Abstract
Experiments are described which improve the protocols for initiating in vitro cultures of sugarcane and allowing efficient regeneration of plants even after 30 months of callus proliferation. Procedures adopted included use of leaf base explants, CS medium with 3 mg/l 2, 4-D and 0.25 mg/l kinetin for callus initiation and growth, MS medium with 0.5 mg/l IAA and 1 mg/l BAP for shoots, MS medium with 5 mg/l NAA and 7% (wt/vol) sucrose for rooting of shoots. Casein hydrolysate (N-Z amine) significantly shortened the lag period in the growth of sugarcane suspension cultures, but did not increase the rate of growth following the lag phase. Protoplasts isolated from two types of cultures could be grown to re-establish cell cultures but no plants have yet been regenerated derived from isolated protoplasts.
Similar content being viewed by others
References
Barba R, Nickell LG (1969) Nutrition and organ differentiation in tissue cultures of sugarcane, a monocotyledon. Planta 89:299–302
Barba RC, Zamora AB, Mallion AK, Linga CK (1977) Sugarcane tissue culture research. Proc Int Soc Sug Cane Technol 15, Plant breeding section 1–8
Behki RM, Lesley SM (1980) Shoot regeneration from leaf callus ofLycopersicon esculentum. Z Pflanzenphysiol 98:83–87
Binding H (1974) Regeneration of haploid and diploid plants from protoplasts ofPetunia hybrida L. Z Pflanzenphysiol 74:327–356
Cai Q-G, Qian Y-Q, Zhou Y-L, Wu S-X (1978) A further study on the isolation and culture of rice (Oryza sativa L.) protoplasts. Acta Bot Sinica (Peking) 20:97–103
Deka PC, Sen SK (1976) Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique. Mol Gen Genet 145:239–243
Evans DA, Crocomo OJ, de Carvalho MT (1980) Protoplast isolation and subsequent callus regeneration in sugarcane. Z Pflanzenphysiol 98:355–358
Gamborg OL, Miller RA, Ojima K (1968). Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Gibson AH, Child JJ, Pagan JD, Scowcroft WR (1976) The induction of nitrogenase activity inRhizobium by non-legume plant cells. Planta 128:233–239
Heinz DJ (1973) Sugar-cane improvement through induced mutations using vegetative propagules and cell culture techniques. In: Induced mutations in vegetatively propagated plants (Proc of a panel, 11–15 Sept 1972). Vienna: International Atomic Energy Agency, pp 53–59
Heinz DJ, Mee GWP (1969) Plant differentiation from callus tissue ofSaccharum species. Crop Sci 9:346–348
Heinz DJ, Mee GWP (1971) Morphologic, cytogenetic, and enzymatic variation inSaccharum species hybrid clones derived from callus tissue. Am J Bot 58:257–262
Heinz DJ, Krishnamurthi M, Nickell LG, Maretzki A (1977) Cell tissue and organ culture in sugarcane improvement. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Berlin: Springer, pp 3–17
Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean —Nicotiana glauca. Mol Gen Genet 150:225–230
Krishnamurthi M (1976) Isolation, fusion and multiplication of sugarcane protoplasts and comparison of sexual and parasexual hybridization. Euphytica 25:145–150
Krishnamurthi M, Tlaskal J (1974) Fiji disease resistantSaccharum officinarum var. Pindar sub-clones from tissue cultures. Proc Int Soc Sug Cane Technol 15, Plant breeding section 1–8
Lat JB, Lantin MM (1976) Agronomic performance of sugarcane clones derived from callus tissue. Philipp J Crop Sci 1:117–123
Liu N-C, Chen W-H (1974) Histological studies on the origin and process of plantlet differentiation in sugarcane callus mass. Proc 15th Congress Int Soc Sug Cane Technol, Plant breeding section 118–128
Liu M-C, Chen W-H (1976) Tissue and cell cuture as aids to sugarcane breeding. I. Creation of genetic variation through callus culture. Euphytica 25:393–403
Liu M-C, Chen W-H (1976) Fusion and cell clump formation in sugarcane protoplasts. Sugarcane Breed Newslett 37:39–46
Liu M-C, Chen W-H (1978) Tissue and cell culture as aids to sugarcane breeding. II. Performance and yield potential of callus derived lines. Euphytica 27:273–282
Liu M-C, Chen W-H (1978) Improvement in sugarcane by using tissue culture methods. In: Fourth International Congress Plant Tissue and Cell Culture (abstr). Calgary, Canada: University of Calgary, p 163
Liu M-C, Hung Y-J, Shih S-C (1972) The in vitro production of plants from several tissues ofSaccharum species. J Agric Assoc China [New Series] 77:52–58
Maretzki A, Hiraki P (1980) Sucrose promotion of root formation in plantlets regenerated from callus ofSaccharum spp. Phyton 38:85–88
Maretzki A, Nickell LG (1973) Formation of protoplasts from sugarcane cell suspensions and the regeneration of cell cultures from protoplasts. Colloques internationaux CNRS, no. 212: Protoplastes et fusion de cellules somatiques végétales, pp 51–63
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nadar HM, Heinz DJ (1977) Root and shoot development from sugarcane callus tissue. Crop Sci 17:814–816
Nadar HM, Soepraptopo S, Heinz DJ, Ladd SL (1978) Fine structure of sugarcane (Saccharum sp.) callus and the role of auxin in embryogenesis. Crop Sci 18:210–216
Nagata T, Takebe I (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12–20
Nickell LG (1964) Tissue and cell cultures of sugarcane — another research tool. Hawaii Plant Rec 57:223–229
Nickell LG, Maretzki A (1969) Growth of suspension cultures of sugarcane cells in chemically defined media. Physiol Plant 22:117–125
Stewart JM, Hsu CL (1977) In ovulo embryo culture and seedling development of cotton (Gossypium hirsutum L.). Planta 137:113–117
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Larkin, P.J. Sugarcane tissue and protoplast culture. Plant Cell Tiss Organ Cult 1, 149–164 (1981). https://doi.org/10.1007/BF02318913
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02318913