Abstract
Four synthetic multiring analogs of strigol, a naturally occurring sesquiterpene lactone that promotes germination of dormant seeds ofStriga (witchweed), were found to stimulate germination of dormantLactuca (lettuce) seeds. The effects on light-sensitive and light-insensitive lettuce seeds were concentration-dependent and exceeded those produced by equimolar (0.1 mM) solutions of gibberellic acid. Strigol and epistrigol promoted lettuce seed germination to a lesser degree than did the synthetic analogs. The strigol group compounds had minimal effect on the germination of monocot seeds. The results indicate that the synthetic strigol analogs have plant growth regulatory activity in dormant seeds of genera beyondStriga in which germination stimulation by strigol and the synthetic analogs was first demonstrated.
Similar content being viewed by others
References
Babiker AGT, Hamdoun AM (1982) Factors affecting the activity of GR7 of stimulating germination ofStriga hermontheca (Del.) Benth. Weed Res 22:111–116
Bewley JD, Black M (1982) Physiology and biochemistry of seeds, Vol 2: Viability, dormancy and environmental control. Springer-Verlag, Berlin, pp 186–192
Bradow JM (1985) Germination regulation byAmaranthus palmeri andAmbrosia artemisiifolia. In: Thompson AC (ed) The chemistry of allelopathy: Biochemical interactions among plants, ACS Symposium Series No. 268. American Chemical Society, Washington, pp 285–299
Bradow JM (1986) Germination promotion in dormant shepherdspurse (Capsella bursa-pastoris) seeds by strigol analogs and other stimulants. Weed Sci 34:1–7
Brooks DW, Kennedy E, Bevinakatti HS, Pepperman AB Jr, Bradow JM (1987) Synthetic studies of strigol and its analogs. In: Baker DR, Fenyes JG, Moberg WK, Cross B (eds) Synthesis and chemistry of agrochemicals, ACS Symposium Series No. 355. American Chemical Society, Washington, pp 433–444
Brown R, Edwards M (1945) Effects of thiourea and allylthiourea on the germination of the seed ofStriga lutea. Nature 155:455–456
Connick WJ, Pepperman AB (1981) Preparation, isolation, and high performance liquid chromatographic separation of diastereomers of a strigol analogue. Agric Food Chem 29:984–986
Cook CE, Whichard LP, Turner B, Wall ME, Egley GH (1966) Germination of witchweed (Striga lutea Lour.): Isolation and properties of a potent stimulant. Science 154:1189–1190
Cook CE, Whichard LP, Wall ME, Egley GH, Coggon P, Luhan PA, McPhail AT (1972) Germination stimulants. II. The structure of strigol. J Am Chem Soc 94:6198–6199
Heather JR, Mittal RSD, Sih CJ (1974) The total synthesis of racemic strigol. J Am Chem Soc 96:1976–1977
Heather JB, Mittal RSD, Sih CJ (1976) Synthesis of the witchweed germination stimulant (+)-strigol. J Am Chem Soc 98:3661–3669
Hsiao AI, Worsham AD, Moreland DE (1981) Regulation of witchweed (Striga asiatica). Conditioning and germination by dl-strigol. Weed Sci 29:101–104
Johnson AW, Rosebery G, Parker C (1976) A novel approach toStriga andOrobanche control using synthetic stimulants. Weed Res 16:223–227
Johnson AW, Gowda G, Hassanali A, Knox J, Manaco S, Rozavi A, Rosebery G (1981) The preparation of synthetic analogues of strigol. J Chem Soc Perkin Trans 1:1734–1743
Jones RL, Stoddart JL (1977) Gibberellins and seed germination. In: Khan AA (ed) The physiology and biochemistry of seed dormancy and germination. North Holland, Amsterdam, pp 77–109
Maguire JD, Overland A (1959) Laboratory germination of seeds of weedy and native plants. Washington State Agric Exp Sta Circ 49
Mayer AM, Poljakoff-Mayber A (1982) The germination of seeds, 3d Ed. Pergamon, Oxford, U.K., 211 pp
Mayer AM, Poljakoff-Mayber A, Zacks S (1958) The interaction of thiourea and ascorbic acid in their effect on germination and growth. Bull Res Counc Isr 6D:103–107
Pepperman AB, Connick WJ, Vail SL, Worsham AD, Pavlista AD, Moreland DE (1982) Evaluation of precursors in the synthesis of strigol analogs as witchweed seed germination stimulants. Weed Sci 30:561–566
Poljakoff-Mayber A, Mayer AM (1961) Effect of thiourea on seed germination. Ind J Plant Physiol 3:125–138
Reynolds T, Thompson PA (1973) Effects of kinetin, gibberellins and +/− abscisic acid on the germination of lettuce (Lactuca sativa). Physiol Plant 38:516–522
Simpson GM (1978) Metabolic regulation of dormancy in seeds. A case history of wild oat. In Clutter ME (ed) Dormancy and development arrest. Academic Press, New York, pp 167–220
Sokal RR, Rohlf FJ (1981) Biometry, 2nd Ed. Freeman, San Francisco, 859 pp
Steel RGD, Torrie JH (1980) Principles and procedures of statistics. McGraw-Hill, New York, pp 190–191
Steinbauer BP, Grigsby B, Correa L, Frank P (1955) A study of methods for obtaining laboratory germination of certain weeds seeds. Assoc Offic Seed Anal Proc 45:48–52
Stevens RA, Eplee RE (1979)Striga germination stimulants. In: Musselman LJ, Worsham AD, Eplee RE (eds) Proc 2d symposium on parasitic weeds. North Carolina State University Press, Raleigh, pp 211–214
Thomas TH (1977) Cytokinins, cytokinin-active compounds and seed germination. In: Khan AA (ed) The physiology and biochemistry of seed dormancy and germination. North-Holland, Amsterdam, pp 111–144
Wolfbeis OS, Junek H (1979) B,B-Diacyl-enamines and -enoles. III. Formylation of the CH2-acidic compounds via the anilinomethylene derivatives. Z Naturforsch B: Anorg Chem Org Chem 34B:283–289
Worsham AD, Moreland DE, Klingman GC (1959) Stimulation ofStriga asiatica (witchweed) seed germination by 6-substituted purines. Science 130:556–557
Yoshikawa F, Worsham AD, Moreland DE, Eplee RE (1978) Biochemical requirements for seed germination and shoot development of witchweed (Striga asiatica). Weed Sci 26:119–122 A0443012 00005 CS-SPJRNPDF [HEADSUP]
Author information
Authors and Affiliations
Additional information
Names of companies of commercial products are given solely for the purpose of providing specific information; their mention does not imply recommendation or endorsement by the U.S. Department of Agriculture over others not mentioned.
Rights and permissions
About this article
Cite this article
Bradow, J.M., Connick, W.J. & Pepperman, A.B. Comparison of the seed germination effects of synthetic analogs of strigol, gibberellic acid, cytokinins, and other plant growth regulators. J Plant Growth Regul 7, 227–239 (1988). https://doi.org/10.1007/BF02025265
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02025265