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Effect of exercise on tissue anti-oxidant capacity and heart electrical properties in male and female rats

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Abstract

We studied the changes in the anti-oxidant capacity of tissues, such as heart, liver, and blood in male and female rats, as a parameter for evaluating oxidative stress after either a prolonged (210 min) or an exhausting bout of swimming. Furthermore, we also investigated exercise-induced changes in the electrophysiological properties, measured in vitro, of papillary muscle fibres. Small decreases of anti-oxidant capacities after prolonged exercise [0.10 (SEM 0.04) in heart, 0.43 (SEM 0.19) in liver, 0.22 (SEM 0.05) in blood] and greater decreases after exhausting exercise [0.23 (SEM 0.04) in heart, 0.90 (SEM 0.29) in liver, 0.34 (SEM 0.04) in blood] were found in tissues from the male rats. For the female rats, similar changes were found only in the blood [0.11 (SEM 0.07) and 0.35 (SEM 0.06) for prolonged and exhausting exercise, respectively]. Liver and heart anti-oxidant capacity remained unchanged after prolonged exercise, while after exhausting swimming it underwent a decrease almost the same as found in the male rats, though the swimming time to exhaustion (endurance capacity) was much greater [706 (SEM 10) min and 444 (SEM 32) min for the females and males, respectively]. The duration of the action potential, recorded from papillary muscle fibres, underwent changes related to the decreases in heart anti-oxidant capacity. In fact, the action potential duration (APD) was shorter only in preparations from the male rats after prolonged exercise, but in all preparations after exhausting exercise. After such exercise, the APD was similar for the male and female rats [37.1 (SEM 3.4) ms and 37.0 (SEM 3.6) ms, respectively]. Such a pattern was independent of stimulation frequency, since it was found substantially unchanged when the frequency was increased from 1 to 5 Hz. We concluded that the different susceptibilities to the effects of physical exercise, exhibited by tissues from these male and female rats might have been related to different capacities to oppose oxidative stress effectively.

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Authors and Affiliations

  1. Dipartimento di Fisiologia Generale ed Ambientale dell'Università di Napoli, Via Mezzocannone 8, I-80134, Naples, Italy

    P. Venditti, M. C. Piro & S. Di Meo

  2. Cattedra di Attività Motoria-Istituto Superiore di Educazione Fisica di Napoli, Naples, Italy

    G. Artiaco

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  1. P. Venditti
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  2. M. C. Piro
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  3. G. Artiaco
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  4. S. Di Meo
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Venditti, P., Piro, M.C., Artiaco, G. et al. Effect of exercise on tissue anti-oxidant capacity and heart electrical properties in male and female rats. Europ. J. Appl. Physiol. 74, 322–329 (1996). https://doi.org/10.1007/BF02226928

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