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Identification and quantitative analysis of gibberellins inCitrus

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Abstract

Nine gibberellins (GAs) have been identified from tissues of Valencia orange (Citrus sinensis Osbeck) using gas chromatography—mass spectrometry and gas chromatography-selected ion monitoring of high-performance liquid chromatography (HPLC)-fractionated extracts. These GAs are GA1, GA3, GA8, GA19, GA20, GA29, 3-epi-GA1, 2-epi-GA29, and iso-GA3. Selected-ion monitoring and stable-isotope dilution assays have been used to estimate levels of some of these GAs in vegetative and reproductive tissues. GA29 was found to be the most abundant GA measured. GA1 was found in all samples examined, and there was always less 3-epi-GA1 than GA1. GA20 was present in most extracts. Leaves of developing inflorescence shoots contained six times more GA29 than did leaves of comparable vegetative shoots. Levels of GA29 increased during the early stages of fruit development. GA20 may be more abundant in growing fruitlets than in those about to abscise; however, there were no consistent differences in the relative amounts of the other GAs. No major differences were found between tissues of immature seeded and seedless fruit, and developing seeds did not contain high levels of any of the GAs measured. It is concluded that seed-produced GAs are not essential for normal fruit development in Valencia orange.

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References

  • Agusti M, Garcia-Mari F, Guardiola JL (1982) Gibberellic acid and fruit set in sweet orange. Sci Hortic 17:257–264

    Article  CAS  Google Scholar 

  • Aron Y, Monselise SP, Costo J (1985) Chemical control of vegetative growth in citrus trees by paclobutrazol. Hort Sci 20:96–98

    CAS  Google Scholar 

  • Crozier A (ed) (1985) The biochemistry and physiology of gibberellins. vol. 1. Praeger, New York

    Google Scholar 

  • Garcia-Luis A, Almela V, Monerri C, Agusti M, Guardiola JL (1986) Inhibition of flowering in vivo by existing fruits and applied growth regulators inCitrus unshiu. Physiol Plant 66:515–520

    Article  CAS  Google Scholar 

  • Gaskin P, Gilmour SJ, MacMillan J, Sponsel VM (1985) Gibberellins in immature seeds and dark-grown shoots ofPisum sativum. Gibberellins identified in the tall cultivar Alaska in comparison with those in the dwarf Progress No. 9. Planta 163:283–289

    Article  CAS  Google Scholar 

  • Gaskin P, MacMillan J, Firn RD, Pryce RJ (1971) “Parafilm:” a convenient source ofn-alkane standards for the determination of gas chromatographic retention indices. Phytochemistry 10:1155–1157

    Article  CAS  Google Scholar 

  • Goldschmidt EE (1976) Endogenous growth substances of citrus tissues. Hort Sci 11:95–99

    CAS  Google Scholar 

  • Goldschmidt EE, Goren R, Even-Chen Z, Bittner S (1973) Increase in free and bound abscisic acid during natural and ethylene-induced senescence of citrus fruit peel. Plant Physiol 51:879–882

    Article  PubMed  CAS  Google Scholar 

  • Igoshi M, Yamaguchi I, Takahashi N, Hirose K (1971) Plant growth substances in the young fruit ofCitrus unshiu. Agric Biol Chem 35:629–631

    CAS  Google Scholar 

  • Ingram TJ, Reid JB, Murfet IC, Gaskin P, Willis CL, MacMillan J (1984) Internode length inPisum. TheLe gene controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160:455–463

    Article  CAS  Google Scholar 

  • Kamiya Y, Graebe JE (1983) The biosynthesis of all major pea gibberellins in a cell-free system fromPisum sativum. Phytochemistry 22:681–689

    Article  CAS  Google Scholar 

  • Kawarada A, Sumiki V (1959) The occurrence of gibberellin A1 in water sprouts of citrus. Bull Agric Chem Soc Japan 23:343–344

