Abstract
Watermelon [Citrullus lanatus (Thunberg) Matsumura and Nakai] proliferating shoot meristems from established shoot cultures were inoculated on modified Murashige and Skoog salts medium supplemented with 10 μM 6-benzyladenine (BA) for shoot proliferation and on similar medium supplemented with 1 μM BA and 10 μM gibberellic acid (GA3) for shoot elongation. Agar-solidified medium and microporous polypropylene membrane rafts in liquid medium were used to support the tissues. Growth over culture time of proliferating and elongating tissues in liquid and agar-solidified media were compared. Nutrient depletion in liquid medium was monitored and quantified using ion selective electrodes. Tissue fresh weights in both proliferation and shoot elongation media were greater in liquid than in agar-solidified medium. Relative dry matter content, however, was greater in agar-solidified than in liquid medium. More shoots elongated in agar-solidified than in liquid medium. The numbers of buds or unelongated shoot meristems, however, were comparable for both the liquid and agar-solidified medium. Proliferating and elongating tissues in liquid medium used Ca++ and K+ minimally. NO −3 was utilized but not depleted by proliferating tissues. NH +4 , however, was depleted. Most of the NH +4 was utilized by the proliferating tissues within 21 days of culture when growth rate was greatest. At 35 days, residual Ca++, K+, NO −3 , and NH +4 in proliferation medium were 81.0%, 67.8%, 55.7%, and 1.2% of initial levels, respectively. NO −3 and NH +4 in shoot elongation medium were depleted. The greatest NO −3 and NH +4 utilization was observed during the first 14 days of culture when the largest growth rate was obtained. The residual Ca++, K+, NO −3 , and NH +4 in shoot elongation medium at 38 days were 63.5%, 37.9%, 21.2%, and 24.3% of initial concentrations, respectively. At the end of experiment, 72.3% and 42.8% of initial sugars were still remaining in the shoot proliferation and shoot elongation medium, respectively.
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References
Adelberg J, Desamero NV, Hale A & Young R (1992) Orchid micropropagation on polypropylene membranes (pp 688–695). Amer. Orchid Soc. Bul. July 1992
Debergh PC (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant. 59: 270–276
Debergh P, Harbaoui Y & Lemeur R (1981) Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol. Plant. 53: 181–187
Debergh P, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R & Ziv M (1992) Reconsideration of the term ‘vitrification’ as used in micropropagation. Plant Cell Tiss. Org. Cult. 30: 135–140
Dougall DK (1980) Nutrition and metabolism. In: Staba EJ (Ed) Plant Tissue Culture as a Source of Biochemicals (pp 21–58). CRC Press, Boca Raton, Florida
Dougall DK & Frazier GC (1989) Nutrient utilization during biomass and anthocyanin accumulation in suspension cultures of wild carrot cells. Plant Cell Tiss. Org. Cult. 18: 95–104
Hew CS, Ting SK & Chia TF (1988) Substrate utilization byDendbrobium tissues. Bot. Gaz. 149: 153–157
Hew CS, Chan YS, Lee YK & Chia TF (1990) Culture of orchid tissue on polypropylene membrane. Malayan Orchid Rev. 24: 78–81
Kohlenbach HW & Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z. Pflanzenphysiol. 86: 463–472
Lumsden PJ, Pryce S & Leifert C (1990) Effect of mineral nutrition on the growth and multiplication ofin vitro cultured plants. In Nijkamp HJJ, Van Der Plas LHW & Van Aartrijk J (Eds) Plant Cell and Molecular Biology (pp 108–113). Kluwer Academic Publishers, Dordrecht, The Netherlands
MacCarthy JJ, Ratcliffe D & Street HE (1980) The effect of nutrient medium composition on the growth cycle ofCatharanthus roseus G. Don cells grown in batch culture. J. Exp. Bot. 31: 1315–1325
McDonald KA & Jackman AP (1989) Bioreactor studies of growth and nutrient utilization in alfalfa suspension cultures. Plant Cell Rep. 8: 455–458
Martin SM & Rose D (1976) Growth of plant cell (Ipomoea) suspension cultures at controlled pH levels. Can. J. Bot. 54: 1264–1270
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Romberger JA & Tabor CA (1971) ThePicea abies shoot apical meristem in culture. I. Agar and autoclaving effects. Amer. J. Bot. 58: 131–140
Singha S, Townsend EC & Oberly GH (1985) Mineral nutrient status of crabapple and pear shoots culturedin vitro on varying concentrations of three commercial agars. J. Amer. Soc. Hort. Sci. 11: 407–411
Singha S, Oberly GH & Townsend EC (1987) Changes in nutrient composition and pH of the culture medium duringin vitro shoot proliferation of crabapple and pear. Plant Cell Tiss. Org. Cult. 11: 209–220
Stoltz LP (1971) Agar restriction of the growth of excised mature iris embryo. J. Amer. Soc. Hort. Sci. 96: 618–684
Vasil IK (1991) Rationale for the scale-up and automation of plant propagation. In: Vasil IK (Ed) Scale-Up and Automation in Plant Propagation. Cell Culture and Somatic Cell Genetics of Plants, Vol 8 (pp 1–6). Academic Press, Inc., New York, N.Y.
Young RE, Hale A, Camper ND, Keese RJ & Adelberg JW (1991) Approaching mechanization of plant micropropagation. Transactions of the ASAE 34: 328–333
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Technical Contribution No. 3236 of the South Carolina Agricultural Experiment Station.
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Desamero, N.V., Adelberg, J.W., Hale, A. et al. Nutrient utilization in liquid/membrane system for watermelon micropropagation. Plant Cell Tiss Organ Cult 33, 265–271 (1993). https://doi.org/10.1007/BF02319011
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DOI: https://doi.org/10.1007/BF02319011