Abstract
Gap junction channels have long been viewed as static structures containing a large-diameter, aqueous pore. This pore has a high permeability to hydrophilic molecules of ≈900 daltons in molecular weight and a weak ionic selectivity. The evidence leading to these conclusions is reviewed in the context of more recent observations primarily coming from unitary channel recordings from transfected connexin channels expressed in communication-deficient cell lines. What is emerging is a more diverse view of connexin-specific gap junction channel structure and function where electrical conductance, ionic selectivity, and dye permeability vary by one full order of magnitude or more. Furthermore, the often held contention that channel conductance and ionic or molecular selectivity are inversely proportional is refuted by recent evidence from five distinct connexin channels. The molecular basis for this diversity of channel function remains to be identified for the connexin family of gap junction proteins.
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References
Beblo, D. A., Wang, H.-Z., Westphale, E. M., Beyer, E. C., and Veenstra, R. D. (1994).Circulation 90, 1–359.
Bennett, M. V. L., Barrio, L. C., Bargiello, T. A., Spray, D. C., Hertzberg, E., and Saez, J. C. (1991).Neuron 6, 305–320.
Beyer, E. C., and Willecke, K. (1996). InAdvances in Molecular and Cell Biology, Vol. 18 (Hertzberg, E. L., ed.), JAI Press, Greenwich, Connecticut, in press.
Brink, P. R. (1991).J. Cardiovasc. Electrophysiol. 2, 360–366.
Brink, P. R., and Dewey, M. M. (1980).Nature 285, 101–102.
Brink, P. R., and Fan, S.-F. (1989).Biophys. J. 56, 579–593.
Brink, P. R., and Ramanan, S. V. (1985).Biophys. J. 48, 299–309.
Buehler, L. (1994).Science 265, 1018–1019.
Dahl, G., Miller, T., Paul, D., Voellmy, R., Werner, R. (1987).Science 236, 1290–1293.
Dahl, G., Nonner, W., and Werner, R. (1994).Biophys. J. 67, 1816–1822.
Dwyer, T. M., Adams, D. J., and Hille, B. (1980).J. Gen. Physiol. 75, 469–492.
Eghbali, J., Kessler, A., and Spray, D. C. (1990).Proc. Natl. Acad. Sci. USA 87, 1328–1331.
Elfgang, C., Eckert, R., Lichtenberg-Fraté, H., Butterweck, A., Traub, O., Klein, R. A., Hülser, D. F., and Willecke, K. (1995).J. Cell Biol. 129, 805–817.
Finkelstein, A. (1994).Science 265, 1017–1018.
Fishman, G. I., Spray, D. C., and Leinwand, L. A. (1990).J. Cell Biol. 111, 589–598.
Fishman, G. I., Moreno, A. P., Spray, D. C., and Leinwand, L. A. (1991).Proc. Natl. Acad. Sci. USA 88, 3525–3529.
Flagg-Newton, J., Simpson, I., and Loewenstein, W. R. (1979).Science 205, 404–407.
Hille, B. (1992).Ionic Channels of Excitable Membranes, Sinauer Associates Inc., Sunderland, Massachusetts.
Imanaga, I., Kameyama, M., and Irisawa, H. (1987).Am. J. Physiol. (Heart Circ. Physiol. 21) 252, H223-H232.
Imoto, K., Busch, C., Sakmann, B., Mishina, M., Konno, T., Nakai, J., Bujo, H., Mori, Y., Fukuda, K., and Numa, S. (1988).Nature 335, 645–648.
Imoto, K., Methfessel, C., Sakmann, B., Mishina, M., Mori, Y., Konno, T., Fukuda, K., Kurasaki, M., Bujo, H., Fujita, Y., and Numa, S. (1986).Nature 324, 670–674.
Levitt, D. G. (1975).Biophys. J. 15, 533–551.
Meister, M. (1994).Science 265, 1018.
Mishina, M., Tobimatsu, T., Imoto, K., Tanaka, K.-i., Fujita, Y., Fuduka, K., Kurasaki, M., Takahashi, H., Morimoto, Y., Hirose, T., Inayama, S., Takahashi, T., Kuno, M., and Numa, S. (1985).Nature 313, 364–369.
Neyton, J., and Trautmann, A. (1985).Nature 317, 331–335.
Renkin, E. M. (1955).J. Gen. Physiol. 38, 225–243.
Robinson, S. R., Hampson, E. C. G. M., Munro, M. N., and Vaney, D. I. (1993).Science 262, 1072–1074.
Safranyos, R. G. A., Caveney, S., Miller, J. G., and Peterson, N. O. (1987).Proc. Natl. Acad. Sci. USA 84, 2272–2276.
Schwarzmann, G., Weingandt, H., Rose, B., Zimmermann, A., Ben-Haim, D., and Loewenstein, W. R. (1981).Science 213, 551–553.
Simpson, I., Rose, B., and Loewenstein, W. R. (1977).Science 195, 294–296.
Spray, D. C., Moreno, A. P., Eghbali, B., Chanson, M., and Fishman, G. I. (1992).Biophys. J. 62, 48–50.
Stewart, W. W. (1978).Cell 14, 741–759.
Suchyna, T. M., Veenstra, R. D., Chilton, M., and Nicholson, B. J. (1994).Mol. Biol. Cell 5, 199a.
Unwin, N. (1993).J. Mol. Biol. 229, 1101–1124.
Veenstra, R. D., and Brink, P. R. (1992). InCell-Cell Interactions: A Practical Approach (Stevenson, B. R., Gallin, W. J., and Paul, D. L., eds.), IRL Press, Oxford, UK, pp. 167–201.
Veenstra, R. D., and DeHaan, R. L. (1986).Science 233, 972–974.
Veenstra, R. D., Wang, H.-Z., Westphale, E. M., and Beyer, E. C. (1992).Circ. Res. 71, 1277–1283.
Veenstra, R. D., Wang, H.-Z., Beyer, E. C., Ramanan, S. V., and Brink, P. R. (1994).Biophys. J. 68, 1915–1928.
Veenstra, R. D., Beblo, D. A., Wang, H.-Z., and Brink, P. R. (1995a).Mol. Biol. Cell 6, 190a.
Veenstra, R. D., Wang, H.-Z., Beblo, D. A., Chilton, M. G., Harris, A. L., Beyer, E. C., and Brink, P. R. (1995b)Circ. Res. 77, 1156–1165.
Verselis, V. K., and Veenstra, R. D. (1996). InAdvances in Molecular and Cell Biology, Vol. 18 (Hertzberg, E. L., ed.), JAI Press, Greenwich, Connecticut, in press.
Villaroel, A., and Sakmann, B. (1992).Biophys. J. 62, 196–205.
Villaroel, A., Herlitze, S., Witzemann, V. Koenen, M., and Sakmann, B. (1992).Proc. R. Soc. London B 249, 317–324.
Wang, F., and Imoto, K. (1992).Proc. R. Soc. London B 250, 11–17.
Wang, H.-Z., and Veenstra, R. D. (1995).Circulation 92, 1–40.
Wang, H.-Z., Li, J., Lemanski, L. F., and Veenstra, R. D. (1992).Biophys. J. 63, 139–151.
Willecke, K., Hennemann, H., Dahl, E., Jungbluth, S., and Heynkes, R. (1991).Eur. J. Cell Biol. 56, 1–7.
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Veenstra, R.D. Size and selectivity of gap junction channels formed from different connexins. J Bioenerg Biomembr 28, 327–337 (1996). https://doi.org/10.1007/BF02110109
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DOI: https://doi.org/10.1007/BF02110109