Skip to main content
SpringerLink
Log in

Comparison of 13 published cytochrome c oxidase near-infrared spectroscopy algorithms

  • Original Article
  • Published:
European Journal of Applied Physiology and Occupational Physiology Aims and scope Submit manuscript

Abstract

Conflicting patterns of change in cytochrome c oxidase (Cyt a,a3) redox status have been obtained between different near-infrared spectrophotometers when making measurements during tissue ischaemia. This study identifies possible sources of error that could be the cause of the discrepancy. A single set of optical density data was repeatedly analysed using each of the absorption spectra from 13 publications. In addition, changes in Cyt a,a3 redox status were calculated from the data set using three numerical methods, five computer software routines, eight displacements in wavelength, and ten incremental changes in the value of absorption or concentration coefficients. All Cyt a,a3 absorption spectra resulted in algorithms yielding similar patterns of change, regardless of the numerical method or computer process employed (0.9996 averager 2, coefficient of correlation). However, a significantly different pattern of change in Cyt a,a3 redox status, resembling that reported by Piantadosi [Piantadosi CA (1993) Methods Toxicol. 2:107–126], was obtained when either the wavelengths, and/or the absorption values were altered to simulate erroneous values. This implies that all of the present algorithms are valid (including those of Piantadosi), but that microchip encoding errors may exist in the instrument used by Piantadosi.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benesch RE, Benesch R, Yung S (1973) Equations for the spectrophotometric analysis of hemoglobin mixtures. Anal Biochem 55:245–248

    Article  CAS  PubMed  Google Scholar 

  • Brazy JE (1990) Near infrared spectrophotometry. In: Nelson NM (ed) Current therapy in neonatal-perinatal medicine, vol 2. Decker, Toronto, pp 439–444

    Google Scholar 

  • Brunori M, Antonini E, Wilson MT (1981) Cytochrome c oxidase: an overview of recent work. In: Siegel H (ed) Metal ions in biological systems, XXIII. Dekker, New York, pp 187–228

    Google Scholar 

  • Cope M (1991) The development of a near infrared spectroscopy system and its application for non-invasive monitoring of cerebral blood and tissue oxygenation in the newborn infant. Thesis, Dept of Medical Physics and Bioengineering, University College London, pp 263–269

    Google Scholar 

  • Cope M, Delpy DT (1988) A system for long term measurement of cerebral blood and tissue oxygenation in newborn infants by near infrared transillumination. Med Biol Eng Comput 26:289–294

    CAS  PubMed  Google Scholar 

  • Cope M, Delpy DT, Reynolds EOR, Wray S, Wyatt J, van der Zee P (1988) Methods of quantitating cerebral near infrared spectroscopy data. In: Mochizuki M, Honig CR, Koyama T (eds) Oxygen transport to tissue X. Plenum, New York, pp 183–189

    Google Scholar 

  • Delpy DT, Cope MC, Cady EB, Wyatt JS, Hamilton PA, Hope PL, Wray S, Reynolds EOR (1987) Cerebral monitoring in newborn infants by magnetic resonance and near infrared spectroscopy. Scand J Clin Lab Invest 47:9–17

    Google Scholar 

  • Duhaylongsod FG, Griebel JA, Bacon DS, Wolfe WG, Piantadosi CA (1993) Effects of muscle contraction on cytochrome a,a3 redox state. J Appl Physiol 75:790–797

    CAS  PubMed  Google Scholar 

  • Edwards AD, Richardson C, van der Zee P, Elwell C, Wyatt JS, Cope M, Delpy DT, Reynolds EOR (1993) Measurement of hemoglobin flow and blood flow by near-infrared spectroscopy. J Appl Physiol 75:1884–1889

    CAS  PubMed  Google Scholar 

  • Elwell CE, Owen-Reece H, Cope M, Wyatt JS, Edwards AD, Delpy DT, Reynolds EOR (1993) Measurement of adult cerebral haemodynamics using near infrared spectroscopy. Acta Neurochir 59:74–80

    CAS  Google Scholar 

  • Greenwood C, Wilson MR, Brunori M (1974) Studies on partially reduced mammalian cytochrome oxidase. Biochem J 137:205–215

