Abstract
Since recent studies have emphasized that mast cells from different tissues within a given species may exhibit marked differences in their functional properties, we have now examined the effect of some immunological and non-immunological histamine releasers on human mesenteric mast cells. The mesentery derived from the patients subjected to gall-bladder surgery was dispersed by collagenase (concentration of enzyme — 1 mg/ml, time of incubation — 90 min, 37°C). The mesenteric cell suspension contained about 2% mast cells as identified by staining with toluidine blue. We observed that the mesenteric mast cells released histamine when challenged with anti-human IgE, but marked individual variations were observed. These cells had a low sensitivity to challenge with Concanavalin A and compound 48/80 (histamine release about 6%), but responded to ionophore A23187 and polymyxin B (histamine release up to 24% and 22% respectively).
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This work was supported by Polish Academy of Sciences (Grant CPBP 06.01.).
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Brzezińska-Błaszczyk, E., Czuwaj, M. Isolation and sensitivity of human mesenteric mast cells to immunological and nonimmunological histamine releasers. Agents and Actions 20, 226–228 (1987). https://doi.org/10.1007/BF02074676
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DOI: https://doi.org/10.1007/BF02074676