Forward, R.B. 1973. Phototaxis in a dinoflagellate: action spectra as evidence for a two-pigment system. Planta (Berl.)111: 167–178.
— 1974. Phototaxis by the dinoflagellateGymnodinium splendens Lebour. J. Protozool.21: 312–315.
Foster, K.W. andR.D. Smyth. 1980. Light antennas in phototactic algae. Microbiol. Rev.44: 572–630.
Green, J.C. 1980. The fine structure ofPavlova pinguis Green and a preliminary survey of the order Pavlovales (Prymnesiophyceae). Br. Phycol. J.15: 151–191.
— andD.J. Green. 1977. The ultrastructure and taxonomy ofDiacronema vlkianum (Prymnesiophyceae) with special reference to the haptonema and flagellar apparatus. J. mar. biol. Ass. U.K.,57: 1125–1136.
Häder, D-P., M. Lebert andM.R. Di Lena. 1986. New evidence for the mechanism of phototactic orientation ofEuglena gracilis. Current Microbiology.14: 157–163.
Halldal, P. 1958. Action spectra of phototaxis and related problems in Volvocales,Ulva-gametes and Dinophyceae. Physiol. Plant.11: 118–153.
— 1961. Ultraviolet action spectra of positive and negative phototaxis inPlatymonas subcordiformis. Physiol. Plant.14: 133–139.
Henry, E.C. andK.M. Cole. 1982. Ultrastructure of swarmers in the Laminariales (Phaeophyceae) I. zoospores. J. Phycol.8: 550–569.
Hibberd, D.J. 1972. Observations on the cytology and ultrastructure of the new class Eustigmatophyceae. Ann. Bot.36: 49–71.
Inouye, I., Z. Zhang, M. Enomoto andM. Chihara. 1990. Cell structure of an unusual chrysophytePhaeaster pascheri with particular emphasis on the flagellar apparatus architecture. Jpn. J. Phycol.38: 11–24.
Kawai, H. 1988. A flavin-like autofluorescent substance in the posterior flagellum of golden and brown algae. J. Phycol.24: 114–117.
— andI. Inouye. 1989. Flagellar autofluorescence in forty-four chlorophyllc-containing algae. Phycologia28: 222–227.
—,M. Kubota, T. Kondo, M. Watanabe. 1991. Action spectra for phototaxis in zoospores of the brown algaPseudochorda gracilis. Protoplasma161: 17–22.
—,D.G. Müller, E. Fölster andD.-P. Häder. 1990. Phototactic responses in the gametes of the brown alga,Ectocarpus siliculosus. Planta182: 292–297.
Kreimer, G., H. Kawai, D.G. Müller andM. Melkonian. 1991. Reflective properties of the stigma in male gametes ofEctocarpus siliculosus (Phaeophyceae) studied by confocal laser scanning microscopy. J. Phycol.27: 268–276.
— andM. Melkonian. 1990. Reflection confocal laser scanning microscopy of eyespots in flagellated green algae. Eur. J. Cell Biol.53: 101–111.
Kristiansen, J. 1990. Phylum Chrysophyta.In L. Marguliset al., eds., Handbook of Protoctista. pp. 438–453. Jones and Bartlett Publishers, Boston.
— andP.L. Walne. 1976. Structural connections between flagellar base and stigma inDinobryon. Protoplasma89: 371–374.
——. 1977. Fine structure of photo-kinetic systems inDinobryon cylindricum var.alpinum (Chrysophyceae). Br. Phycol. J.12: 329–341.
Manton, I. andH.A. von Stosch. 1966. Observations on the fine structure of the male gamete of the marine centric diatomLithodesmium undulatum. J.R. Micros. Soc.85: 119–134.
Mast, S.O. 1911. Light and Behavior of Organisms. John Willey & Sons, New York.
— 1927. The structure and function of the eyespot in unicellular and colonial organisms. Arch. Protistenkd.60: 197–220.
Moestrup, Ø. 1982. Flagellar structure in algae: a review, with new observations particularly on the Chrysophyceae, Phaeophyceae (Fucophyceae), Euglenophyceae, andReckertia. Phycologia21: 427–528.
Müller, D.G., I. Maier andH. Müller. 1987. Flagellum autofluorescence and photoaccumulation in heterokont algae. Photochem. Photobiol.46: 1003–1008.
Nultsch, W., G. Throm andI. von Rimscha. 1971. Phototaktische Untersuchungen anClamydomonas reinhardii Dangeard in homokontinuierlicher Kultur. Arch. Mikrobiol.80: 351–369.
Rouiller, C. andE. Faure-Fremiet. 1958. Structure fine d'un flagelle chrysomonadien:Chromulina psammobia. Exp. Cell. Res.14: 47–67.
Schmidt, W., P. Galland, H. Senger andM. Furuya. 1990. Microspectrophotometory ofEuglena gracilis. Pterin-and flavin-like fluorescece in the paraflagellar body. Planta182: 375–381.
South, G.R. andA. Whittick. 1987. Introduction to Phycology. Blackwell Scientific Publications, Oxford.
Watanabe, M. andM. Furuya. 1974. Action spectrum of phototaxis in a cryptomonad alga,Cryptomonas sp. Plant Cell Physiol.15: 413–420.
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Recipient of the Botanical Society Award for Young Scientists, 1991.
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Kawai, H. Green flagellar autofluorescence in brown algal swarmers and their phototactic responses. Bot Mag Tokyo 105, 171–184 (1992). https://doi.org/10.1007/BF02489413
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DOI: https://doi.org/10.1007/BF02489413