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Applications of minirhizotrons to understand root function in forests and other natural ecosystems

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Abstract

Minirhizotrons have proved useful to understand the dynamics and function of fine roots. However, they have been used comparatively infrequently in forests and other natural plant communities. Several factors have contributed to this situation, including anomalous root distributions along the minirhizotron surface and the difficulty of extracting data from minirhizotron images. Technical and methodological advances have ameliorated some of these difficulties, and minirhizotrons have considerable potential to address some questions of long standing interest. These questions include more fully understanding the role of roots in carbon and nutrient cycling, rates of root decomposition, responses to resource availability and the functional significance of interactions between plant roots and soil organisms. Maximizing the potential for minirhizotrons to help us better understand the functional importance of fine roots in natural plant communities depends upon using them to answer only those questions appropriate to both their inherent strengths and limitations.

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References

  • Aber J D, Melillo J M, Nadelhoffer K J, McClaugherty C A and Pastor J 1985 Fine root turnover in forest ecosystems in relation to quantity and form of nitrogen availability: a comparison of two methods. Oecologia 66, 317–321.

    Article  Google Scholar 

  • Aerts R, Bakker C and De Caluwe H 1992 Root turnover as determinant of the cycling of C, N, and P in a dry heathland ecosystem. Biogeochemistry 15, 175–190.

    Google Scholar 

  • Aerts R, Berendse F, Klerk N M and Bakker C 1989 Root production and root turnover in two dominant species of wet heathlands. Oecologia 81, 374–378.

    Google Scholar 

  • Alexander I J and Fairley R I 1983 Effects of N fertilization on populations of fine roots and mycorrhizae in spruce humus. Plant and Soil 71, 49–53.

    Article  Google Scholar 

  • Allen MF 1991 The Ecology of Mycorrhizae. Cambridge University Press, Cambridge, UK. 184 p.

    Google Scholar 

  • Ball-Coelho B, Sampaio E V S B, Tiessen H and Stewart J W B 1992 Root dynamics in plant and ratoon crops of sugar cane. Plant and Soil 142, 297–305.

    Google Scholar 

  • Bates G H 1937 A device for the observation of root growth in the soil. Nature 139, 966–967.

    Google Scholar 

  • Berntson G M, Farnsworth E J and Bazzaz F A 1995 Allocation, within and between organs, and the dynamics of root length changes in two birch species. Oecologia 101, 439–447.

    Article  Google Scholar 

  • Bland W L and Dugas W A 1988 Root length density from minirhizotron observations. Agron. J. 80, 271–275.

    Google Scholar 

  • Bohm W, Maduakor H and Taylor H M 1977 Comparison of five methods for characterizing soybean rooting density and development. Agron. J, 69, 415–419.

    Google Scholar 

  • Box J E 1993 Use of the minirhizotron-miniature video camera technique for measuring root dynamics. Geoderma 56, 133–141.

    Google Scholar 

  • Box J E and Ramseur E L 1993 Minirhizotron wheat root data: comparisons to soil core root data. Agron. J. 85, 1058–1060.

    Google Scholar 

  • Bragg P L, Govi G and Cannell R Q 1983 A comparison of methods, including angled and vertical minirhizotrons, for studying root growth and distribution in a spring oat crop. Plant and Soil 73, 435–440.

    Article  Google Scholar 

  • Brundrett M, Murase G and Kendrick B 1990 Comparative anatomy of roots and mycorrhizae of common Ontario trees. Can. J. Bot. 68, 551–578.

    Google Scholar 

  • Buckland S T, Campbell C D, Mackie-Dawson L A, Horgan G W and Duff E I 1993 A method for counting roots observed in minirhizotrons and their theoretical conversion to root length density. Plant and Soil 153, 1–9.

    Google Scholar 

  • Chapin F S 1980 The mineral nutrition of wild plants. Annu. Rev. Ecol. Sys. 11, 233–260.

    Google Scholar 

  • Cheng W, Coleman D C and Box J E 1990 Root dynamics, production and distribution in agroecosystems on the Georgia piedmont using minirhizotrons. J. Appl. Ecol. 27, 592–604.

    Google Scholar 

  • Clobert J, Lebretom J D and Allaine D 1987 A general approach to survival rate estimation by recaptures or resightings of marked birds. Ardea 75, 133–142.

    Google Scholar 

  • Crick J C and Grime J P 1987 Morphological plasticity and mineral nutrient capture in two herbaceous species of contrasted ecology. New Phytol. 107, 403–414.

    Google Scholar 

  • Dubach M and Russelle M P 1995 Reducing the cost of estimating root turrnover with horizontally installed minirhizotrons. Agron. J. 87, 258–263.

    Google Scholar 

  • Eissenstat D M and Caldwell M M 1988 Seasonal timing of fine root growth in favorable microsites. Ecology 59, 870–873.

