Skip to main content
SpringerLink
Log in

Comparison of serum blocking factors on leucocyte adherence inhibition and PHA lymphocyte blastogenesis in breast cancer patients

  • Original Articles
  • Published:
The Japanese journal of surgery Aims and scope Submit manuscript

Abstract

Specific and nonspecific serum blocking factors (SBF) in sera from 48 breast cancer patients were examined to evaluate the correlation of SBF to the tumor burden. Specific SBF in leucocyte adherence inhibition (LAI) assay was demonstrated in 15 of 21 patients with a recurrence, in five of 11 with local or regional involvement and in 3 of 16 with no evidence of the disease (NED). The difference between patients with recurrence and with NED was significant (P<0.005). SBF in LAI and lymphocyte blasto-genesis correlated in 29 of 48 (60%) patients (both positive 9 of 48, both negative 20 of 48). However, SBF in LAI was demonstrated in 14 of 48 (29%) patients whose sera showed no SBF in lymphocyte blastogenesis. These results indicate that SBF in LAI and lymphocyte blastogenesis are either induced or derived from breast cancer tissue. SBF in LAI correlates closely with the tumor burden, more so than do SBF in lymphocyte blastogenesis. The blocking assays have a potential use for prognostication and a guide for clinical therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hellström, I. and Hellström, K.E.: Studies on cellular immunity and its serum-mediated inhibition in Moloney-virus-induced mouse sarcomas. Int. J. Cancer 4: 587–600, 1969.

    PubMed  Google Scholar 

  2. Hellström, I., Sjogren, H.O., Warner, G. and Hellström, K.E.: Blocking of cell-mediated tumor immunity by sera from patients with growing neoplasma. Int. J. Cancer 7: 226–237, 1971.

    PubMed  Google Scholar 

  3. Heppner, G.H., Stolbach, L., Byrne, M., Cummings, F.J., McDonough, E. and Calabresi, P.: Cell-mediated and serum blocking reactivity to tumor antigens in patients with malignant melanoma. Int. J. Cancer 11: 245–260, 1973.

    PubMed  CAS  Google Scholar 

  4. Halliday, W.J.: Blocking effect of serum from tumor bearing animals on macrophage migration inhibition with tumor antigens. J. Immunol. 106: 855–857, 1971.

    PubMed  CAS  Google Scholar 

  5. Maluish, A. and Halliday, W.J.: Cell-mediated immunity and specific serum factors in human cancer: The Leucocyte Adherence Inhibition Test. J. Natl. Cancer Inst. 52: 1415–1420, 1974.

    PubMed  CAS  Google Scholar 

  6. Tamerius, J., Nepom, J., Hellström, I. and Hellström, K.E.: Tumor associated blocking factors: Isolation from sera of tumor bearing mice. J. Immunol. 116: 724–730, 1976.

    PubMed  CAS  Google Scholar 

  7. Bansal, S. and Sjogren, H.O.: Correlation between changes in anti-tumor immune parameters and tumor growthin vivo in rats. Fed. Proc. 32: 165–172, 1973.

    PubMed  CAS  Google Scholar 

  8. Catalona, W.J., Sample, W.F. and Chretien, P.B.: Lymphocyte reactivity in cancer patients: Correlation with tumor histology and clinical stage. Cancer 31: 65–71, 1973.

    Article  PubMed  CAS  Google Scholar 

  9. Cooperband, S.R., Bondevik, H., Schmid, K. and Mannick, J.A.: Transformation of human lymphocytes: Inhibition by homologous alpha globulins. Science 159: 1243–1244, 1968.

    PubMed  CAS  Google Scholar 

  10. Nimberg, R.B., Glasgow, A.H., Menzoian, J.O., Constantian, M.B., Cooperband, S.R., Mannick, J.A. and Schmid, K.: Isolation of an immunosuppressive peptide fraction from the serum of cancer patients. Cancer Res. 35: 1489–1494, 1975.

