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Ectomycorrhizal colonization on black spruce and jack pine seedlings outplanted in reforestation sites

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Abstract

Six-week-old, mycorrhiza-free, bareroot jack pine and black spruce seedlings were outplanted in ten reforestation sites, situated between 45–48° latitude N and 69–74° longitude W, within the province of Quebec, representing diverse operational forestry disturbances and ecological conditions.

Two months after outplanting, root systems of black spruce seedlings had fewer mycorrhizae than those of jack pine seedlings. Ectomycorrhizal colonization on black spruce seedlings did not vary significantly with the reforestation site. Percent mycorrhizal colonization for these seedlings was positively correlated with seedling dry weight while with the jack pine seedlings, mycorrhizal colonization varied significantly with the outplanting site and there was no correlation between mycorrhizal formation and seedling dry weight.

Multiple linear regressions showed pH to be a determinant soil factor for mycorrhizal colonization for the two species. Drainage was the other influential factor affecting colonization of black spruce while organic matter accumulation was more important for jack pine.

Inoculation with selected ectomycorrhizal fungi could be more important for black spruce than for jack pine seedlings.

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References

  • Alvarez I F, Rowney D L and Cobb F W Jr 1979 Mycorrhizae and growth of white fir seedlings in mineral soil with and without organic layers in a California forest. Can. J. For. Res. 9, 311–315.

    Google Scholar 

  • Anderson U L and McLean R A 1974 Design of Experiments. Deker, New York, 418 p.

    Google Scholar 

  • Boyle C D, Robertson W J and Salonius P O 1987 Use of mycelial slurries of mycorrhizal fungi as inoculum for commercial tree-seedling nurseries. Can. J. For. Res. 17, 1480–1486.

    Google Scholar 

  • Brown R T and Mikola P 1974 The influence of fruticose soil lichens upon the mycorrhizal and seedling growth of forest trees. Acta Forestalia Fennica. 141, 1–22.

    Google Scholar 

  • Browning M H R and Whitney R D 1987 Root growth and ectomycorrhizal colonization of outplanted containerizedPinus banksiana. In Mycorrhizae in the Next Decade: Practical Applications and Research Priorities. Eds. D M Sylvia, L L Hung and J H Graham. p 86. University of Florida.

  • Danielson R M, Visser S and Parkinson D 1984 Production of ectomycorrhizae on container-grown jack pine seedlings. Can. J. For. Res. 14, 33–36

    Google Scholar 

  • Duncan D B 1955 Multiple range and multiple f-tests. Biometrics 11, 1–42.

    Google Scholar 

  • Environment Canada 1981 Canadian Climate Normals 1951–1980: Temperature and Precipitation. Atmospheric Environment Service, Ottawa, Canada.

    Google Scholar 

  • Ferrier R C and Alexander I J 1985 Persistence under field conditions of excised fine roots and mycorrhizas of spruce.In Ecological Interactions in Soil. Special publication number 4 of the British Ecological Society. Ed. A H Fitter. pp. 193–217. Blackwell Scientific Publications, London.

    Google Scholar 

  • Fogel R 1980 Mycorrhizae and nutrient cycling in natural forest ecocystems. New Phytol. 86, 199–212.

    CAS  Google Scholar 

  • Fortin C, Fortin J A, Gaulin G A, Jomphe N, Laberge G and Lemay S 1987 Inoculation ectomycorrhizienne de plants forestiers a l’echelle industrielle. Rapport du projet PE86-1, Ministère de l’énergie et ressources. Québec, QC.

    Google Scholar 

  • Frankland J C and Harrison A F 1985 Mycorrhizal infection ofBetula pendula andAcer pseudoplatanus: Relationships with seedling growth and soil factors. New Phytol 101, 133–151.

    Google Scholar 

  • Gagnon J, Langois C G and Fortin J A 1987 Growth of containerized jack pine seedlings inoculated with different ectomycorrhizal fungi under a controlled fertilization schedule. Can. J. For. Res. 17, 840–845.

    Google Scholar 

  • Harvey A E, Jurgensen M F, Larsen M J and Graham R T 1987 Relationships among soil microsite, ectomycorrhizae and natural conifer regeneration of old-growth forests in western Montana. Can. J. For. Res. 17, 58–62.

    Google Scholar 

  • Harvey A E, Larsen M J and Jurgensen M F 1980 Partial cut harvesting and ectomycorrhizae: Early effects in Douglas-firlarch forests of Western Montana. Can. J. For. Res. 10, 436–440.

    Google Scholar 

  • Harvey A E, Larsen M J and Jurgensen M F 1979 Comparative distribution of ectomycorrhizae in soils of three western Montana forest habitat types. For. Sci. 25, 350–358.

    Google Scholar 

  • Hung L L and Trappe J M 1983 Growth variation between and within species of ectomycorrhizal fungi in response to pHin vitro. Mycologia 75, 234–241.

    Google Scholar 

  • Kropp B R 1982 Formation of mycorrhizae on non-mycorrhizal western hemlock outplanted on rotten wood and mineral soil. For. Sci. 28, 706–710.

    Google Scholar 

  • Kropp B R and Fortin J A 1987 The incompatibility system and relative ectomycorrhizal performance of monokaryons ofLaccaria bicolor. Can. J. Bot. 66, 289–294.

