Summary
Capillary electrophoresis (CE) has proved to be a fast and convenient method for the determination of the dissociation constants of non-UV absorbing solutes in the acidic pK A range (2.0–2.5).
The electroosmotic flow was reversed by washing the capillary with 0.2% polybren aqueous solution. A series of background electrolytes was prepared with phenylphosphonic acid (pK A=1.29) and β-alanine (pK A=3.55) with the same ionic strength and a high buffer capacity in order to improve the repeatability (0.1–0.2 %) of the electrophoretic mobility and to determine the values of pK A accurately.
This procedure was applied to the determination of the dissociation constants of several alkyl-alkylphosphonic acids whose pK A values have not yet been published in the literature. In this work, their dissociation constants have been found to vary between 1.91 and 2.34 for alkyl-methylphosphonic acids and between 2.10 and 2.38 for alkyl-ethylphosphonic acids.
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Mercier, JP., Morin, P., Dreux, M. et al. Determination of weak (2.0–2.5) dissociation constants of non-UV absorbing solutes by capillary electrophoresis. Chromatographia 48, 529–534 (1998). https://doi.org/10.1007/BF02466645
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DOI: https://doi.org/10.1007/BF02466645