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Granulocyte chemiluminescence response to serum opsonized zymosan particlesex vivo during long-term strenuous exercise, energy and sleep deprivation in humans

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Abstract

The chemiluminescence response of granulocytes to serum opsonized zymosan particles (SOZ) ex vivo was investigated during two ranger training courses lasting 7 days with continuous moderate physical activities corresponding to about 32% of maximal oxygen uptake or 35 000 kJ · 24 h−1, with energy deficiency (energy supply 0-4000 kJ · 24 h−1), and less than 3-h sleep during the 7 days. Significant granulocytosis in combination with a lymphopenia in peripheral blood was observed during the whole course. A priming of the granulocytes for accentuated chemiluminescence response to SOZ was observed during the first days of the course with a maximal increase on day 3 in course A (+35% of control response) and on day 1 in course B (+ 12%). Thereafter, reduced responses to SOZ compared to control values (−28% and −21% in course A and B) were observed. In course A, a group (n = 8) receiving 5000 kJ · 24 h−1 of additional energy, showed a more pronounced priming (maximum +57% versus +21 % of control response) during the first days. In course B, all the cadets had 3 h of organised rest/sleep on day 5, and a second priming of the chemiluminescence response was observed on the subsequent 2 days. These data indicated that moderate, continuous, predominantly aerobic physical activities for 1–3 days around the clock primed the production of reactive oxygen species in granulocytes. This priming may be beneficial for, for example, host defence against micro-organisms, but may also contribute to inflammatory damage to normal tissues such as muscle, tendons and joints during exercise. However, when the moderate exercise continued for several more days, a down-modulation of the granulocyte response was observed. The findings of this study further support the possibility that moderate physical activity stimulates immunity, while more extreme duration of the same activities may result in a down-modulation of nonspecific (and specific) immunity.

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References

  • Al-Mohanna FA, Haller MB (1992) Mechanisms of oxidase activation in neutrophils.In: Harris JR (ed) Blood cell biochemistry, 3. Lymphocytes and granulocytes. Plenum Press, New York, pp 289–334

    Google Scholar 

  • Armstrong RB, Warren GL, Warren JA (1991) Mechanisms of exercise-induced muscle fibre injury. Sports Med 12:184–207

    PubMed  CAS  Google Scholar 

  • Babior BM (1984) Oxidants from phagocytes: agents of defence and destruction. Blood 64:959–966

    PubMed  CAS  Google Scholar 

  • Bernton E, Galloway R, Hoover D (1994). Immune function II: monocyte and granulocyte function. Nutritional and imunological assessment of ranger students with increased caloric intake, chapter 10, Report No T95-5. US Army Research Institute of Environmental Medicine, Natick, Mass.

    Google Scholar 

  • Bishop CR, Athens JW, Boggs DR, Warner HR, Cartwright GE, Wintrobe MM (1968) Leukokinetic studies. XIII. A non-steadystate kinetic evaluation of the mechanism of cortisone-induced granulocytosis. J Clin Invest 47:249–260

    PubMed  CAS  Google Scholar 

  • Bosenberg AT, Brock-Utne JG, Gaffin SL, Wells B, Blake TW (1988) Strenuous exercise causes systemic endotoxemia. J Appl Physiol 65:106–108

    PubMed  CAS  Google Scholar 

  • Bøyum A (1968) Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Invest 21 [Suppl 97]:77–89

    Google Scholar 

  • Bøyum A, Wiik P, Gustavsson E, Veiby OP, Reseland J, Haugen A-H, Opstad PK (1996) The effect of strenuous exercise, calorie deficiency and sleep deprivation on white blood cells, plasma immunoglobulins and cytokines. Scand J Immunol 43:228–235

    Article  PubMed  Google Scholar 

  • Broch-Utne JG, Gaffin SL, Wells MT, Gathiram P, Sohar E, James ME, Morrell DF, Norman RJ (1988) Endotoxinemia in exhausted runners after a long-distance race. S Aft Med J 73:533–536

