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Further experiments on the fixationin vitro of radiocalcium to sections of bone

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Résumé

L'auteur s'est proposé de préciser les conditions qui règlent les différences quantitatives de fixation du radiocalcium dans la structure des os à différents stades de formation. De minces lamelles d'os usées et polies ont été soumisesin vitro à des traitements divers (micro-incinération à 500° ou à 700°C, gabriélisation, décalcification partielle, traitement par la hyaluronidase, par l'acide phosphowolframique, etc.) et traitées en suite avec une faible solution de chlorure de Ca45. L'étude des autoradiographies démontre que seule la destruction totale des composants organiques de la matrice osseuse entraîne des modifications appréciables de la distribution du radiocalcium.

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References

  1. A. Engström andR. Amprino, Exper.6, 276 (1950);R. Amprino andA. Engström, Acta Anat.15, 1 (1952).

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  4. The degree of calcification of newly laid downHaversian systems is from 20 to 25% lower than that of old bone tissue. The progressive increment of calcification of recently deposited ground substance takes place at a very slow ratein vivo.R. Amprino andA. Engström, Acta Anat.15, 1 (1952).] The full calcification is attained in a period of at least a few months. [R. Amprino, Z. Zellforsch.37, 144 (1952).]

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  5. R. Amprino, Z. Zellforsch.37, 240 (1952); cf. alsoW. F. Neuman, M. W. Neuman, E. R. Main, andB. J. Mulryan, J. biol. Chem.179, 335 (1949);W. P. Norris andW. Kisieleski, Cold Spring Harbour Simp. Quant. Biol.13, 164 (1948).

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Authors and Affiliations

  1. Institute of Anatomy, University of Turin, Italy

    R. Amprino

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  1. R. Amprino
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Amprino, R. Further experiments on the fixationin vitro of radiocalcium to sections of bone. Experientia 8, 380–382 (1952). https://doi.org/10.1007/BF02176194

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  • DOI: https://doi.org/10.1007/BF02176194

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