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Functional characterisation of a purified homogeneous Photosystem II core complex with high oxygen evolution capacity from spinach

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Abstract

The functional properties of a purified homogeneous spinach PS II-core complex with high oxygen evolution capacity (Haag et al. 1990a) were investigated in detail by measuring thermoluminescence and oscillation patterns of flash induced oxygen evolution and fluorescence quantum yield changes. The following results were obtained:

  1. a)

    Depending on the illumination conditions the PS II-core complexes exhibit several thermoluminescence bands corresponding to the A band, Q band and Zv band in PS II membrane fragments. The lifetime of the Q band (Tmax=10°C) was determined to be 8s at T=10°C. No B band corresponding to S2QB − or S3QB − recombination could be detected.

  2. b)

    The flash induced transient fluorescence quantum yield changes exhibit a multiphasi relaxation kinetics shich reflect the reoxidation of Q −A . In control samples without exogenous acceptors this process is markedly slower than in PS II membrane fragments. The reaction becomes significantly retarded by addition of 10 μM DCMU. After dark incubation in the presence of K3[Fe(CN)6

  3. c)

    Excitation of dark-adapted samples with a train of short saturating flashes gives rise to a typical pattern dominated by a high O2 yield due to the third flash and a highly damped period four oscillation. The decay of redox states S2 and S3 are dominated by short life times of 4.3 s and 1.5 s, respectively, at 20°C.

The results of the present study reveal that in purified homogeneous PS II-core complexes with high oxygen evolution isolated from higher plants by β-dodecylmaltoside solubilization the thermodynamic properties and the kinetic parameters of the redox groups leading to electron transfer from water to QA are well preserved. The most obvious phenomenon is a severe modification of the QB binding site. The implications of this finding are discussed.

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Abbreviations

β-DM:

β-dodecyl-maltoside

PS II:

Photosystem II

RC:

reaction center

QA, QB :

Primary and secondary quinone acceptors

Ph-p-BQ:

phenylparabenzoquinone

Chl:

chlorophyll

TL:

thermoluminescence

LHC II:

Photosystem II light-harvesting antenna complex

MES:

4-morpholineethanesulfonic acid

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

DCMU:

3-(3,4-dichlorophenyl)-1-1-dimethylurea

DCBQ:

dichlorobenzoquinone

Si :

sequential oxidation states of the water oxidizing enzyme in the model of B. Kok (i=0...4)

YZ :

tyrosine 161 of D1 protein, which is the secondary donor of Photosystem II

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Authors and Affiliations

  1. Max-Volmer-Institute for Biophysical Chemistry, Technical University Berlin, 1000, Berlin 12, Germany

    H. M. Gleiter, E. Haag & G. Renger

  2. RIKEN, The Institute for Physical and Chemical Research, 351-01, Wako, Saitama, Japan

    H. M. Gleiter & Y. Inoue

Authors
  1. H. M. Gleiter
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  2. E. Haag
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  3. Y. Inoue
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  4. G. Renger
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Gleiter, H.M., Haag, E., Inoue, Y. et al. Functional characterisation of a purified homogeneous Photosystem II core complex with high oxygen evolution capacity from spinach. Photosynth Res 35, 41–53 (1993). https://doi.org/10.1007/BF02185410

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