Abstract
The inhibitory action of prostaglandin D2 (PGD2) and its effect on the cell cycle were examined in cell lines SW480 and LS174T of human colon cancer. The growth of the cell lines were assessed 24h and 48h after the addition of 1.0μg/ml and 10.0μg/ml PGD2. The growth of SW480 cells and 24h and 48h after the addition of 10.0μ/ml, while that of LS174T was inhibited by both doses after 24h and 48h. S-Phase DNA synthesis in the SW480 cells was significantly blocked 24h after the addition of 10.0μg/ml PGD2. The cell cycle of LS174T cells was arrested at the G0+G1 phase 24h after the addition of 1.0μg/ml and 10.0μg/ml PGD2. The correlation between hepatic metastasis and PGD2 concentration in human cancer tissue was examined. The mean value of PGD2 concentrations in the primary cancer tissue was significantly lower in the hepatic metastasis group than that in the group without hepatic metastasis. These findings suggest that measuring the PGD2 in cancer tissue may be useful for detecting and predicting the hepatic metastasis from human colorectal cancer.
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References
Smith JB, Silver MJ, Ingerman CM, Kocsis JJ (1974) Prostaglandin D2 inhibits the aggregation of human platelets. Thromb Res 5:291–299
Whittle BJR, Moncada S, Vane JR (1978) Comparison of the effects of prostacyclin (PGI2), prostaglandin E1 and D2 on platelet aggregation in different species. Prostaglandins 16:373–388
Shimizu T, Mizuno N, Amano T, Hayashi O (1979) Prostaglandin D2, a neuromodulator. Proc Natl Acad Sci USA 76:6231–6234
Fukushima M, Kato T, Ueda R, Ota K, Narumiya S, Hayashi O (1982) Prostaglandin D2, a potential antineoplastic agent. Biochem Biophys Res Commun 105:956–964
Simmet T, Jaffe BM (1983) Inhibition of B-16 melanoma growth in vitro by prostaglandin D2. Prostaglandins 25:47–54
Sakai T, Yamaguchi N, Morimasa Y, Sekiguchi S, Fujii G, Nishino H (1984) Prostaglandin D2 inhibits the proliferation of human malignant tumor cells. Prostaglandins 27:17–26
Fitzpatrick FA, Stringfellow DA (1979) Prostaglandin D2 formation by malignant melanoma cells correlates inversely with cellular metastatic potential. Proc Natl Acad Sci USA 76:1765–1769
Stringfellow DA, Fitzpatrick FA (1979) Prostaglandin D2 controls pulmonary metastasis of malignant melanoma cells. Nature 282:76–78
Nakahata N, Ono T, Nakanishi H (1987) Contribution of prostaglandin E2 to bradykinin-induced contraction in rabbit urinary detrusor. Jpn J Pharmacol 43:351–359
Santoro MG, Jaffe BM (1979) Inhibition of friend erythroleukemia-cell tumors in vivo by a synthetic analogue of prostaglandin E2. Br J Cancer 39:408–413
Santoro MG, Philpott GW, Jaffe BM (1976) Inhibition of tumor growth in vivo and in vitro by prostaglandin E. Nature 263:777–779
Santoro MG, Philpott GW, Jaffe BM (1977) Inhibition of B-16 melanoma growth in vivo by a synthetic analogue of PGE2. Cancer Res 37:3774–3779
Honda C, Tabuchi K (1986) Effects of prostaglandin D2 on cultured glioma cells (in Japanese with English abstract) Gan to Kagakuryouhou (Jpn J Cancer Chemother) 13:2758–2765
Bhuyan BK, Adams EG, Badiner GJ, Li LH, Barden K (1986) Cell cycle effects of prostaglandin A1, A2, and D2 in human and murine melanoma cells in culture. Int J Cancer 46:1689–1693
Ehara H (1989) An experimental study on antitumor effect of prostaglandin D2 (in Japanese with English abstract). Nippon Geka Gakkai Zasshi (J Jpn Surg Soc) 90:980–987
Oda N, Sarashina H, Saitoh N, Nunomura M, Yokoyama M, Ihara M, Nakayama H, Shirai Y, Ohmori T, Takiguchi N, Miyazaki M, Okui K (1990) A clinicopathological feature of liver metastasis in colorectal cancer (in Japanese with English abstract). Nippon Syokaki Geka Gakkai Zassi (Jpn J Gastroenterol Surg) 23:2251–2255
Shirai S (1991) Clinicopathologic study on liver metastasis of colorectal cancer with special reference to subserosal venous invasion of primary lesion and liver metastasis (in Japanese with English abstract). Nippon Daicho Koumonbyou Gakkai Zassi (J Jpn Soc Coloproctol) 44:1005–1018
Yoshida T, Kanazawa M, Mizunuma H, Watanabe H, Kikuchi Y, Satoh H, Andoh Y, Takeuchi S, Tsuchiya A, Abe R (1995) Malignant potential and correlation between prostaglandins, c-erbB-2 oncoprotein expression and ploidy pattern in colon cancer (in Japanese with English abstract). Nippon Syokaki Geka Gakkai Zassi (Jpn J Gastroenterol Surg) 28:673–678
Gasic GJ, Gasic TB, Galanti N, Johnson T, Murphy S (1973) Platelet-tumor-cell interactions in mice. The role of platelets in the spread of malignant disease. Int J Cancer Res 11:704–718
Narumiya S, Toda N (1985) Different responsiveness of prostaglandin D2-sensitive systems to prostaglandin D2 and its analogues. Br J Pharmacol 85:367–375
Fitzpatrick FA, Wynalda MA (1983) Albumin-catalyzed metabolism of prostaglandin D2. J Biol Chem 258:11713–11718
Narumiya S, Fukushima M (1985) Δ12-Prostaglandin J2, an ultimate metabolite of prostaglandin D2 exerting cell growth inhibition. Biochem Biophys Res Commun 127:739–745
Hirata Y, Hayashi H, Ito S, Kikawa Y, Ishibashi M, Sudo M, Miyazaki H, Fukushima M, Narumiya S, Hayaishi O (1988) Occurrence of 9-deoxy-Δ9, Δ12-13,14-dihydroprostaglandin D2 in human urine. J Biol Chem 263:16619–16625
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Yoshida, T., Ohki, S., Kanazawa, M. et al. Inhibitory effects of prostaglandin D2 against the proliferation of human colon cancer cell lines and hepatic metastasis from colorectal cancer. Surg Today 28, 740–745 (1998). https://doi.org/10.1007/BF02484622
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DOI: https://doi.org/10.1007/BF02484622