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A rapid and specific enrichment procedure forHyphomicrobium spp.

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Abstract

An enrichment procedure for the isolation of stalked bacteria of the genusHyphomicrobium is described. The method is based on the use of an organic C1 compound as carbon and energy source for growth together with anaerobic incubation in the presence of nitrate as an electron acceptor. Optimal conditions for the growth of a number ofHyphomicrobium isolates have been investigated. Applying these conditions,Hyphomicrobium spp. have been enriched from a wide range of natural habitats within 1–2 weeks.

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Author notes

  1. Margaret M. Attwood

    Present address: Department of Microbiology, University of Sheffield, England

Authors and Affiliations

  1. Department of Microbiology, University of Groningen, Kerklaan 30, Haren (Gr.), the Netherlands

    Margaret M. Attwood & W. Harder

Authors
  1. Margaret M. Attwood
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  2. W. Harder
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Additional information

This work was supported by a Grant from the Medical Research Fund, University of Sheffield, England, to M.M.A.

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Attwood, M.M., Harder, W. A rapid and specific enrichment procedure forHyphomicrobium spp.. Antonie van Leeuwenhoek 38, 369–377 (1972). https://doi.org/10.1007/BF02328108

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