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Determination of Phenolic Compounds in Hypericum perforatum L. Aqueous Extracts by Gas Chromatography–Mass Spectrometry Using Solid-Phase Analytical Derivatization

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Abstract

We studied the gas chromatographic determination and identification of phenolic compounds in aqueous extracts of St. John’s wort (Hypericum perforatum L.) using solid-phase analytical derivatization in the sample preparation for analysis and mass spectrometric detection. A comparative analysis of the results of the determination of phenolic compounds using conventional analytical derivatization in solution and solid-phase analytical derivatization in aqueous extracts of St. John’s wort showed that the preparation of derivatives on an adsorbent shortens sample preparation, minimizes the volume of the extract, and expands the list of components to be determined. We found the optimal conditions for the adsorption of phenolic substances from aqueous extracts on the Strata C18-E adsorbent and solid-phase analytical derivatization with N,O-bis(trimethylsilyl)trifluoroacetamide. Gas chromatography–mass spectrometric characteristics were determined to identify compounds in the St. John’s wort aqueous extract. The results of the identification of compounds satisfactory agree with the HPLC–DAD data obtained using standard substances, allowing a conclusion that the solid-phase analytical derivatization of phenolic compounds is comparable in analytical capabilities to derivatization in solution.

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ACKNOWLEDGMENTS

The work was performed using scientific equipment from the Center for Collective Use “Ecological Analytical Center” of the Kuban State University.

Funding

The work was supported by the Russian Foundation for Basic Research, project no. 20-33-90045.

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Authors and Affiliations

  1. Department of Chemistry and High Technology, Kuban State University, 350040, Krasnodar, Russia

    Z. A. Temerdashev, E. A. Vinitskaya & V. V. Korobkova

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  1. Z. A. Temerdashev
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  2. E. A. Vinitskaya
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  3. V. V. Korobkova
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Corresponding author

Correspondence to Z. A. Temerdashev.

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The authors declare that they have no conflicts of interest.

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Translated by O. Zhukova

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Temerdashev, Z.A., Vinitskaya, E.A. & Korobkova, V.V. Determination of Phenolic Compounds in Hypericum perforatum L. Aqueous Extracts by Gas Chromatography–Mass Spectrometry Using Solid-Phase Analytical Derivatization. J Anal Chem 77, 1463–1473 (2022). https://doi.org/10.1134/S1061934822110120

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  • DOI: https://doi.org/10.1134/S1061934822110120

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