Abstract
We studied the transfer factor (TF) with regard toin vivo andin vitro restoration of cell mediated immunity (CMI) in lung cancer patients. Twenty-eight lung cancer patients who had undergone resection were the recipients and 30 household contact family members with a positive reactivity to lung cancer extract were the donors of TF. Immunologic status was evaluated by delayed type cutaneous hypersensitivity (DTH), peripheral T lymphocyte number, PHA lymphocyte blastogenesis, serum blocking activity (SBA) and leucocyte adherence inhibition (LAI) test. When TF was administered twice subcutaneously to the patients, there was a statistically significant restoration or augmentation of DTH, PHA lymphocyte blastogenesis and abrogation of SBA, particularly in patients with suppressed CMI. These results suggest that it was the TF obtained from relatives of lung cancer patients with positive reactivity to tumor associated antigens restored or augmented tumor specific and nonspecific CMI in these lung cancer patients.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lawrence, H. S.: The transfer in humans of delayed skin sensitivity to streptococcal M substance and to tuberculin with disrupted leucocytes. J. Clin. Invest. 34: 219–230, 1955.
Levin, A. S., Spitler, L. E., Stites, D. P. and Fudenberg, H. H.: Wiskott-Aldrich syndrome, a genetically determined cellular immunologic deflciency: Clinical and laboratory responses to therapy with Transfer Factor. Proc. Nat. Acad. Sci. 67: 821–828, 1970.
Lawrence, H. S. and Zweiman, B.: Transfer Factor deficiency response-A mechanism of energy in Boeck's sarcoid. Trans. Assoc. Amer. Phys. 81: 240–248, 1968.
Schulkind, M. L., Adler, W. H., Altemeier, W. A. and Ayoub, E. M.: Transfer Factor in the treatment of a case of chronic mucocutaneous candidiasis. Cell. Immunol. 3: 606–615, 1972.
Brandes, L. J., Galton, D. A. G. and Wiltshow, E.: New approach to immunotherapy of melanoma. Lancet 2: 293–295, 1971.
Lo Buglio, A. F., Neidhart, J. A., Hilberg, R. W., Metz, E. N. and Balcerzak, S. P.: The effect of Transfer Factor therapy on tumor immunity in alveolar soft part sarcoma. Cell. Immunol. 7: 159–165, 1973.
Levin, A. S., Byers, V. S., Fudenberg, H. H., Wybran, J., Hackett, A. J., Johnston, J. O. and Spitler, L. E.: Osteogenic sarcoma, Immunologic parameters before and during immunotherapy with tumor-specific Transfer Factor. J. Clin. Invest. 55: 487–499, 1975.
Fujisawa, T. and Yonemoto, R. H.: Transfer Factor immunotherapy in patients with solid tumors. Gan-no Rinsho. (Jpn. J. Cancer Clin.) 24: 445–452, 1978. (English Abst.)
Yonemoto, R. H., Fujisawa, T. and Waldman, S. R.: Selection of donors for Transfer Factor. Proc. Amer. Assoc. Cancer Res. 17: 150, (abst. 600), 1976.
Fujisawa, T., Yamaguchi, Y. and Yonemoto, R. H.: Leukocyte adherence inhibition directed to soluble tumor associated antigens in patients with malignant tumors and their household contacts. Rinsho Men-eki. (Clin. Immunol.) 10: 1181–1186, 1978. (English Abst.)
Fujisawa, T., Waldman, S. R. and Yonemoto, R. H.: Transfer Factor in vitro abrogation of blocking factors and amplification of immunoreactivity directed to soluble tumor associated antigens in the Leucocyte Adherence Inhibition assay. Cancer Immunol. Immunother. 4: 77–86, 1978.
Katsuki, H., Shimada, K., Koyoma, A., Okita, M. Yamaguchi Y. and Okamoto, T.: Long-term intermittent adjuvant chemotherapy for primary, resected lung cancer. J. Thorac. Cardiovasc. Surg. 70: 590–605, 1975.
Fujisawa, T., Yamaguchi, Y., Arita, M., Satoh, N., Shiba, M., Matsumoto, K. and Murota, Y.: Cell mediated immunity of the patients with primary resected lung cancer treated with long term intermittent adjuvant chemotherapy (LT-IC). Gan to Kagakuryoho. (Cancer and Chemotherapy) 7: 1068–1075, 1980. (English Abst.)
Meltzer, M. S., Leonard, E. J., Rapp, H. J. and Boros, T.: Tumor-specific antigen solubilized by hypertonic potassium chloride. J. Natl. Cancer Inst. 47: 703–709, 1971.
Fujisawa, T., Waldman, S. R. and Yonemoto, R. H.: Leucocyte Adherence Inhibition by soluble tumor antigens in breast cancer patients. Cancer 39: 506–513, 1977.
Eilber, F. R., Nizze, J. A. and Morton, D. L.: Sequential evaluation of general immune competence in cancer patients: Correlation with clinical course. Cancer 35: 660–665, 1975.
Potvin, C., Tarpley, J. L. and Chretien, P.: Thymus derived lymphocytes in patients with solid malignancies. Clin. Immunol. Immunopath. 3: 476–481, 1975.
Thurman, G. B., Strong, D. M., Ahmed, A., Green, S. S., Sell, D. W., Hartzman, R. J. and Bach, F. H.: Human-mixed lymphocyte cultures, evaluation of a microculture technique utilizing the multiple automated sample harvester (MA-SH). Clin. Exp. Immuno. 15: 289–302, 1973.
Fujisawa, T., Yonemoto, R. H. and Waldman, S. R.: Comparison of serum blocking factors on leucocyte adherence inhibition and PHA lymphocyte blastogenesis in breast cancer patients. Jpn. J. Surg. 11: 406–412, 1981.
Vetto, R. M., Burger, D. R., Vandenbark, A. A. and Nolt, J.: Influence of serum blocking factors on cancer patients undergoing immunotherapy. Amer. J. Surg. 130: 237–243, 1975.
Halliday, W. J. and Miller, S.: Leucocyte adherence inhibition: A simple test for cell- mediated tumor immunity and serum blocking factors. Int. J. Cancer 9: 477–483, 1977.
Brandes, L. J. and Goldenberg, G. L.: In vitro transfer of cellular immunity against nasopharyngeal carcinoma using transfer factor from donors with Epstein-Barr virus antibody activity. Cancer Res. 34: 3095–3101, 1974.
Saito, K., Tamaki, N. and Gottlieb, A. A.: Inhibition of DNA synthesis in lymphocytes by dialyzable components of human leucocyte extract. Cell Immunol. 31: 311–320, 1977.
Burger, D. R., Vandenbark, A. A., Dunnick, W., Kraybill, W., Davis, G. D. and Vetto, M.: Human transfer factor: Structural properties suggested by HRP chromatography and enzymatic sensitivities. J. Immunol. 122: 1091–1098, 1979.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fujisawa, T., Yamaguchi, Y. & Kimura, H. Transfer factor in restoration of cell mediated immunity in lung cancer patients. The Japanese Journal of Surgery 13, 304–311 (1983). https://doi.org/10.1007/BF02469511
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02469511