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HPLC characterization of phenolics in lignocellulosic materials

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Summary

The application of HPLC with an electrochemical detector for the determination of phenolics in lignocellulosic materials is reported. The separation of phenolic acids and aldehydes (gallic acid, p-hydroxybenzoic acid, vanillic acid, p-coumaric acid, syringic acid, ferulic acid, vanillin, syringaldehyde and p-hydroxybenzaldehyde) on two different columns (reversed phase C6 and styrene-divinylbenzene PLRP-S) is shown. Chromatograms of phenolic compounds in neutral, basic and oxidative extracts of wheat straw treated with NaOH and white rot fungusStropharia rugosoannulata are reported along with quantitative results.

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Authors and Affiliations

  1. Centro di Studio per la Conservazione dei Foraggi, Consiglio Nazionale delle Ricerche, Istituto di Agronomia Generale e Coltivazioni Erbacee, Università di Bologna, via Filippo Re, 8, 40126, Bologna, Italy

    G. C. Galletti & Roberta Piccaglia

  2. Dipartimento Chimico “G. Ciamician”, Università di Bologna, via Selmi 2, 40126, Bologna, Italy

    G. Chiavari & V. Concialini

  3. United States Department of Agriculture, Agricultural Research Service, 20705, Beltsville, MD, USA

    J. G. Buta

Authors
  1. G. C. Galletti
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  2. Roberta Piccaglia
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  3. G. Chiavari
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  4. V. Concialini
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  5. J. G. Buta
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Galletti, G.C., Piccaglia, R., Chiavari, G. et al. HPLC characterization of phenolics in lignocellulosic materials. Chromatographia 26, 191–196 (1988). https://doi.org/10.1007/BF02268150

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  • DOI: https://doi.org/10.1007/BF02268150

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