    CAS  PubMed  Google Scholar 

  • Griffiths DE, Wharton DC (1961) Studies on the electron transport system. XXXV. Purification and properties of cytochrome oxidase. J Biol Chem 236:1850–1856

    CAS  PubMed  Google Scholar 

  • Hampson NB, Piantadosi CA (1988) Near infrared monitoring of human skeletal muscle oxygenation during forearm ischemia. J Appl Physiol 64:2249–2257

    Google Scholar 

  • Hasegawa M, Sadataka H, Takashima S, Tatsuno M, Okuyama K, Suzuki S (1992) Monitoring of immature rabbit brain during hypoxia with near-infrared spectroscopy. Peditatr Neurol 8:47–50

    CAS  Google Scholar 

  • Hoffmann J, Heinrich U, Ahmad HR, Lubbers DW (1985) Analysis of tissue reflection spectra obtained from brain or heart, using the two flux theory for non-constant light scattering. Adv Exp Med Biol 191:555–563

    Google Scholar 

  • Jobsis FF (1977) Non-invasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198:1264–1267

    CAS  PubMed  Google Scholar 

  • Jobsis FF, Keizer JH, LaManna JC, Rosenthal M (1977) Reflectance spectrophotometry of cytochrome aa3 in vivo. J Appl Physiol 43:858–872

    CAS  PubMed  Google Scholar 

  • Jobsis-VanderVliet FF, Piantadosi CA, Sylvia AL, Lucas SK, Keizer HH (1988) Near-infrared monitoring of cerebral oxygen sufficiency 1. Spectra of cytochrome c oxidase. Neurol Res 10:7–17

    CAS  PubMed  Google Scholar 

  • Keizer HH, Jobsis-VanderVliet FF, Lucas SK, Piantadosi CA, Sylvia AL (1985) The near infrared absorption band of cytochrome a,a3 in purified enzyme, isolated mitochondria and in the intact brain in situ, Adv Exp Med Biol 191:823–832

    CAS  PubMed  Google Scholar 

  • Kitai T, Tanaka A, Tokuka A, Tanaka K, Yamaoda Y, Ozawa K, Hirao K (1993) Changes in the hepatic oxygenation state during hemorrhage and following epinephrine or dextran infusion as assessed by near-infrared spectroscopy. Circ Shock 41:197–205

    CAS  PubMed  Google Scholar 

  • Kline RA, Negendank WG, McCoy LE, Lester M, Berguer R (1990) MRI quantitation of edema in focal cerebral ischemia in cats: correlation with cytochrome aa3 oxidation state. Magn Reson Med 13:319–323

    CAS  PubMed  Google Scholar 

  • Lanczos C (1988) Applied analysis. Dover, New York, pp 118–122

    Google Scholar 

  • Macnab AJ, Gagnon R (1995) Near-infrared spectrophotometry data collection faults due to fiberoptic failure. Biomed Instrum Technol 29:405–409

    CAS  PubMed  Google Scholar 

  • Macnab AJ, Minchinton AI, Gagnon RF, Fryer KH, Seear M, Roland EH, Hill A (1994) Comparison of forearm oxygenation during ischaemia by near infrared spectroscopy and PO2 histography. Pediatr Res 36:26A

    Google Scholar 

  • Macnab AJ, Leblanc J, Gagnon F, Gagnon RE (1995) Near infrared spectroscopy oxidized cytochrome aa3 patterns of change during cardiac surgery in children. Pediatr Res 38:442

    Google Scholar 

  • Matcher SJ, Cope M, Delpy DT (1993) Use of the water absorption spectrum to quantify tissue chromophore concentration changes in near-infrared spectroscopy. Phys Med Biol 38:177–196

    Google Scholar 

  • Matcher SJ, Elwell CE, Cooper CE, Cope M, Delpey DT (1995) Performance comparison of several published tissue near-infrared spectroscopy algorithms. Anal Biochem 227:54–68