    Google Scholar 

  • Marx D H, Cordell C E, Kenny D S, Mexal J G, Artman J D. Riffle J W and Molina R A 1984 Commercial vegetative inoculum ofPisolithus tinctorius and inoculation techniques for development of ectomycorrhizae on bare root tree seedlings. For. Sci. Monograph 25. 101p.

  • Marx D H, Hatch A B and Mendicino J F 1977 High soil fertility decreases sucrose content and susceptibility of loblolly pine roots to ectomycorrhizal infection toPisolithus tinctorius. Can. J. Bot. 55, 1569–1574.

    CAS  Google Scholar 

  • Marx D H and Zak B 1965 Effect of pH on mycorrhizal formation of slash pine in aseptic culture. For. Sci. 11, 66–75

    Google Scholar 

  • McAfee B J and Fortin J A 1986 Competitive interactions of ectomycorrhizal mycobionts under field conditions. Can. J. Bot. 64, 848–852.

    Google Scholar 

  • Parke J L, Linderman R G and Trappe J M Inoculum potential of ectomycorrhizal fungi in forest soils of southwest Oregon and northern California. For. Sci. 30, 300–304.

  • Parke J L, Linderman R G and Trappe J M 1983 Effects of forest litter on ectomycorrhizae development and growth of Douglas fir and western red cedar seedlings. Can. J. For. Res. 13, 666–671.

    Google Scholar 

  • Perry D A, Meyer M M, Eyeland D, Rose S L and Pilz D 1982 Seedling growth and mycorrhizal formation in clearcut and adjacent undisturbed soils in Montana: A greenhouse bioassay. For. Ecol. Manage. 4, 261–273.

    Article  Google Scholar 

  • Pilz D P and Perry D A 1984 Impact of clearcutting and slash burning on ectomycorrhizal associations of Douglas-fir seedlings. Can. J. For. Res. 14, 94–100.

    Google Scholar 

  • Plamondon A P, Ouellet D C and Déry G 1980 Effets de la scarification du site sur le micro-environment. Can. J. For. Res. 10, 476–482.

    Google Scholar 

  • Richard C and Fortin J A 1974 Distribution géographique écologique, physiologie, pathogénécité et sporulation duMycellium radicis atrovirens. Phytoprotection 55, 67–88.

    Google Scholar 

  • Robinson R K 1972 The production byCalluna vulgaris of a factor inhibitory to growth of some mycorrhizal fungi. J. Ecol. 60, 219–224.

    Google Scholar 

  • SAS Institute Inc. 1982 A User’s Guide: Statistics, 1982 Edition. Cary North Carolina. 584 p.

  • Schoenberger M M and Perry D A 1982 The effect of soil disturbance on growth and ectomycorrhizae of Douglas fir and western hemlock seedlings: A greenhouse bioassay. Can. J. For. Res. 12, 343–353.

    Google Scholar 

  • Shafer S R, Grand L F, Bruck R I and Heagle A S 1985 Formation of ectomycorrhizae onPinus taeda seedlings exposed to simulated acidic rain. Can. J. For. Res. 15, 66–71.

    Google Scholar 

  • Shriner D S 1978 Effects of simulated acidified rain on hostparasite interactions on plant diseases. Phytopathology 68, 213–218.

    CAS  Google Scholar 

  • Stephens M A 1974 EDF statistics for goodness of fit and some comparisons. J. Am. Statist. Assoc. 69, 730–737.

    Google Scholar 

  • Theodorou C and Bowen G D 1969 The influence of pH and nitrate on mycorrhizal associations onPinus radiata. D. Don. Aust. J. Bot. 17, 59–67.

    Article  Google Scholar 

  • Truog E 1930 Determination of the readily available phosphorus of soils. J. Am. Soc. Agron. 22, 874–882.

    CAS  Google Scholar 

  • Tyler G 1985 Macrofungal flora of Swedish beech forest related to soil organic matter and acidity characteristics. For. Ecol. Manage. 10, 13–29.

    Article  Google Scholar 

  • Tyminska A, Letacon F and Chadoeuf J 1986 Effect of three ectomycorrhizal fungi on growth and phosphorus uptake ofPinus silvestris seedlings at increasing phosphorus levels. Can. J. Bot. 64, 2753–2757.

    CAS  Google Scholar 

  • Walkley A and Black I A 1934 An examination of the Degtjoreff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 37, 29–37.

    CAS  Google Scholar 

  • Wilson J, Mason P A, Last F T, Ingleby K and Munro R C 1987 Ectomycorrhizal formation and growth of Sitka spruce seedlings on first rotation forest sites in northern Britain. Can. J. For. Res. 17, 957–963.

    Google Scholar 

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  1. B. J. McAfee

    Present address: Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada

Authors and Affiliations

  1. Centre de Recherches en Biologie Forestières, Faculté de Foresterie et Géodésie, Université Laval, G1K 7P4, Ste-Foy, Québec, Canada

    B. J. McAfee & J. A. Fortin

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  1. B. J. McAfee
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  2. J. A. Fortin
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McAfee, B.J., Fortin, J.A. Ectomycorrhizal colonization on black spruce and jack pine seedlings outplanted in reforestation sites. Plant Soil 116, 9–17 (1989). https://doi.org/10.1007/BF02327251

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  • DOI: https://doi.org/10.1007/BF02327251

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