    PubMed  CAS  Google Scholar 

  11. Meltzer, M.S., Leonard, E.J., Rapp, H.J. and Borsos, T.: Tumor specific antigen solubilized by hypertonic potassium chloride. J. Natl. Cancer Inst. 47: 703–709, 1971.

    PubMed  CAS  Google Scholar 

  12. Fujisawa, T., Waldman, S.R. and Yonemoto, R.H.: Leucocyte adherence inhibition by soluble tumor antigens in breast cancer patients, Cancer 39: 134–141, 1977.

    Article  Google Scholar 

  13. Yonemoto, R.H., Fujisawa T. and Waldman, S.R.: Effect of serum blocking factors on leucocyte adherence inhibition in breast cancer patients. Specificity and correlation with tumor burden. Cancer 41: 1289–1297, 1978.

    Article  PubMed  CAS  Google Scholar 

  14. Thurman, G.B., Strong, D.M., Ahmed, A., Green, S.S., Sell, K.W., Hartzman, R.J. and Bach, F.H.: Human mixed lymphocyte cultures, evaluation of a microculture technique utilizing the multiple automated sample harvester (MASH). Clin. Exp. Immunol. 15: 289–302, 1973.

    PubMed  CAS  Google Scholar 

  15. Boyum, A: Separation of leucocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21 Suppl. 97, 1968.

  16. Vetto, R.M., Burger, D.R., Vandenbark, A.A. and Nolte, J.: Influence of serum blocking factors on cancer patients undergoing immunotherapy. Amer. J. Surg. 130: 237–243, 1975.

    Article  PubMed  CAS  Google Scholar 

  17. Humphrey, G.B., Lankford, J., Oleinick, S. and Nitschke, R.: Leukemic serum inhibition of phytohemagglutinin induced transformation. J. Immunol. 113: 63–69, 1974.

    PubMed  CAS  Google Scholar 

  18. Fujisawa, T., Waldman, S.R. and Yonemoto, R.H.: Effect of serum factors and cell washings on leucocyte adherence inhibition in breast cancer patients. Gann 71: 165–172, 1980.

    PubMed  CAS  Google Scholar 

  19. Tanaka, F., Yonemoto, R.H., Waldman, S.R.: Blocking factors in sera of breast cancer patients. Cancer 43: 838–847, 1979.

    Article  PubMed  CAS  Google Scholar 

  20. Grosser, N. and Thomson, D.M.P.: Tube leucocyte (monocyte) adherence inhibition assay for the detection of anti-tumor immunity III. “Blockade” of monocyte reactivity by excess free antigen and immune complexes in advanced cancer patients. Int. J. Cancer 18: 58–66, 1976.

    PubMed  CAS  Google Scholar 

  21. Mantovani, A. and Spreafico, F.: On the nature of blocking factors and their lymphoid target cells in an allogeneic tumor system. Europ. J. Cancer 11: 451–455, 1975.

    CAS  Google Scholar 

Download references

Author information

Author notes

  1. Takehiko Fujisawa

    Present address: Department of Surgery, Institute of Pulmonary Cancer Research, Chiba University School of Medicine, 1-8-1, Inohana, 280, Chiba, Japan

Authors and Affiliations

  1. Department of General and Oncologic Surgery, City of Hope National Medical Center, 91010, California, Duarte, USA

    Takehiko Fujisawa, Robert H. Yonemoto & Stephen R. Waldman

Authors
  1. Takehiko Fujisawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert H. Yonemoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stephen R. Waldman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujisawa, T., Yonemoto, R.H. & Waldman, S.R. Comparison of serum blocking factors on leucocyte adherence inhibition and PHA lymphocyte blastogenesis in breast cancer patients. The Japanese Journal of Surgery 11, 406–412 (1981). https://doi.org/10.1007/BF02469023

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02469023

Key Words

Search

Navigation