    Google Scholar 

  • Fahey 1992 Mycorrhizae and forest ecosystems. Mycorrhiza 1, 83–89.

    Article  Google Scholar 

  • Fahey T J and Arthur M A 1994 Further studies of root decomposition following harvest of a northern hardwoods forest. For. Sci. 40, 618–629.

    Google Scholar 

  • Fahey T J and Hughes J W 1994 Fine root dynamics in a northern hardwood forest ecosystem, Hubbard Brook Experimental Forest, NH. J. Ecol. 82, 533–548.

    Google Scholar 

  • Fahey T J, Hughes J W, Pu M and Arthur M A 1988 Root decomposition and nutrient flux following whole-tree harvest of northern hardwood forest. For. Sci. 34, 744–768.

    Google Scholar 

  • Fitter A H 1987 An architectural approach to the comparative ecology of plant root systems. New Phytol. 106, 61–77.

    Google Scholar 

  • Goldfarb D, Hendrick R and Pregitzer K 1990 Seasonal nitrogen and carbon concentrations of white, brown and woody fine roots of sugar maple (Acer saccharum Marsh). Plant and Soil 126, 144–148.

    Article  Google Scholar 

  • Gower S T, Vogt K A and Grier C C 1992 Carbon dynamics of Rocky mountain Douglas-fir: influence of water and nutrient availability. Ecol. Monogr. 62, 43–65.

    Google Scholar 

  • Gregory P J 1979 A periscope method for observing root growth and distribution in field soil. J. Exp. Bot. 30. 205–214.

    Google Scholar 

  • Grime J P, Campbell B D, Mackey J M L and Crick J C 1991 Root plasticity, nitrogen capture and competitive ability.In Plant Root Growth, an Ecological Perspective. Ed. D Atkinson. pp 381–397. Blackwell Scientific Publishers, London, UK.

    Google Scholar 

  • Gross K L, Peters A and Pregitzer K S 1993 Fine root growth and demographic responses to nutrient patches in four old-field plant species. Oecologia 95, 61–64.

    Google Scholar 

  • Hansson A, Ai-Fen Z and Andrén O 1994 Fine-root growth dynamics of two shrubs in semiarid rangeland in inner Mongolia, China. Ambio 23, 225–228.

    Google Scholar 

  • Hansson A, Aifen Z and Andrén O 1995 Fine-root production and mortality in degraded vegetation in Horqin sandy rangeland in inner Mongolia, China. Arid Soil Res. Rehab. 9, 1–13.

    Google Scholar 

  • Hansson A and Andrén O 1987 Root dynamics in barley, lucerne and meadow fescue investigated with a mini-rhizotron technique. Plant and Soil 103, 33–38.

    Google Scholar 

  • Haverkort A J, Groenwold J and Van de Waart M 1994 The influence of cyst nematodes and drought on potato growth. Eur. J. Plant Path. 100, 381–394.

    Article  Google Scholar 

  • Heeraman D A, Crown P H and Juma N G 1993 A color composite technique for detecting root dynamics of barley (Hordeum vulgare L.) from minirhizotron images. Plant and Soil 157, 275–287.

    Article  Google Scholar 

  • Heeraman D A and Juma N G 1993 A comparison of minirhizotron, core and monolith methods for quantifying barley (Hordeum vulgare L.) and fababean (Vicia faba L.) root distribution. Plant and Soil 148, 29–41.

    Article  Google Scholar 

  • Hendrick R L and Pregitzer K S 1992a The demography of fine roots in a northern hardwood forest. Ecology 73, 1094–1104.

    Google Scholar 

  • Hendrick R L and Pregitzer K S 1992b Spatial variation in tree root distribution and growth associated with minirhizotrons. Plant and Soil 143, 283–288.

    Google Scholar 

  • Hendrick R L and Pregitzer K S 1993a The dynamics of fine root length, biomass, and nitrogen content in two northern hardwood ecosystems. Can. J. For. Res. 23, 2507–2520.

    Google Scholar 

  • Hendrick R L and Pregitzer K S 1993b Patterns of fine root mortality in two sugar maple forests. Nature 361, 59–61.

    Article  Google Scholar 

  • Hooker J E and Atkinson D 1992 Application of computer-aided image analysis to studies of arbuscular endomycorrhizal fungi effects on plant root system morphology and dynamics. Agronomie 12, 821–824.

    Google Scholar 

  • Hooker J E, Black K E, Perry R L and Atkinson D 1995 Arbuscular mycorrhizal fungi induced alteration to root longevity of polar. Plant and Soil 172, 327–329.

    Article  Google Scholar 

  • Joslin J D and Henderson G S 1987 Organic matter and nutrients associated with fine root turnover in a white oak stand. For. Sci. 33, 330–346.

