Summary
The purpose of this study was to measure serum creatine kinase (CK) activity and serum myoglobin (MG) concentrations in women after two unilateral isometric knee extension exercises. Forty maximal voluntary contractions (MVC) were held for 10 s, with either a 5 s (10∶5) or 20 s 10∶20 exercise (349.4±66.1 mU · ml−1) and 6 h and MG values were measured pre, 0, 3, 6, and 18 h post exercise. For CK, the highest post exercise values were observed at 6 h following the 10∶20 exercise (349.4±66.1 mU · ml−1) and 6 h following the 10∶5 exercise (194.1±18.6 mU · ml−1). For MG, the highest values were found 3 h after the 10∶20 exercise (148.9±61.7 ng · ml−1) and 6 h after the 10∶5 exercise (67.3±10.9 ng · ml−1). Serum CK and MG levels were significantly greater (p<0.01) after the 10∶20 exercise bout. The data demonstrate that CK and MG values for women increase significantly after isometric exercise. Since greater tension levels were maintained during the 10∶20 exercise it is hypothesized that increased serum CK and MG values after isometric exercise may be related to the tension generated by the contracting muscle.
Similar content being viewed by others
References
Brooke MH, Carroll JE, Davis JE, Hagberg JM (1979) The prolonged exercise test. Neurology 29:636–643
Byrnes WC, Clarkson PM, White JS, Hseih SS, Frykman PN, Maughan RJ (1985) Delayed onset muscle soreness following repeated bouts of downhill running. J Appl Physiol 59:710–715
Clarkson PM, Kroll W, Graves J, Record WA (1982) The relationship of serum creatine kinase, fiber type, and isometric exercise. Int J Sports Med 3:145–148
Clarkson PM, Litchfield P, Graves J, Kirwan J, Byrnes WC (1985) Serum creatine kinase activity following forearm flexion isometric exercise. Eur J Appl Physiol 53:368–371
Haralambie G, Senser L, Sierra-Chavez R (1981) Physiological and metabolic effects of a 25 km race in female athletes. Eur J Appl Physiol 47:123–131
Hughes RC, Park DC, Parsons ME, O'Brien MD (1971) Serum creatine kinase studies in the detection of carriers of Duchenne dystrophy. J Neurol Neurosurg Psychiat 34:527–530
Johnson RN, Lubbe WF, Mercer CJ, Sammel NL, Norris RM (1982) Serum myoglobin, creatine kinase and creatine kinase MB as mutually supportive indices of myocardial infarction and infarct size. Aust NZ J Med 12:160–165
Konagaya M, Takayanagi T, Konagaya Y, Sobue I (1982) The fluctuation of serum myoglobin levels in Duchenne muscular dystrophy and the carrier. J Neuol Sci 55:259–265
Munjal DD, McFadden JA, Matrix PA, Coffman KD, Cattaneo SM (1983) Changes in serum myoglobin, total creatine kinase, lactate dehydrogenase and creatine kinase MB levels in runners. Clin Biochem 16:195–199
Norregaard Hansen K, Bjerre-Knudsen J, Brodthagen U, Jordal R, Paulev PE (1982) Muscle cell leakage due to long distance training. Eur J Appl Physiol 48:177–188
Nuttal FQ, Jones B (1968) Creatine kinase and glutamic oxalacetic transaminase activity in serum: Kinetics of change with exercise and effect of physical conditioning. J Lab Clin Med 71:847–854
Pearce JMS, Pennington RJ, Walton JN (1964) Serum enzyme studies in muscle disease. Part I. Variations in serum creatine kinase activity in normal individuals. J Neurology Neurosurg Psychiat 27:1–4
Sabria M, Ruibal A, Rey C, Foz M, Domenech FM (1983) Influence of exercise on serum levels of myoglobin measured by radioimmunoassay. Eur J Nucl Med 8:159–161
Shumate JB, Brooke MH, Carroll JE, Davis JE (1979) Increased serum creatine kinase after exercise: A sex-linked phenomenon. Neurology 29:902–904
Sylven JCH, Bomfim V, Ivert T, Olin C (1982) Myocardial and non-myocardial release of myoglobin and creatine-kinase MB following cardiac operations with hypothermie potassium cardioplegia. Cardiovas Res 16:151–157
Szasz G, Gruber W, Bernt E (1976) Creatine kinase in serum: 1. Determination of optimum reaction conditions. Clin Chem 22:650–656
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kirwan, J.P., Clarkson, P.M., Graves, J.E. et al. Levels of serum creatine kinase and myoglobin in women after two isometric exercise conditions. Europ. J. Appl. Physiol. 55, 330–333 (1986). https://doi.org/10.1007/BF02343808
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02343808