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Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea

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Abstract

Ten cDNAs for drought-inducible genes were isolated using differential screening of a cDNA library prepared from 10-hr dehydrated cowpea plants,Vigna unguiculata (S. Iuchi, K. Yamaguchi-Shinozaki, T. Urao, T. Terao, K. Shinozaki; Plant Cell Physiology, 1996 in press). Two of the cDNA clones, designated CPRD12 and CPRD46, were sequenced and characterized. The CPRD12 and CPRD46 cDNAs encode putative proteins related to nonmetallo-short-chain alcohol dehydrogenase (CPRD12) and chloroplastic lipoxygenase (CPRD46). Northern blot analysis revealed that these genes are induced by high-salinity stress and exogenous abscisic acid, but not by cold stress. The CPRD46 gene is also responsive to heat stress and methyl jasmonate and salicylic acid. Genomic Southern blot analysis suggested that CPRD12 constitutes a small gene family, but that CPRD46 is a single copy gene. We discuss the possible functions of these two CPRD gene products under drought stress.

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Abbreviations

ABA:

abscisic acid

ADH:

alcohol dehydrogenase

JA:

jasmonic acid

meJA:

methyl JA

SA:

salicylic acid

References

  • Bell, E., Creelman, R.A. andMullet, J.E. 1995. A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis. Proc. Natl. Acad. Sci. USA92: 8675–8679.

    CAS  PubMed  Google Scholar 

  • Bell, E. andMullet, J.E. 1993. Characterization of an Arabidopsis lipoxygenase gene responsive to methyl jasmonate and wounding. Plant Physiol.103: 1133–1137.

    Article  CAS  PubMed  Google Scholar 

  • Blechert, S., Brodschelm, W., Holder, S., Kammerer, L., Kutchan, T.M., Mueller, M.J., Xia, Z.Q. andZenk, M.H. 1995. The octadecanoic pathway: signal molecules for the regulation of secondary pathways. Proc. Natl. Acad. Sci. USA92: 4099–4105.

    CAS  PubMed  Google Scholar 

  • Bohnert, H.J., Nelson, D.E. andJensen, R.G. 1995. Adaptations to environmental stresses. Plant Cell7: 1099–1111.

    Article  CAS  PubMed  Google Scholar 

  • Bruxelles, G.L. de, Peacock, W.J., Dennis, E.S. andDolferus, R. 1996. Abscisic acid induces the alcohol dehydrogenase gene inArabidopsis. Plant Physiol.111: 381–391.

    PubMed  Google Scholar 

  • DeLong, A., Calderon-Urrea, A. andDellaporta, S.L. 1993. Sex determination geneTASSELSEED2 of maize encodes a short-chain alcohol dehydrogenase required for stage-specific floral organ abortion. Cell74: 757–768.

    Article  CAS  PubMed  Google Scholar 

  • Dolferus, R., Jacobs, M., Peacock, W.J. andDennis, E.S. 1994. Differential interactions of promoter elements in stress responses of theArabidopsis Adh gene. Plant Physiol.105: 1075–1087.

    Article  CAS  PubMed  Google Scholar 

  • Ealing, P.M. andCasey, R. 1988. The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA. Biochem. J.253: 915–918.

    CAS  PubMed  Google Scholar 

  • Ealing, P.M. andCasey, R. 1989. The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms. Biochem. J.264: 929–932.

    CAS  PubMed  Google Scholar 

  • Eiben, H.G. andSlusarenko, A.J. 1994. Complex spatial and temporal expression of lipoxygenase genes duringPhaseolus vulgaris (L.) development. Plant J.5: 123–135.

    CAS  PubMed  Google Scholar 

  • Enyedi, A.J., Yalpani, N., Silverman, P. andRaskin, I. 1992. Signal molecules in systemic plant resistance to pathogens and pests. Cell70: 879–886.