    Google Scholar 

  • Kalbfleisch J D and Prentice R L 1980 The Statistical Analysis of Failure Time Data. John Wiley and Sons, New York, USA. 321 p.

    Google Scholar 

  • Kätterer T, Fabiao A, Madeira M, Ribeiro C and Steen E 1995 Fine-root dynamics, soil moisture and soil carbon content in aEucalyptus globulus plantation under different irrigation and fertilisation regimes. For. Ecol. Manage. 74, 1–12.

    Article  Google Scholar 

  • Keng J C W 1988 Monitoring corn root development with a minirhizotron in confined growth boxes. Agron. J. 80, 287–291.

    Google Scholar 

  • Keyes M R and Grier C C 1981 Above-and belowground net primary production in 40-year-old Douglas-fir stands on low and high productivity sites. Can. J. For. Res. 11, 599–605.

    Google Scholar 

  • Kochenderfer J N 1973 Root distribution under some forest types native to West Virginia. Ecology 54, 445–448.

    Google Scholar 

  • Kosola K R, Eissenstat D M and Graham J C 1995 Root demography of mature citrus trees: the influence ofPhytophthora nicotianae. Plant and Soil 171, 283–288.

    Google Scholar 

  • Kurz W A and Kimmins J P 1987 Analysis of error in methods used to determine fine root production in forest ecosystems: a simulation approach. Can. J. For. Res. 17, 909–912.

    Google Scholar 

  • Levan M A, Ycas J W and Hummel J W 1987 Light leak effects on near-surface soybean rooting observed with minirhizotrons.In Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics. Ed. H M Taylor. American Society of Agronomy Special Publication Number 50. pp 89–98. Am. Soc. Agron., Madison, WI, USA.

    Google Scholar 

  • Lussenhop J and Fogel R 1993 Observing soil biota in situ. Geoderma 56, 25–36.

    Google Scholar 

  • Lussenhop, Fogel R and Pregitzer K 1991 A new dawn for soil biology: video analysis of root-soil-microbial-faunal interactions. Agric. Ecosyst. Environ. 34, 235–249.

    Article  Google Scholar 

  • Majdi H, Smucker A J M and Persson H 1992 A comparison between minirhizotron and monolith sampling methods for measuring root growth of maize (Zea mays L.). Plant and Soil 147, 127–134.

    Article  Google Scholar 

  • McClaugherty C A, Aber J D and Melillo J M 1984 Decomposition dynamics of fine roots in forested ecosystems. Oikos 42, 378–386.

    Google Scholar 

  • McKay H and Coutts M P 1989 Limitations placed on forestry production by the root system. Aspects Appl. Biol. 22, 245–254.

    Google Scholar 

  • Merrill S D, Doering E J and Reichman G A 1987 Application of a minirhizotron with flexible, pressurized walls to a study of corn root growth.In Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics. Ed. H M Taylor. American Society of Agronomy Special Publication Number 50. pp 131–143. Am Soc. Agron., Madison, WI, USA.

    Google Scholar 

  • Merrill S D and Upchurch D R 1994 Converting root numbers observed at minirhizotrons to equivalent root length density. Soil Sci. Soc. Am. J. 58, 1061–1067.

    Google Scholar 

  • Meyer W S and Barrs H D 1985 Non-destructive measurement of wheat roots in large undisturbed and repacked clay soil cores. Plant and Soil 85, 237–247.

    Google Scholar 

  • Murphy J A, Hendricks M G, Rieke P E, Smucker A J M and Branham B E 1994 Turfgrass root systems evaluated using the minirhizotron and video recording methods. Agron. J. 86, 247–250.

    Google Scholar 

  • Nadelhoffer K J, Aber J D and Melillo J M 1985 Fine roots, net primary production and nitrogen availability: a new hypothesis. Ecology 66, 1377–1390.

    Google Scholar 

  • Nater E A, Nater K D and Baker J M 1992 Application of artificial neural system algorithms to image analysis of roots in soil. I. Initial results. Geoderma 53, 237–253.

    Google Scholar 

  • Newman E I 1966 A method of estimating the total length of roots in a sample. J. Appl. Ecol. 3, 139–145.

    Google Scholar 

  • Pregitzer K S, Hendrick R L and Fogel R 1993 The demography of fine roots in response to patches of water and nitrogen. New Phytol. 125, 575–580.

    Google Scholar 

  • Pregitzer K S, Zak D R, Cuztis P S, Kubiske M E, Teeri J A and Vogel C S 1995. Atmospheric CO2, soil nitrogen and turnover of fine roots. New Phytol. 129, 579–585.

