Summary
The aminoacid glutamine in aqueous solution and in conditions of high temperature and long term storage is partly transformed into pyroglutamic acid which exhibits potential neurotoxic effects.
Commercially available aminoacid mixtures supplemented with glutamine are heat-sterilized and some losses of glutamine and formation of pyroglutamic acid may occur.
The aim of the work was to set up an easy and reliable HPLC method which allows the determination of pyroglutamic acid as a degradation product of glutamine. The column was a 5 μm Hypersil ODS (100×4.6 mm) and the mobile phase 100% 0.007 M phosphate buffer pH 3.5.
Stability studies in different conditions of temperature and time of storage were performed on aminoacid mixture available in the commerce.
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Gandini, C., De Lorenzi, D., Kitsos, M. et al. HPLC determination of pyroglutamic acid as a degradation product in parenteral amino acid formulations. Chromatographia 36, 75–78 (1993). https://doi.org/10.1007/BF02263841
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DOI: https://doi.org/10.1007/BF02263841