    Article  CAS  PubMed  Google Scholar 

  • Neter J, Wasserman W, Whitmore GA (eds) (1988) Applied statisties, 3rd edn. Allyn and Bacon, Newton, Mass, pp 604–610

    Google Scholar 

  • Parsons WJ, Rembert JC, Bauman RP, Greefield JC Jr, Piantadosi CA (1990) Dynamic mechanisms of cardiac oxygenation during brief ischemia and reperfusion. Am J Physiol 259:H1477-H1485

    CAS  PubMed  Google Scholar 

  • Parsons WJ, Rembert JC, Bauman RP, Duhaylongsod FG, Greenfield JC, Piantadosi CA (1993) Myocardial oxygenation in dogs during partial and complete coronary artery occlusion. Circ Res 73:458–464

    CAS  PubMed  Google Scholar 

  • Piantadosi CA (1993) Absorption spectroscopy for assessment of mitochondrial function in vivo. In: Lash H (ed) Methods in toxicology, vol 2. Academic Press, San Diego, pp 107–126

    Google Scholar 

  • Piantadosi CA, Comfort BJ (1989) Development and validation of multiwavelength algorithms for in vivo near infrared spectroscopy. In: Chance B (ed) Photon migration in tissue. Plenum, New York, pp 69–82

    Google Scholar 

  • Rea PA, Crowe J, Wickramasinghe Y, Rolfe P (1985) Non-invasive optical methods for the study of cerebral metabolism in the human newborn: a technique for the future? J Med Eng Technol 9:160–166

    CAS  PubMed  Google Scholar 

  • Stewart GW (1973) Introduction to matrix computations 1973. Academic-Press, New York, pp 221–224

    Google Scholar 

  • Thorniley MS, Livera LN, Wickramasinghe YABD, Spencer SA, Rolfe P (1990) The non-invasive monitoring of cerebral tissue oxygenation. In: Piper J (ed) Oxygen transport to tissue XII. Plenum, New York, pp 323–334

    Google Scholar 

  • Tuma J (1989) Handbook of numerical calculations in engineering. McGraw-Hill, New York, pp 144–156

    Google Scholar 

  • Wharton DC, Tzagoloff A (1964) Studies on the electron transfer system LVII: the near infrared absorption band of cytochrome oxidase. J Biol Chem 239:2036–2040

    CAS  PubMed  Google Scholar 

  • Wickramasinghe Y, Crowe J, Rolfe P (1986) Proceedings of the 8th Annual Conference on Engineering in Medicine and Biological Sciences. IEEE Service Center, Piscataway, NJ, pp 1172–1174

    Google Scholar 

  • Wolbarsht ML, Walsh AW, George G (1981) Melanin, a unique biological absorber. Appl Optics 20:2184–2186

    Google Scholar 

  • Wray S, Cope M, Delpy DT, Wyatt JS, Reynolds EOR (1988) Characterisation of the near infrared absorption spectra of cytochrome aa3 and haemoglobin for the non invasive monitoring of cerebral oxygenation. Biochim Biophys Acta 933:184–192

    CAS  PubMed  Google Scholar 

  • Wyatt JS, Delpy DT, Cope M, Wray S, Reynolds EOR (1986) Quantification of cerebral oxygenation and haemodynamics in sick newborn infants by near infrared spectrophotometry. Lancet II:1063–1066

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of British Columbia, BC Children's Hospital, 4480 Oak Street, V6H 3V4, Vancouver, British Columbia, Canada

    Roy E. Gagnon, Faith A. Gagnon, Andrew J. Macnab, R. E. Gagnon, F. A. Gagnon, A. J. Macnab & A. J. Macnab

Authors
  1. Roy E. Gagnon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Faith A. Gagnon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrew J. Macnab
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. E. Gagnon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. A. Gagnon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. J. Macnab
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. J. Macnab
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gagnon, R.E., Gagnon, F.A., Macnab, A.J. et al. Comparison of 13 published cytochrome c oxidase near-infrared spectroscopy algorithms. Europ. J. Appl. Physiol. 74, 487–495 (1996). https://doi.org/10.1007/BF02376763

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02376763

Key words

Search

Navigation