    Google Scholar 

  • Rush C M, Upchurch D R and Gerik T J 1984 In situ observations ofPhymatotrichum omnivorum with a boroscope mini-rhizotron system. Am. Phytopath. Soc. 74, 104–105.

    Google Scholar 

  • Samson B K and Sinclair T R 1994 Soil core and minirhizotron comparison for the determination of root length density. Plant and Soil 161, 225–232.

    Article  Google Scholar 

  • Sanders J L and Brown D A 1978 A new fiber optic technique for measuring root growth of soybeans under field conditions. Agron. J. 70, 1073–1077.

    Google Scholar 

  • Santantonio D and Grace J 1987 Estimating fine root production and turnover from biomass and decomposition data: a compartment flow model. Can. J. For. Res. 17, 900–908.

    Google Scholar 

  • Singh J S, Laurenroth W K, Hunt H W and Swift D M 1984 Bias and random errors in estimators of net root production: a simulation approach. Ecology 65, 1760–1764.

    Google Scholar 

  • Smit A L and Zuin A 1996 Root growth dynamics of Brussels sprouts (Brassica olearacea, var.gemmifera) and leeks (Allium porrum L.) as reflected by root length, root colour and UV fluorescence. Plant and Soil 185, 269–278.

    Google Scholar 

  • Smucker A J M 1990 Quantification of root dynamics in agroecological systems. Remote Sens. Rev. 5, 237–248.

    Google Scholar 

  • Smucker A J M, Ferguson J C, DeBruyn W P, Belford R K and Ritchie J T 1987 Image analysis of video recorded plant root systems.In Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics. Ed. H M Taylor. American Society of Agronomy Special Publication Number 50. pp 67–80. Am. Soc. Agron., Madison, WI, USA.

    Google Scholar 

  • Snider R J, Snider R and Smucker A J M 1990 Collembolan populations and root dynamics in Michigan agroecosystems.In Rhizosphere Dynamics. Eds. J E Box and L C Hammond. Westview Press, Boulder, CO, USA.

    Google Scholar 

  • Taylor H M 1987 Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics, American Society of Agronomy Special Publication Number 50. Am. Soc. Agron., Madison, WI, USA. 143 p.

    Google Scholar 

  • Tennant D 1975 A test of a modified line intersect method of estimating root length. J. Ecol. 3, 995–1001.

    Google Scholar 

  • Upchurch D R 1987 Conversion of minirhizotron root intersections to root length density.In Minirhizotron Observation Tubes: Methods and Applications for Measuring Rhizosphere Dynamics. Ed. H M Taylor. American Society of Agronomy Special Publication Number 50. pp 51–66. Am. Soc. Agron., Madison, WI, USA.

    Google Scholar 

  • Upchurch D R and Ritchie J T 1983 Root observations using a video recording system in mini-rhizotrons. Agron. J. 75, 1009–1015.

    Google Scholar 

  • Upchurch D R and Ritchie J T 1984 Battery-operated color video camera for root observations in minirhizotrons. Agron. J. 74, 1015–1017.

    Google Scholar 

  • Van Noordwijk M, de Jager A and Floris J 1985 A new dimension to observations in minirhizotrons: a stereoscopic view on root photographs. Plant and Soil 86, 447–453.

    Google Scholar 

  • Van Vuuren M M I, Berendse F and De Visser W 1993 Species and site differences in the decomposition of litters and roots from wet heathlands. Can J. Biot. 71, 167–173.

    Google Scholar 

  • Volkmar K M 1993 A comparison of minirhizotron techniques for estimating root length density in soils of different bulk densities. Plant and Soil 157, 239–245.

    Article  Google Scholar 

  • Vos J and Groenwold J 1983 Estimation of root densities by observation tubes and endoscope. Plant and Soil 74, 295–300.

    Google Scholar 

  • Wang Z, Burch W H, Mou P, Jones R H and Mitchell R J 1995 Accuracy of visible and ultraviolet light for estimating live root proportions with minirhizotrons. Ecology 76, 2330–2334.

    Google Scholar 

  • Whitford W G, Stinnett K and Anderson J 1988 Decomposition of roots in a Chihuahuan Desert ecosystem. Oecologia 75, 8–11

    Google Scholar 

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  1. D.B. Warnell School of Forest Resources, University of Georgia, 30602, Athens, GA, USA

    Ronald L. Hendrick & Kurt S. Pregitzer

  2. School of Forestry and Wood Products, Michigan Technological University, 49931, Houghton, MI, USA

    Ronald L. Hendrick & Kurt S. Pregitzer

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  1. Ronald L. Hendrick
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  2. Kurt S. Pregitzer
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Hendrick, R.L., Pregitzer, K.S. Applications of minirhizotrons to understand root function in forests and other natural ecosystems. Plant Soil 185, 293–304 (1996). https://doi.org/10.1007/BF02257535

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