Phototactic responses in the gametes of the brown alga, Ectocarpus siliculosus

Abstract

The action spectrum of phototaxis was determined and the photoreceptive mechanism was studied in Ectocarpus gametes (Ectocarpales, Phaeophyceae) using a computerized cell-tracking system. The fine structures of the stigma and the flagellar swelling were analyzed, and the reflective function of the stigma was demonstrated for the first time. Under monochromatic light stimulation, Ectocarpus gametes show mainly positive phototaxis between 370 nm and 520 nm. The action spectrum has a minor peak near 380 nm, and two major peaks at 430 nm and 450 nm or 460 nm and a shoulder at 470 nm adjoining a remarkable depression near 440 nm. Under unilateral stroboscopic illumination with more than four pulses per second, the gametes show clear phototaxis. However, the response is disturbed at lower frequencies. Addition of methyl cellulose, which increases the viscosity of the medium and slows down gamete rotation, decreases the threshold frequency. These results indicate that rotation of the gamete plays an essential role in the photoreceptive mechanism. Under equal intensities of bilateral illumination at an angle of 90°, most of the gametes swim on the resultant between the two light beams. This response is disturbed when the angle of the two light beams is as large as 120°. Observations by transmission electron microscopy show that the flagellar swelling fits precisely into a concave depression of the chloroplast at the central region of the stigma. Electron-dense material is present in that sector of the flagellar swelling which faces away from the stigma. Epifluorescence microscopy without a barrier filter and epipolarization microscopy reveal that stigmata reflect blue light. A hypothesis is formulated which discusses the possibility that the reflected light is focused onto the flagellar swelling.