Abstract
The pronotal and elytral defensive secretions of 10Oreina species were analyzed. Species feeding on Apiaceae, i.e.,O. frigida andO. viridis, or on Cardueae (Asteraceae), i.e.,O. bidentata, O. coerulea, andO. virgulata, produce species-specific complex mixtures of autogenous cardenolides.O. melanocephala, which feeds onDoronicum clusii (Senecioneae, Asteraceae), devoid of pyrrolizidine alkaloids (PAs) in its leaves, secretes, at best, traces of cardenolides. Sequestration of host-plant PAs was observed in all the other species when feeding on Senecioneae containing these alkaloids in their leaves.O. cacaliae is the only species that secretes host-derived PA N-oxides and no autogenous cardenolides. Differences were observed in the secretions of specimens collected in various localities, because of local differences in the vegetation. The other species, such asO. elongata, O. intricata, andO. speciosissima, have a mixed defensive strategy and are able both to synthesize de novo cardenolides and to sequester plant PA N-oxides. This allows a great flexibility in defense, especially inO. elongata andO. speciosissima, which feed on both PA and non-PA plants. Populations of these species were found exclusively producing cardenolides, or exclusively sequestering PA N-oxides, or still doing both, depending on the local availability of food-plants. Differences were observed between species in their ability to sequester different plant PA N-oxides and to transform them. Therefore sympatric species demonstrate differences in the composition of their host-derived secretions, also resulting from differences in host-plant preference. Finally, within-population individual differences were observed because of local plant heterogeneity in PAs. To some extent these intrapopulation variations in chemical defense are tempered by mixing diet and by the long-term storage of PA N-oxides in the insect body that are used to refill the defensive glands.
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References
Bourdonneé, J.-C., andDoguet, S. 1991. Données sur la biosystématique des Chrysolinina L.S. (Coleoptera: Chrysomelidae: Chrysomelinae).Ann. Soc. Entomol. Fr. 27:24–69.
Bowers, M.D. 1992. The evolution of unpalatability and the cost of chemical defense in insects, pp. 216–244,in B.D. Roitberg and M.B. Isman (eds.). Insect Chemical Ecology. An Evolutionary Approach. Chapman & Hall, New York.
Brower, L.P. 1970. Plant poisons in a terrestrial food chain with implications for mimicry theory.Proc. Annu. Biol. Colloq. Oregon State Univ. 29:69–82.
Dobler, S., andRowell-Rahier, M. 1994a. Production of cardenolides versus sequestration of pyrrolizidine alkaloids in larvae ofOreina species (Coleoptera, Chrysomelidae).J. Chem. Ecol. 20:555–568.
Dobler, S., andRowell-Rahier, M. 1994b. Response of a leaf beetle to two food plants, only one of which provides a sequestrable defensive chemical.Oecologia 97:271–277.
Eggenberger, F., andRowell-Rahier, M. 1991. Chemical defense and genetic variation. Interpopulational study ofOreina gloriosa (Coleoptera: Chrysomelidae).Naturwissenschaften 78:317–320.
Eggenberger, F., andRowell-Rahier, M. 1992. Genetic component of variation in chemical defense ofOreina gloriosa (Chrysomelidae: Coleoptera).J. Chem. Ecol. 18:1375–1387.
Eggenberger, F., andRowell-Rahier, M. 1993. Physiological sources of variation in chemical defense ofOreina gloriosa (Coleoptera: Chrysomelidae).J. Chem. Ecol. 19:395–410.
Eggenberger, F., Daloze, D., Pasteels, J.M., andRowell-Rahier, M. 1992. Identification and seasonal quantification of defensive secretion components ofOreina gloriosa (Coleoptera: Chrysomelidae).Experientia 48:1173–1179.
Ehmke, A., Rowell-Rahier, M., Pasteels, J.M., andHartmann, T. 1991. Sequestration of ingested [14C]senecionine N-oxide in the exocrine defensive secretions of chrysomelid beetles.J. Chem. Ecol. 17:2367–2379.
Freude, H., Harde, K.W., andLohse, G.A. 1966. Die Käfer Mitteleuropas, Vol. 9. Goecke & Evers Verlag, Krefeld, Germany.
