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Reminiscence about‘Chloropseudomonas ethylicum’ and the FMO-protein

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Abstract

In 1961 the green sulfur bacterium-containing mixed culture known as‘Chloropseudomonas ethylicum’ was brought to Brookhaven National Laboratory (USA) from Moscow State University (USSR). The water-soluble bacteriochlorophylla-protein (FMO-protein) was extracted, purified and characterized by absorption and circular dichroism spectroscopy, by X-ray crystallography and by primary structure determination.

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Abbreviations

BChl:

bacteriochlorophyll

CD:

circular dichroism

References

  • Bobe FW, Pfennig N, Swanson KL and Smith KM (1990) Red shift of absorption maxima in Chlorobiineae through enzymic methylation of their antenna bacteriochlorophylls. Biochemistry 29: 4340–4348

    Google Scholar 

  • Broch-Due M and Ormerod JG (1978) Isolation of a BChlc mutant fromChlorobium with BChld by cultivation at low light intensity. FEMS Microbiol Lett 3: 305–308

    Google Scholar 

  • Daurat-Larroque ST, Brew K and Fenna RE (1986) The complete amino acid sequence of a bacteriochlorophylla-protein fromProsthecochloris aestuarii. J Biol Chem 261: 3607–3615

    Google Scholar 

  • Dracheva S, Williams JC and Blankenship RE (1992) Cloning and sequencing of the FMO-protein gene fromChlorobium tepidum. In: Murata N (ed) Research in Photosynthesis, Vol I, pp 53–56. Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Fenna RE and Matthews BW (1975) Chlorophyll arrangement in a bacteriochlorophyll protein fromChlorobium limicola. Nature (London) 258: 573–588

    Google Scholar 

  • Fenna RE, Matthews BW, Olson JM and Shaw EK (1974) Structure of a bacteriochlorophyll-protein from the green photosynthetic bacteriumChlorobium limicola: Crystallographic evidence for a trimer. J Mol Biol 84: 231–240

    Google Scholar 

  • Gibson J (1961) Cytochrome pigments from the green photosynthetic bacteriumChlorobium thiosulphatophilum. Biochem J 79: 151–158

    Google Scholar 

  • Gray BH, Fowler CF, Nugent NA, Rigopoulos N and Fuller RC (1973) Reevaluation ofChloropseudomonas ethylica strain 2-K. Int J Syst Bacteriol 23: 256–264

    Google Scholar 

  • Larsen H (1952) On the culture and general physiology of the green sulfur bacteria. J Bacteriol 64: 187–196

    Google Scholar 

  • Matthews BW, Fenna RE, Bolognesi MC, Schmid MF and Olson JM (1979) Structure of a bacteriochlorophylla-protein from the green photosynthetic bacteriumProsthecochloris aestuarii. J Mol Biol 131: 259–285

    Google Scholar 

  • Olson JM (1966) Chlorophyll-protein complexes. Part II Complexes derived from green photosynthetic bacteria. In: Vernon LP and Seely GR (eds) The Chlorophylls, pp 413–425. Academic Press, New York

    Google Scholar 

  • Olson JM (1973) Historical note onChloropseudomonas ethylica strain 2K. Int J Syst Bacteriol 23: 265–266

    Google Scholar 

  • Olson JM (1978) Confused history ofChloropseudomonas ethylica 2K. Int J Syst Bacteriol 28: 128–129

    Google Scholar 

  • Olson JM and Romano CA (1962) A new chlorophyll from green bacteria. Biochim Biophys Acta 59: 726–728

    Google Scholar 

  • Olson JM, Filmer D, Radloff R, Romano CA and Sybesma C (1963) The protein-chlorophyll-770 complex from green bacteria. In: Gest H, San Pietro A and Vernon LP (eds) Bacterial Photosynthesis, pp 423–431. Antioch Press, Yellow Springs

    Google Scholar 

  • Olson JM, Koenig DF and Ledbetter MC (1969) A model of the bacteriochlorophyll-protein from green photosynthetic bacteria. Arch Biochem Biophys 129: 42–48

    Google Scholar 

  • Olson RA, Jennings WH and Olson JM (1969) Chlorophyll orientation in crystals of bacteriochlorophyll-protein from green photosynthetic bacteria. Arch Biochem Biophys 129: 30–41

    Google Scholar 

  • Philipson KD and Sauer K (1972) Exciton interaction in a bacteriochlorophyll-protein fromChloropseudomonas ethylica. Absorption and circular dichroism at 77 K. Biochemistry 11: 1880–1885

    Google Scholar 

  • Shaposhnikov VV, Kondratieva EN and Federov VD (1960) A new species of green sulphur bacteria. Nature (London) 187: 167–168

    Google Scholar 

  • Sybesma C and Olson JM (1963) Transfer of chlorophyll excitation energy in green photosynthetic bacteria. Proc Natl Acad Sci USA 49: 248–253

    Google Scholar 

  • Thornber JP and Olson JM (1968) The chemical composition of a crystalline bacteriochlorophyll-protein complex isolated from the green bacterium,Chloropseudomonas ethylicum. Biochemistry 7: 2242–2249

    Google Scholar 

  • Tronrud DE and Matthews BW (1993) Refinement of the structure of a water-soluble antenna complex from green photosynthetic bacteria by incorporation of the chemically determined amino acid sequence. In: Deisenhofer and Norris J (eds) The Photosynthetic Reaction Center Vol I, pp 13–21. Academic Press, San Diego

    Google Scholar 

  • Tronrud DE, Schmid MF and Matthews BW (1986) Structure and X-ray amino acid sequence of a bacteriochlorophylla protein fromProsthecochloris aestuarii refined at 1.9 Å resolution. J Mol Biol 188: 443–454

    Google Scholar 

  • VanNiel CB (1931) On the morphology and physiology of the purple and green sulphur bacteria. Arch Mikrobiol 3: 1–112

    Google Scholar 

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Olson, J.M. Reminiscence about‘Chloropseudomonas ethylicum’ and the FMO-protein. Photosynth Res 41, 3–5 (1994). https://doi.org/10.1007/BF02184138

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  • DOI: https://doi.org/10.1007/BF02184138

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