Summary
A liquid-solid chromatographic pre-fractionation of naturally occurring phthalides has been developed. The LSC was carried out on a column of silica applying a 1–50% gradient elution of diethyl ether (containing 2% methanol) in pentane. The enrichment in the fractions led to better possibilities for a gas chromatographic separation and isolation allowing further studies by spectroscopic methods. Ligustilide may easily isomerize during GC as a result of ageing of the column.
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References
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M. J. M. Gijbels, J. J. C. Scheffer andA. Baerheim Svendsen, to be published.
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Gijbels, M.J.M., Scheffer, J.J.C. & Svendsen, A.B. Analysis of phthalides from umbelliferae by combined liquid-solid and gas-liquid chromatography. Chromatographia 14, 452–454 (1981). https://doi.org/10.1007/BF02263532
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DOI: https://doi.org/10.1007/BF02263532