Hartmann, T., andWitte, L. 1995. Chemistry, biology and chemoecology of the pyrrolizidine alkaloids, pp. 155–233,in S.W. Pelletier (ed.). Alkaloids: Chemical and Biological Perspectives, Vol. 9, Pergamon Press, Oxford.
Kühnelt, W. 1984. Monographie der BlattkäfergattungChrysochloa (Coleoptera, Chrysomelidae), pp. 171–287,in Sitzung der mathem.-naturw. Klasse am 12 Oktober 1984, Springer-Verlag, Vienna.
Pasteels, J.M., Braekman, J.-C., andDaloze, D. 1988a. Chemical defense in the Chrysomelidae, pp. 233–252,in P. Jolivet, E. Petitpierre, and T. H. Hsiao (eds.). Biology of the Chrysomelidae. Kluwer, Dordrecht, Netherlands.
Pasteels, J.M., Rowell-Rahier, M., Randoux, T., Braekman, J.-C., andDaloze, D. 1988b. Pyrrolizidine alkaloids of probable host-plant origin in the pronotal and elytral secretion of the leaf beetleOreina cacaliae.Entomol. Exp. Appl. 49:55–58.
Pasteels, J.M., Ehmke, A., Eggenberger, F., Rowell-Rahier, M., andHartmann, T. 1992. Chemical defense in chrysomelid leaf beetles: storage of host-derived pyrrolizidine alkaloids versus de novo synthesized cardenolides.Naturwissenschaften 79:521–523.
Pasteels, J.M., Rowell-Rahier, M., Braekman, J.-C., andDaloze, D. 1994. Chemical defense in chrysomelid leaf beetles updated, pp. 289–301,in P.H. Jolivet, M.L. Cox and E. Petipierre (eds.). Novel Aspects of the Biology of Chrysomelidae. Kluwer, Dordrecht, Netherlands.
Rowell-Rahier, M. 1992. Genetic structure of leaf-beetle populations: Microgeographic and sexual differentiation inOreina cacaliae andO. speciosissima.Entomol. Exp. Appl. 65:247–257.
Rowell-Rahier, M., andPasteels, J.M. 1992. Third trophic level influences of plant allelochemicals, pp. 243–277,in G.A. Rosenthal and M.R. Berenbaum (eds.). Herbivores. Their Interactions with Secondary Plant Metabolites, 2nd ed., Vol. II. Ecological and Evolutionary Processes. Academic Press, San Diego.
Rowell-Rahier, M., andPasteels, J.M. 1994. A comparison between allozyme data and phenotypic distances from defensive secretion inOreina leaf-beetles (Chrysomelinae).J. Evol. Biol. 7:489–500.
Rowell-Rahier, M., Witte, L., Ehmke, A., Hartmann, T., andPasteels, J.M. 1991. Sequestration of plant pyrrolizidine alkaloids by chrysomelid beetles and selective transfer into the defensive secretions.Chemoecology 2:41–48.
Rowell-Rahier, M., Pasteels, J.M., Alonso-Mejia, A., andBrower, L.P. 1995. Relative unpalatability of leaf-beetles with either biosynthesized or sequestered chemical defense.Anim. Behav. 49:709–714.
Van Oycke, S., Randoux, T., Braekman, J.-C., Daloze, D., andPasteels, J.M. 1988. New cardenolide glycosides from the defense glands of Chrysosilina beetles (Coleoptera: Chrysomelidae).Bull. Soc. Chim. Belg. 97:297–311.
Witte;, L., Rubiolo, P., Bicchi, C., andHartmann, T. 1993. Comparative analysis of pyrrolizidine alkaloids from natural sources by gas chromatography-mass spectrometry.Phytochemistry 32:187–196.
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Pasteels, J.M., Dobler, S., Rowell-Rahier, M. et al. Distribution of autogenous and host-derived chemical defenses inOreina leaf beetles (Coleoptera: Chrysomelidae). J Chem Ecol 21, 1163–1179 (1995). https://doi.org/10.1007/BF02228318
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DOI: https://doi.org/10.1007/BF02228318