    Article  CAS  PubMed  Google Scholar 

  • Heijne, G. von andNishikawa, K. 1991. Chloroplast transit peptides. The perfect random coil? FEBS Lett.278: 1–3.

    Google Scholar 

  • Ingram, J. andBartels, D. 1996. The molecular basis of dehydration tolerance in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol.47: 377–403.

    Article  CAS  PubMed  Google Scholar 

  • Iuchi, S., Yamaguchi-Shinozaki, K., Urao, T., Terao, T. and Shinozaki, K. 1996. Novel drought-inducible genes in the highly drought-tolerant cowpea: cloning of cDNAs and analysis of their gene expression. Plant Cell Physiol. in press.

  • Jacobsen, S.E. andOlszewski, N.E. 1996. Gibberellins regulate the abundance of RNAs with sequence similarity to proteinase inhibitors, dioxygenases and dehydrogenases. Planta198: 78–86.

    Article  CAS  PubMed  Google Scholar 

  • Jany, K.-D., Ulmer, W., Froeschie, M. andPfleiderer, G. 1984. Complete amino acid sequence of glucose dehydrogenase fromBacillus megaterium. FEBS Lett.165: 6–10.

    Article  CAS  PubMed  Google Scholar 

  • Kawaoka, A., Kawamoto, T., Sekine, M., Yoshida, K., Takano, M. andShinmyo, A. 1994. A cis-acting element and a trans-acting factor involved in the wound-induced expression of a horseradish peroxidase gene. Plant J.6: 87–97.

    Article  CAS  PubMed  Google Scholar 

  • Malamy, J., Carr, J.P., Klessig, D.F. andRaskin, I. 1990. Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science250: 1002.

    CAS  Google Scholar 

  • Meier, B.M., Shaw, N. andSlusarenko, A.J. 1993. Spatial and temporal accumulation of defense gene transcripts in bean (Phaseolus vulgaris) leaves in relation to bacteria-induced hypersensitive cell death. Mol. Plant Microbe Interact.6: 453–66.

    CAS  PubMed  Google Scholar 

  • Melan, M.A., Dong, X., Eudara, M.E., Davis, K.R., Ausubel, F.M. andPeterman, T.K. 1993. AnArabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiol.101: 441–450.

    Article  CAS  PubMed  Google Scholar 

  • Nagy, F., Kay, S.A. andChua, N.-H. 1988. Analysis of gene expression in transgenic plants.In: S.V., Gelvin and R.A. Schilperoort, eds., Plant Molecular Biology Manual, B4. pp. 1–29. Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  • Ohta, H., Shirano, Y., Tanaka, K., Morita, Y. andShibata, D. 1992. cDNA cloning of rice lipoxygenase L-2 and characterization using active enzyme expressed from the cDNA inEscherichia coli. Eur. J. Biochem.206: 331–336.

    CAS  PubMed  Google Scholar 

  • Peng, Y.L., Shirano, Y., Ohta, H., Hibino, T., Tanaka, K. andShibata, D. 1994. A novel lipoxygenase from rice. Primary structure and specific expression upon incompatible infection with rice blast fungus. J. Biol. Chem.269: 3755–3761.

    CAS  PubMed  Google Scholar 

  • Persson, B., Krook, M. andJornvall H. 1991. Characteristics of short-chain alcohol dehydrogenases and related enzymes. Eur. J. Biochem.200: 537–543.

    CAS  PubMed  Google Scholar 

  • Reinbothe, S., Mollenhauer, B. andReinbothe, C. 1994. JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens. Plant Cell6: 1197–1209.

    Article  CAS  PubMed  Google Scholar 

  • Sambrook, J., Fritsch, E.F. andManiatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Sembdner, G. andParthler, B. 1993. The biochemistry and the physiological and molecular actions of jasmonates. Annu. Rev. Plant Physiol. Plant Mol. Biol.44: 569–589.

    Article  CAS  Google Scholar 

  • Shibata, D. andAxelrod, B. 1995. Plant lipoxygenases. J. Lipid Mediators Cell Signalling12: 213–228.

