Abstract
A small percentage of the Western population carries antibodies in the peripheral blood, which are able to kill human tumors such as neuroblastoma or melanoma. Several observations indicate that these antibodies, preferentially of IgM isotype, belong to the class of naturally occurring antibodies. Here, we describe two screening methods for the detection and quantification of such antibodies in human blood samples: a cellular ELISA technique and a flow cytometric assay, based on intercalation of fluorescent propidium iodide into the DNA of dying or dead cells.
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References
Schwartz-Albiez R (2012) Naturally occurring antibodies directed against carbohydrate antigens. In: Lutz HU (ed) Naturally occurring antibodies (Nabs). Springer, New York, NY. Adv Exp Med Biol 750:27–43. doi:10.1007/978-1-4614-3461-0_3
Devarapu SK, Mamidi S, Plöger F, Dill O, Blixt O, Kirschfink M et al (2016) Cytotoxic activity against human neuroblastoma and melanoma cells mediated by IgM antibodies derived from peripheral blood of healthy donors. Int J Cancer 138(12):2963–2973. doi:10.1002/ijc 30025.
Ollert MW, David K, Schmitt C, Hauenschild A, Bredehorst R, Erttmann R et al (1996) Normal human serum contains a natural antibody cytotoxic for human neuroblastoma cells. Proc Natl Acad Sci U S A 93:4498–4503
Vollmers HP, Brändlein S (2005) The “early birds”: natural IgM antibodies and immune surveillance. Histol Histopathol 20:927–937
Bologna L, Gotti E, Mangani M, Rambaldi A, Intermesoli T, Introna M et al (2011) Mechanism of action of type II, glycoenineered, anti-CD20 monoclonal antibody GA101 in B-chronic lymphocytic leukemia whole blood assays in comparison with Rituximab and Alemtuzumab. J Immunol 186:3762–3769
Acknowledgment
This work has been supported by a grant of the BMBF (German Ministry of Education and Research), grant number 0315340.
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Schwartz-Albiez, R., Dill, O. (2017). Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma. In: Kaveri, S., Bayry, J. (eds) Natural Antibodies. Methods in Molecular Biology, vol 1643. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7180-0_8
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DOI: https://doi.org/10.1007/978-1-4939-7180-0_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7180-0
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