Summary
An isogenic pair ofEscherichia coli strains, one carrying anrnc + and the other anrnc − allele (a mutation which reduces the level of ribonuclease III), was compared. Thernc − strain fails to grow at very elevated temperatures (forE. coli) while thernc + strain does grow exponentially.
Assaying the residual RNase III, like activity in extracts of thernc − strain at different pHs and at different temperatures suggested that this residual RNase III like activity is not due to RNase III. This raised the possibility that thernc − strain is devoid of any RNase III activity in the cell. Comparing the decay of newly synthesized RNA and functional decay of β-galactosidase mRNA in such strains revealed that in both strains these parameters proceed in similar rates, which suggests that RNase III is not involved in the metabolism of mRNA. During carbon starvation preexisting total RNA, as well as 23S and 16S rRNA, decay faster in thernc − strain, thus eliminating the possibility that RNase III is the endoribonuclease which initiates the decay of rRNA during starvation (Kaplan and Apirion, 1975a).
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References
Altman, S.: Biosynthesis of transfer RNA inEscherichia coli. Cell4, 21–29 (1975)
Altman, S., Robertson, H.D.: RNA precursor molecules and ribonucleases inE. coli. Molec. Cell. Biochem.1, 83–93 (1973)
Apirion, D.: Altered ribosomes in a suppressor strain ofEscherichia coli. J. molec. Biol.16, 285–301 (1966)
Apirion, D.: Degradation of RNA inEscherichia coli. A hypothesis. Molec. gen. Genet.122, 313–322 (1973)
Apirion, D., Watson, N.: Analysis of anEscherichia coli strain carrying physiologically compensating mutations one of which causes an altered ribonuclease III. Molec. gen. Genet132, 89–104 (1974)
Apirion, D., Watson, N.: Unaltered stability of newly synthesized RNA in strains ofEscherichia coli missing a ribonuclease specific for doublestranded RNA. Molec. gen. Genet.136, 317–326 (1975a)
Apirion, D., Watson, N.: Mapping and some characterization of a mutation inEscherichia coli that reduces the level of RNase III; a ribonuclease specific for double stranded RNA. J. Bact.124, 317–324 (1975b)
Crouch, R.J.: Ribonuclease III does not degrade deoxyribonucleic acid-ribonucleic acid hybrids. J. biol. Chem.249, 1314–1316 (1974)
Gegenheimer, P., and Apirion, D.:Escherichia coli ribosomal RNAs are not cut from an intact precursor molecule. J. biol. Chem.250, 2407–2409 (1975)
Kaplan, R., Apirion, D.: The involvement of ribonuclease I, ribonuclease II, and polynucleotide phosporylase in the degradation of stable ribonucleic acid during carbon starvation inEscherichia coli. J. biol. Chem.249, 149–151 (1974)
Kaplan, R., Apirion, D.: The fate of ribosomes inEscherichia coli cells starved for a carbon source. J. biol. Chem.250, 1854–1863 (1975)
Kaplan, R., Apirion, D.: Decay of ribosomal ribonucleic acid inEscherichia coli cells starved for various nutrients. J. biol. Chem.250, 3174–3178 (1975)
Kindler, P., Keil, T.U., Hofschneider, P.H.: Isolation and characterization of a ribonuclease III deficient mutant ofEscherichia coli. Molec. gen. Genet.126, 53–69 (1973)
Kinscherf, T.G., Apirion, D.: Polynucleotide phosporylase can participate in decay of mRNA inEscherichia coli in the absence of ribonuclease II. Molec. gen. Genet.139, 357–362 (1975)
Kinscherf, T.G., Lee, Y.F., Apirion, D.: A technique for the iselation of mutants ofEscherichia coli affected in degradation of cellular RNA. Nucleic acid Research1, 1439–1453 (1974)
Krishna, R.V., Rosen, L., Apirion, D.: Increased inactivation and degradation of messenger ribonucleic acid in anEscherichia coli Strain containing a thermolarbile polynucleotide phosphorylase. Nature (Lond.) New Biol.242, 18–20 (1973)
Lennette, E.T., Gorelic, L., Apirion, D.: AnEscherichia coli mutant with increased messenger ribonuclease activity. Proc. nat. Acad. Sci. (Wash.)68, 3140–3144 (1971)
Reiner, A.M.: Characterization of polynucleotide phosphorylase mutants ofEscherichia coli. J. Bact.97, 1437–1443 (1969)
Robertson, H.D., Dunn, J.J.: Ribonucleic acid processing activity ofEscherichia coli ribonuclease III. J. biol. Chem.250, 3050–3056 (1975)
Robertson, H.D., Webster, R.E., Zinder, N.D.: Purification and properties of ribonuclease III fromEscherichia coli. J. biol. Chem.243, 82–91 (1968)
Schwartz, T., Craig, E., Kennell, D.: Inactivation and degradation of messenger ribonucleicacid from the lactose operon ofEscherichia coli. J. molec. Biol.54, 299–311 (1970)
Schweitz, H., Ebel, J.P.: A study of the mechanism of action ofE. coli ribonuclease III. Biochimie53, 585–593 (1971)
Simon, M., Apirion, D.: Increased inactivation of messengers inEscherichia coli ts mutant Nature (Lond.) New Biol.239, 79–81 (1972)
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Communicated by F. Kaudewitz
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Apirion, D., Neil, J. & Watson, N. Consequences of losing ribonuclease III on theEscherichia coli cell. Molec. Gen. Genet. 144, 185–190 (1976). https://doi.org/10.1007/BF02428107
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DOI: https://doi.org/10.1007/BF02428107