    Google Scholar 

  • Dahlgren C, Follin F, Johanson A, Lock R, Lundqvist H, Walan Å (1991) Chemiluminescence as a means of following the function of phagocytic cells. Trends Photochem Photobiol 2:427–443

    CAS  Google Scholar 

  • Fitzgerald L (1988) Exercise and the immune system. Immunol Today 9:337–339

    Article  PubMed  CAS  Google Scholar 

  • Foster LB, Dunn RT (1974) Single-antibody technique for radioimmunoassay of cortisol in unextracted serum or plasma. Clin Chem 20:365–368

    PubMed  CAS  Google Scholar 

  • Fuchs D, Malkovsky M, Reibnegger G, Werner ER, Forni G, Wachter H (1990) Endogenous release of interferon-gamma and diminished response of peripheral blood mononuclear cells to antigenic stimulation. Immunol Lett 23:103–108

    Article  Google Scholar 

  • Gabriel H, Urhausen A, Kindermann W (1992) Mobilization of circulating leukocyte and lymphocyte subpopulations during and after short, anaerobic exercise. Eur J Appl Physiol 65:164–170

    Article  CAS  Google Scholar 

  • Ganong WF (1991) Circulating body fluids.In: Ganong (ed) Review of medical physiology. Appleton and Lange, Colo., USA, pp 479–483

    Google Scholar 

  • Gibson-Berry KL, Whitin JC, Cohen HJ (1993) Modulation of the respiratory burst in human neutrophils by isoproterenol and dibutyryl cyclic AMP. J Neuroimmunol 43:59–68

    Article  PubMed  CAS  Google Scholar 

  • Gray AB, Telford RD, Collins M, Baker MS, Weidemann MJ (1993) Granulocyte activation induced by intense interval running. J Leukocyte Biol 53:591–597

    PubMed  CAS  Google Scholar 

  • Green RJ, Kaplan SS, Rabin BS, Stanitski CL, Zdziarski U (1981) Immune function in the marathon runner. Ann Allergy 47:73–75

    PubMed  CAS  Google Scholar 

  • Hack V, Strobel G, Rau JP, Weicker H (1992) The effect of maximal exercise on the activity of neutrophil granulocytes. Eur J Appl Physiol 65:520–524

    Article  CAS  Google Scholar 

  • Heijnen CJ, Kavelaars A, Ballieux RY (1991) Corticotropin-releasing hormone and pro opiomelanocortin-derived peptides in the modulation of immune function.In: Ader R, Felten DL, Cohen N (eds) Psychoneuroimmunology. Academic Press, New York, pp 429–444

    Google Scholar 

  • Hoffman-Goetz L, Pedersen B K (1994) Exercise and the immune system: a model of the stress response? Immunol Today 8:382–387

    Article  Google Scholar 

  • Huizinga TWJ, de Haas M, Kleijer M, Nuijens JH, Roos D, von der Borne A (1990) Soluble Fcγ receptor III in human plasma originates from release by neutrophils. J Clin Invest 86:416–423

    Article  PubMed  CAS  Google Scholar 

  • Keast D, Kameron K, Morton AR (1988) Exercise and the immune response. Sports Med 5:248–267

    PubMed  CAS  Google Scholar 

  • Khansari DN, Murgo AJ, Faith RE (1990) Effects of stress on the immune system. Immunol Today 11:170–175

    Article  PubMed  CAS  Google Scholar 

  • Kiecolt-Glaser JK, Glaser R (1991) Stress and immune function in humans.In: Ader R, Felten DL, Cohen N (eds) Psychoneuroimmunology. Academic Press, New York, pp 849–864.

    Google Scholar 

  • Kokot K, Schaefer RM, Teschner M, Gilge U, Plass R, Heidland A (1988) Activation of leukocytes during prolonged physical exercise. Adv Exp Med Biol 240:57–63

    PubMed  CAS  Google Scholar 

  • Kramer T (1994) Immune function I: lymphocyte proliferation & subset analysis; interleukin production. Nutritional and immunological assessment of ranger students with increased caloric intake, chapter 9, Report No T95-5. US Army Research Institute of Environmental Medicine, Natick, Mass.

