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On the toxicity of low doses of tetrasodium-ethylenediamine-tetraacetate (Na-EDTA) in normal rat kidney (NRK) cells in culture

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Abstract

The toxicity of tetrasodium-ethylenediaminetetraacetate (Na-EDTA) was investigated in normal rat kidney cells (NRK-52E, American Type Culture Collection) in culture. Cell death and reduced colony-forming ability were observed at Na-EDTA concentrations <100 μM, that is at concentrations lower than that required to chelate all the Ca2+ in the growth medium. No toxicity was observed when cells were exposed to 100 μM Zn- or Fe-EDTA, but the same concentration of Cu-EDTA was as toxic as Na-EDTA. Continuous exposure of the cell cultures to 5 μM Na- or Zn-EDTA for up to 7 weeks yielded no indications of toxicity.

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Author notes

  1. Stephanie Hugenschmidt

    Present address: Institute for Applied Microbiology, University of Karlsruhe, W-7500, Karlsruhe, Germany

  2. David M. Taylor

    Present address: School of Chemistry, University of Wales College of Cardiff, P. O. Box 912, CF1 3TB, Cardiff, UK

Authors and Affiliations

  1. Institut für Genetik und Toxikologie, Kernforschungszentrum Karlsruhe, Postfach 3640, W-7500, Karlsruhe 1, Germany

    Stephanie Hugenschmidt, Felicitas Planas-Bohne & David M. Taylor

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  1. Stephanie Hugenschmidt
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  2. Felicitas Planas-Bohne
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  3. David M. Taylor
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Hugenschmidt, S., Planas-Bohne, F. & Taylor, D.M. On the toxicity of low doses of tetrasodium-ethylenediamine-tetraacetate (Na-EDTA) in normal rat kidney (NRK) cells in culture. Arch Toxicol 67, 76–78 (1993). https://doi.org/10.1007/BF02072040

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  • DOI: https://doi.org/10.1007/BF02072040

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