Abstract
The experiments to assess the role of iron in morphogenesis of microspore-derived plantlets in anther cultures ofDatura metel revealed that even on iron-deficient medium androgenesis is initiated but the induced units fail to grow beyond multicellular stage, if anthers are not transferred to iron-incorporated medium. Another important conclusion drawn from an experiment involving weekly transfer of anthers from nutrient medium lacking both iron source (ferrous sulfate, FeSO4·7H2O, 10−4 M) and chelate (disodium salt of ethylene diamine tetraacetic acid, C10H14N2Na2O8·2H2O, 10−4 M) to that containing both of these or vice-versa, is that iron if present during the initial culture period, in fact affects the plantlet yield adversely. Thus, anthers cultured on iron-deficient medium, for initial one or two weeks and then transferred to iron-containing medium, yielded higher number of plantlets than those developed from anthers cultured continuously on complete basal medium having both iron and chelate.
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Babbar, S.B., Gupta, S.C. Obligatory and period specific requirement of iron for microspore embryogenesis inDatura metel anther cultures. Bot Mag Tokyo 99, 225–232 (1986). https://doi.org/10.1007/BF02488823
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DOI: https://doi.org/10.1007/BF02488823