    Google Scholar 

  • Lewicki R, Tchórzewski H, Denys A, Kowalska M, Golinska A (1987) Effects of physical exercise on some parameters of immunity in conditioned sportsmen. Int J Sports Med 8:309–314

    PubMed  CAS  Google Scholar 

  • Loeper M, Crouzon O (1904) L'action de l'adrénalin sur le sang. Arch Med Exp Anat Pathol 18:83–108

    Google Scholar 

  • Malec P, Tchórzewski H, Markiewicz K, Zeman K, Baj Z, Nowak Z, Pokoca L (1989) Some mechanisms of immunosuppressive action of epinephrine in humans. Allergol Immunopathol 117:81–84

    Google Scholar 

  • McGillis JP, Mitsuhashi M, Payan DG (1991) Immunological properties of substance P.In: Ader R, Felten DL, Cohen N (eds) Psychoneuroimmunology. Academic Press, New York, pp 209–220

    Google Scholar 

  • Øktedalen O, Lunde OC, Opstad PK, Aabakken L, Kvernebo K (1992) Changes in the gastrointestinal mucosa after long-distance running. Scand J Gastroenterol 27:270–274

    PubMed  Google Scholar 

  • Opstad PK (1990) Adrenergic desensitization and alterations in free and conjugated catecholamines during prolonged strain, sleep and energy deficiency. Biogen Amines 7:625–639

    CAS  Google Scholar 

  • Opstad PK (1991) Alterations in the morning plasma levels of hormones and the endocrine responses to bicycle exercise during prolonged strain. The significance of energy and sleep deprivation. Acta Endocrinol 125:14–22

    PubMed  CAS  Google Scholar 

  • Ottaway CA (1991) Vasoactive intestinal peptide and immune function.In: Ader R, Felten DL, Cohen N (eds) Psychoneuroimmunology. Academic Press, New York, pp 225–256

    Google Scholar 

  • Sharp NC, Koutedakis Y (1992) Sport and the overtraining syndrome: Immunological aspects. Br Med Bull 48:518–533

    PubMed  CAS  Google Scholar 

  • Smith JA, Telford RD, Mason IB, Weidemann MJ (1990) Exercise, training and neutrophil microbicidal activity. Int J Sports Med 11:179–187

    PubMed  CAS  Google Scholar 

  • Tilz GP, Domej W, Diez-Ruiz A, Weiss G, Brezinschek R, Brezinschek HP, Hüttl E, Pristautz H, Wachter H, Fuchs D (1993) Increased immune activation during and after physical exercise. Immunobiology 188:194–202

    PubMed  CAS  Google Scholar 

  • Weicker H, Werle E (1991) Interaction between hormones and the immune system. Int J Sports Med 12 [Suppl 1]:S30-S37

    Article  PubMed  Google Scholar 

  • Wiik P (1989) Vasoactive intestinal peptide inhibits the respiratory burst in human monocytes by a cyclic AMP-mediated mechanism. Regul Pept 25:187–197

    Article  PubMed  CAS  Google Scholar 

  • Wiik P, Skrede KK, Knardahl S, Haugen A-H, Ærø CE, Opstad PK, Boyum A (1995) Effect of in vivo corticosterone and acute food deprivation on rat resident peritoneal cell chemiluminescence after activation ex vivo. Acta Physiol Scand 154:407–416

    Article  PubMed  CAS  Google Scholar 

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  1. Norwegian Defence Research Establishment, Division for Environmental Toxicology, P.O. Box 25, N-2007, Kjeller, Norway

    P. Wiik, P. K. Opstad & A. Bøyum

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  1. P. Wiik
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  2. P. K. Opstad
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  3. A. Bøyum
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Wiik, P., Opstad, P.K. & Bøyum, A. Granulocyte chemiluminescence response to serum opsonized zymosan particlesex vivo during long-term strenuous exercise, energy and sleep deprivation in humans. Europ. J. Appl. Physiol. 73, 251–258 (1996). https://doi.org/10.1007/BF02425484

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