    CAS  Google Scholar 

  • Shibata, D., Kato, T. andTanaka, K. 1991. Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene. Plant Mol. Biol.16: 353–359.

    Article  CAS  PubMed  Google Scholar 

  • Shibata, D., Slusarenko, A., Casey, R., Hildebrand, D. andBell, E. 1994. Lipoxygenase. Plant Mol. Biol. Rep.12: S41-S42.

    CAS  Google Scholar 

  • Shibata, D., Steczko, J., Dixon, J.E., Andrews, P.C., Hermodson, M. andAxelrod, B. 1988. Primary structure of soybean lipoxygenase L-2. J. Biol. Chem.263: 6816–6821.

    CAS  PubMed  Google Scholar 

  • Shibata, D., Steczko, J., Dixon, J.E., Hermodson, M., Yazdanparast, R. andAxelrod, B. 1987. Primary structure of soybean lipoxygenase-1. J. Biol. Chem.262: 10080–10085.

    CAS  PubMed  Google Scholar 

  • Shinozaki, K. andYamaguchi-Shinozaki, K. 1996. Molecular responses to drought and cold stress. Cur. Opinion Biotech.7: 161–167.

    CAS  Google Scholar 

  • Singh, B.B. 1993. The cowpea breeding section of the archival report (1988–1992) of the grain legume improvement program. International Institute of Tropical Agriculture, Ipadan, Nigeria.

    Google Scholar 

  • Steczko, J., Donoho, G.P., Clemens, J.C., Dixon, J.E. andAxelrod, B. 1992. Conserved histidine residues in soybean lipoxygenase: functional consequences of their replacement. Biochemistry31: 4053–4057.

    Article  CAS  PubMed  Google Scholar 

  • Summerfield, R.J., Pate, J.S., Roberts, E.H. andWien, H.C. 1985. The physiology of cowpeas.In S.R. Singh and K.O. Rachie, eds., Cowpea Research, Production and Utilization. pp. 65–101. John Wiley & Sons, Chichester, U.K.

    Google Scholar 

  • Yamaguchi-Shinozaki, K., Urao, T. andShinozaki, K. 1995. Regulation of genes that are induced by drought stress inArabidopsis thaliana. J. Plant Res.108: 127–136.

    Article  CAS  Google Scholar 

  • Yenofsky, R.L., Fine, M. andLiu, C. 1988. Isolation and characterization of a soybean (Glycine max) lipoxygenase-3 gene. Mol. Gen. Genet.211: 215–222.

    Article  CAS  Google Scholar 

  • Zhang, Y.Y., Radmark, O. andSamuelsson, B. 1992. Mutagenesis of some conserved residues in human 5-lipoxygenase: effects on enzyme activity. Proc. Natl. Acad. Sci. USA89: 485–489.

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Ministry of Agriculture, Forestry, and Fisheries, 2-1 Ohwashi, 305, Tsukuba, Ibaraki, Japan

    Kazuko Yamaguchi-Shinozaki & Takeshi Urao

  2. Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), Tsukuba Life Science Center, 3-1-1 Koyadai, 305, Tsukuba, Ibaraki, Japan

    Satoshi Iuchi & Kazuo Shinozaki

  3. Institute of Biological Science, Tsukuba University, Tennohdai, 305, Tsukuba, Ibaraki, Japan

    Satoshi Iuchi & Kazuo Shinozaki

Authors
  1. Satoshi Iuchi
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  2. Kazuko Yamaguchi-Shinozaki
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  3. Takeshi Urao
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  4. Kazuo Shinozaki
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Iuchi, S., Yamaguchi-Shinozaki, K., Urao, T. et al. Characterization of two cDNAs for novel drought-inducible genes in the highly drought-tolerant cowpea. J. Plant Res. 109, 415–424 (1996). https://doi.org/10.1007/BF02344557

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  • DOI: https://doi.org/10.1007/BF02344557

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