Skip to main content
SpringerLink
Log in

Apical localization of glycoproteins in gastric mucous cells

  • Published:
The American Journal of Digestive Diseases Aims and scope Submit manuscript

Summary

The staining of mucous cells in the gastric fundic mucosa has been studied in various species, including man. In most cases, a mucous coat is observed, at the apical pole of mucous cells, in the epithelium and in the deep part of the gastric pits. This coat stands between the cell cytoplasm and the gastric lumen. The surface coat stains positively with PAS and alcian blue, and its characteristics differ depending on the species and the topography of the cell. In some cases, particularly in the cat, the coat is a very thin line, coalescent with the plasma membrane; this could be the optical appearance of the polysaccharidic extraneous coat described in ultrastructure. More often the coat is thick, projecting into either the gastric lumen or the cell cytoplasm, its significance being questionable. In the deep part of the gastric pits of the rat, histoautoradiography after injection of radiosulfate demonstrates an incorporation of sulfate-35 in the apical pole of these cells, encircling the plasma membrane. This mucous apical layer is thus rich in sulfated glycoproteins. This coating of the apical pole in the cells regenerating the mucosa could intervene in the resistance of the mucosa against pepsin. Indeed, such molecules can inhibit peptic proteolysis by a specific mechanism—the formation of a complex when joined with the proteins of the cell surface.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bernard, C.,Leçons de Physiologie Expérimentale Appliquée à la Médecine (Vol. 2). Bailliere, Paris, 1856, p. 406.

    Google Scholar 

  2. Komarov, S. A. Isolation of mucoitin sulfuric acid from canine gastric juice.J Biol Chem 109:177, 1935.

    Google Scholar 

  3. Martin, F., andLambert, R. “Rôle des Glycoprotéines Sulfatées dans les Mécanismes de Protection de la Muqueuse Gastrique.” InXVI o Coll. on Protéides on the Biological Fluids Bruges, May 1968. Pergamon, New York. In press.

  4. Curran, R. C. The histochemistry of mucopolysaccharides.Int Rev Cytol 17:149, 1964.

    PubMed  Google Scholar 

  5. Gerard, A., Lev, R., andGlass, G. B. J. Histochemical study of the mucosubstances in the canine stomach. I. The resting mucosa.Amer J Dig Dis 12:891, 1967.

    PubMed  Google Scholar 

  6. Spicer, S. S. A correlative study of the histochemical properties of rodent acid mucopolysaccharides.J. Histochem Cyiochem 8:18, 1960.

    Google Scholar 

  7. Thompson, S. M. Selected Histochemical and Histopathological Methods. Thomas, Springfield, Ill., 1966.

    Google Scholar 

  8. Lev, R., andSpicer, S. S. Specific staining of sulphate groups with alcian blue at low pH.J Histochem Cytochem 12:309, 1964.

    PubMed  Google Scholar 

  9. Ito, S. “Anatomic Structure of the Gastric Mucosa.” InHandbook of Physiology (Vol. 2,Secretion). American Physiological Society, Washington, D. C., 1967, pp. 705–741.

    Google Scholar 

  10. Ito, S., andWinchester, R. J. The fine structure of the gastric mucosa in the rat.J Cell Biol 16:541, 1963.

    Article  PubMed  Google Scholar 

  11. Gerard, A. Etudes histochimiques et histophysiologiques des glycoproteines de la muqueuse gastrique chez le chien.Arch Biol (Liège) 79:1, 1968.

    Google Scholar 

  12. Bennett, H. S. Morphological aspects of extracellular polysaccharids.J Histochem Cytochem 11:14, 1963.

    Google Scholar 

  13. Brandt, P. W. A consideration of the extraneous coats of the plasma membrane.Circulation 26:1075, 1962.

    PubMed  Google Scholar 

  14. Fawcett, D. W. Physiologically significant specializations of the cell surface.Circulation 26:1105, 1962.

    PubMed  Google Scholar 

  15. Fawcett, D. W. Surface specializations of absorbing cells.J Histochem Cytochem 13:75, 1965.

    PubMed  Google Scholar 

  16. Ito, S. The enteric surface coat on cat intestinal microvilli.J Cell Biol 27:475, 1965.

    Article  PubMed  Google Scholar 

  17. Ito, S. Radioactive labelling of the surface coat on enteric microvilli.Anat Rec 151:489, 1965.

    Google Scholar 

  18. Revel, J. P. A stain for the ultrastructural localization of acid mucopolysaccharides.J Microscopie 3:535, 1965.

    Google Scholar 

  19. Ito, S., andRevel, J. P. Incorporation of radioactive sulfate and glucose on the surface coat of enteric microvilli.J Cell Biol 23:44A, 1964.

  20. Hakkinen, I., Hartiala, K., andTerho, T. The fractionation and characterisation of the acid polysaccharides of the gastric wall of the dog.Acta Chem Scand 19:800, 1965.

    PubMed  Google Scholar 

  21. Martin, F., Berard, A., Jouvenceaux, A., andLambert, R. Isolement d'un mucopolysaccharide sulfaté à partir du suc gastrique humain.C R Soc Biol (Paris)161:848, 1967.

    Google Scholar 

  22. Martin, F., Lambert, R., Martin, M. M., Vuez, J. L., andBerard, A. Les glycoprotéines sulfatées dans la sécrétion gastrique humaine.Biol Gastroent (Paris)1:5, 1968.

    Google Scholar 

  23. Hakkinen, I., andHartiala, K. The effect of cinchophen on the acid polysaccharides of the gastric and duodenal wall in dog.Acta Physiol Scand 66:326, 1966.

    PubMed  Google Scholar 

  24. Berard, A., Martin, F., andLambert, R. Etude par diffusion sur gelose de l'inhibition de la protéolyse peptique par les polysaccharides sulfatés.Gastroenterologia 108:209, 1967.

    PubMed  Google Scholar 

  25. Denko, C. W. The effect of phenylbutazone and its derivatives oxyphen butazone and sulfin pyrazone on S35 sulfate incorporation in cartilage and stomach.J Lab Clin Med 63:953, 1964.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. From the Unité de Recherches de Physiopathologie Digestive, Hôpital Edouard Herriot, and Clinique Médicale, Lyon, France

    R. Lambert M.D., F. Martin M.D., C. Andre M.D., L. Descos M.D. & G. Vouillon M.D.

Authors
  1. R. Lambert M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Martin M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Andre M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Descos M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Vouillon M.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The electron microscopy studies were performed by Prof. C. Girod. Laboratoire d'Histologie, Faculté de Médecine de Lyon.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lambert, R., Martin, F., Andre, C. et al. Apical localization of glycoproteins in gastric mucous cells. Digest Dis Sci 13, 941–951 (1968). https://doi.org/10.1007/BF02232916

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02232916

Keywords

Search

Navigation