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Somatic hybridization experiments in solanaceous species

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Summary

Protoplasts were isolated from leaves of shoot cultures ofNicotiana tabacum var. Xanthi andPetunia hybrida, nuclei fromPetunia protoplasts and subprotoplasts from the liquid part of tomato pericarp. They were submitted in several combinations to agglutination by polyethyleneglycol (Kao and Michayluk, 1974) and to fusogenic treatment at pH 9 (Keller and Melchers, 1973) in calcium nitrate solution (Binding, 1974a; Schieder, 1974a). The fate of heterospecific symplasms was investigated during subsequent culture in medium NT-A (Nagata-Takebe medium, modification A: Binding, 1975) and in medium V47 (Binding, 1974b). Cell divisions occurred in tobacco +Petunia andPetunia + tomato symplasms which contained only a few tomato chromoplasts. Incompatibility is supposed to be responsible for the failure of divisions in tobacco + tomato symplasms and inPetunia + tomato symplasms containing a large tomato subprotoplast. The advantage of subprotoplasts for hybridization experiments is discussed in comparison to protoplast fusion and organell transplantation.

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Authors and Affiliations

  1. Botanisches Institut der Universität Kiel, Düsternbrooker Weg 17, D-2300, Kiel, Federal Republic of Germany

    Horst Binding

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  1. Horst Binding
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Communicated by G. Melchers

The experiments have been carried out in the Max-Planck-Institut für Züchtungsforschung (Erwin-Bauer-Institut), Abteilung Straub, in Köln-Vogelsang.

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Binding, H. Somatic hybridization experiments in solanaceous species. Molec. Gen. Genet. 144, 171–175 (1976). https://doi.org/10.1007/BF02428105

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  • DOI: https://doi.org/10.1007/BF02428105

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