Summary
A micromodification of the enzyme-linked immunosorbent assay (ELISA) was evaluated for its potential application in the immunodiagnosis of bovine trypanosomiasis. Serum samples from infected and non-infected Zebu cattle and samples from Friesian cattle with experimental infections were examined for the presence of trypanosomal antibodies. There were significant differences between the microELISA values obtained with samples from infected and non-infected cattle. During the course of infection microELISA values were found to fluctuate and the antibody response varied in individual animals. The test did not distinguish between infections withTrypanosoma brucei, T. vivax andT. congolense. There were no cross-reactions between trypanosome antigens and serum samples from cattle infected withT. theileri, Theileria parva, Th. mutans, Th. annulata, Babesia divergens andAnaplasma marginale.
Similar content being viewed by others
References
Ashkar, T. &Ochilo, M. (1972). The application of the indirect fluorescent antibody test to samples of sera and dried blood from cattle in the Lambwe Valley, South Nyanza, Kenya.Bulletin of the World Health Organization,47, 769–772.
Avrameas, S. &Ternynck, T. (1969). The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents.Immunochemistry,6, 53–66.
Binz, G. &Allsopp, R. (1972). Preliminary observations on the use of the latex agglutination test for detecting circulating trypanosomal antibodies in game animals in the Lambwe Valley.Bulletin of the World Health Organization,47, 781–783.
Clarkson, M. J., Cottrell, B. A. &Enayat, M. S. (1971). The indirect haemagglutination test in the study ofTrypanosoma vivax infections of sheep.Annals of Tropical Medicine and Parasitology,65, 335–340.
Engvall, E. &Perlmann, P. (1972). Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes.Journal of Immunology,109, 129–135.
Killick-Kendrick, R. (1968). The diagnosis of trypanosomiasis of livestock; a review of current techniques.The Veterinary Bulletin,38, 191–197.
Lanham, S. M. &Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose.Experimental Parasitology,28, 521–534.
Latif, B. M. A. &Adam, K. M. G. (1973). Differentiation ofTrypanosoma brucei, T. rhodesiense andT. gambiense by the indirect fluorescent antibody test.Bulletin of the World Health Organization,48, 401–407.
Luckins, A. G. (1972). Studies on bovine trypanosomiasis. Serum immunoglobulin levels in Zebu cattle exposed to natural infection in East Africa.British Veterinary Journal,128, 523–528.
Mehlitz, D. (1975). Serologische Untersuchungen zur Subgenus—Differenzierung und zur Antikorperpersistenz nach Trypanosomeninfektionen.Tropenmedizin und Parasitologie,26, 265–275.
Politzar, H. (1974). Serological studies on cattle experimentally infected with several species of African trypanosomes.Tropenmedizin und Parasitologie,25, 22–27.
Robson, J. (1972). The results of field trials of the capillary agglutination test for the detection of bovine trypanosomal antibodies, carried out in the Lambwe Valley.Bulletin of the World Health Organization,47, 779–780.
Robson, J. &Ashkar, T. S. (1972). Trypanosomiasis in domestic livestock in the Lambwe Valley area and a field evaluation of various diagnostic techniques.Bulletin of the World Health Organization,47, 727–734.
Schindler, R. (1972). Untersuchungen über die Brauchbarkeit serologischer Verfahren für die Diagnose der Rindertrypanosomiasis in Afrika.Zeitschrift für Tropenmedizin und Parasitologie,23, 78–88.
Soltys, M. A. &Woo, P. (1970). Biological differences of two sub-strains ofTrypanosoma brucei maintained by syringe passage in two different hosts.Annals of Tropical Medicine and Parasitology,64, 249–254.
Voller, A., Bidwell, D. &Bartlett, A. (1975a). A serological study on humanTrypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay.Tropenmedizin und Parasitologie,26, 247–251.
Voller, A., Draper, C., Bidwell, D. E. &Bartlett, A. (1975b). Microplate enzyme-linked immunosorbent assay for Chagas' disease.Lancet,1, 426–428.
Warburg, O. &Christian, W. (1941). Isolierung und Kristallisation des Garungsferments Enolase.Biochemische Zeitschrift,310, 384–421.
Wilson, A. J. (1969). Value of the indirect fluorescent antibody test as a serological aid to diagnosis ofGlossina-transmitted bovine trypanosomiasis.Tropical Animal Health and Production,1, 89–95.
Wilson, A. J. &Cunningham, M. P. (1971). Immunological aspects of bovine trypanosomiasis. IV. Patterns in the production of common antibodies.Tropical Animal Health and Production,3, 133–139.
Woo, P. T. K. &Soltys, M. A. (1972). Indirect haemagglutination test with sera of animals experimentally infected withTrypanosoma brucei andT. rhodesiense.Zeitschrift für Tropenmedizin und Parasitologie,23, 166–172.
Zwart, D., Perie, N. M., Keppler, A. &Goedbloed, E. (1973). A comparison of methods for the diagnosis of trypanosomiasis in East African domestic ruminants.Tropical Animal Health and Production,5, 79–86.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luckins, A.G. Detection of antibodies in Trypanosome-infected cattle by means of a microplate enzyme-linked immunosorbent assay. Trop Anim Health Prod 9, 53–62 (1977). https://doi.org/10.1007/BF02297393
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02297393