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Production, identification, and standardization of insect viral pathogens

  • International Symposium on the Identification and Assay of Viruses and Bacillus Thuringiensis Used for Insect Control (London, 13th July 1964)
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Résumé

Avant de considérer un virus d'insecte comme potentiellement utilisable en lutte microbiologique, on doit tenir compte non seulement de son identification et de sa standardisation mais aussi de la méthode de production. La pureté et la qualité des préparations de virus sont avant tout une fonction de sa méthode de production. Parmi les méthodes disponibles, la production de virus par multiplication sur insectes élevés sur milieux artificiels, offre présentement la meilleure voie pour obtenir un produit de qualité.

Les virus d'insectes peuvent être identifiés par leurs caractères physiques, morphologiques, chimiques aussi bien que par les tests sérologiques et de spécificité vis-à-vis des hôtes. Cependant, aucune de ces méthodes prises isolément n'est adéquate. Dans l'état actuel des connaissances, l'utilisation conjointe des deux derniers tests fournit les meilleurs éléments pour l'identification spécifique d'un virus d'insecte.

La standardisation d'un virus devrait être basée à la fois sur sa capacité à produire la maladie et sur le dénombrement des corps d'inclusion. La variation constatée dans la mesure de l'activité virale peut être une conséquence de la présence de particules de virus libérées, du nombre de particules virales dans les corps d'inclusion, de la taille et de la constitution chimique de ceux-ci ou de la présence de biotypes secondaires. Diverses techniques, le plus souvent basées sur des études de dose léthale et de temps de mortalité, peuvent être employées pour l'estimation de l'activité insecticide des préparations de virus. Quelle que soit la technique utiliséc, il est nécessaire de déterminer aussi précisément que possible, les paramètres de la méthode de titrage et d'estimer et de présenter statistiquement les résultats.

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  1. C. M. Ignoffo
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Ignoffo, C.M. Production, identification, and standardization of insect viral pathogens. Entomophaga 10, 29–40 (1965). https://doi.org/10.1007/BF02378782

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