    CAS  Google Scholar 

  • Kovats E (1958) Gas chromatographische Charakterisierung organischer Verbindungen. I. Retentions indices aliphatischer halogenide, alkohole, aldehyde und ketone. Helv Chim Acta 41:1915–1932

    Article  CAS  Google Scholar 

  • Lord EM, Eckard KJ (1987) Shoot development inCitrus sinensis L. (Washington Navel orange). II. Alteration of developmental date of flowering shoots after GA3 treatment. Bot Gaz 148: 17–22

    Article  CAS  Google Scholar 

  • Monselise SP, Brosh P, Costo J (1981) Off-season bloom in Temple orange repressed by gibberellin. Hort Sci 16:786

    CAS  Google Scholar 

  • Moore PH, Pharis RP, Koshioka M (1986) Gibberellins in apical shoot meristems of flowering and vegetative sugarcane. J Plant Growth Regul 5:101–109

    Article  CAS  Google Scholar 

  • Moss GI (1970) Fruit-set in sweet orange (Citrus sinensis): the influence of inflorescence leaves. Phyton 27:141–148

    Google Scholar 

  • Moss GI (1972) Promoting fruit-set and yield in sweet orange using plant growth substances. J Exp Agric Animal Husbandry 12:96–102

    Article  CAS  Google Scholar 

  • Muller IA, Young MJ (1982) Influence of gibberellic acid and effectiveness of several carriers on growth of sour orange (Citrus aurantium L.) seedlings. Hort Sci 17:673–674

    CAS  Google Scholar 

  • Nadeau R, Rappaport L (1974) The synthesis of [3H]gibberellin A3 and [3H]gibberellin A1 by the palladium catalysed actions of carrier-free tritium on gibberellin A3. Phytochemistry 13:1537–1545

    Article  CAS  Google Scholar 

  • Phinney BO, Freeling M, Robertson DS, Spray CR, Silverthorne J (1986) Dwarf mutants in maize —the gibberellin biosynthetic pathway and its molecular future. In: Bopp M (ed) Plant growth substances 1985. Springer-Verlag, Berlin, pp. 55–64

    Google Scholar 

  • Pryce RJ (1973) Decomposition of aqueous solutions of gibberellic acid on autoclaving. Phytochemistry 12:507–514

    Article  CAS  Google Scholar 

  • Randhawa GS, Singh JP (1986) Growth response of citrus seedling rootstocks in response to gibberellic acid. Ind J Hort 55:75–78

    Google Scholar 

  • Reeve DR, Crozier A (1975) Gibberellin bioassays. In: Krishnamoorthy HN (ed) Gibberellins and plant growth. Wiley Eastern, New Delhi, pp. 35–64

    Google Scholar 

  • Southwick SM, Davies FS (1982) Growth regulator effects on fruit set and fruit size in Navel orange. J Am Soc Hortic Sci 107:395–397

    CAS  Google Scholar 

  • Sponsel VM (1983) In vivo gibberellin metabolism in higher plants. In: Crozier A (ed) The biochemistry and physiology of gibberellins. vol. 1. Praeger, New York, pp. 151–250

    Google Scholar 

  • Spray C, Phinney BO, Gaskin P, Gilmour SJ, MacMillan J (1984) Internode length inZea mays L. The dwarf-1 mutation controls the 3β-hydroxylation of gibberellin A20 to gibberellin A1. Planta 160:464–468

    Article  CAS  Google Scholar 

  • Turnbull CGN (1988) Gibberellins and control of fruit retention and seedlessness in Valencia orange. Acta Hortic (in press)A0443016 00004 CS-SPJRNPDF [HEADSUP]

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  1. CSIRO Division of Horticulture, St. Lucia, 4067, Brisbane, Queensland, Australia

    C. G. N. Turnbull

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  1. C. G. N. Turnbull
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Turnbull, C.G.N. Identification and quantitative analysis of gibberellins inCitrus . J Plant Growth Regul 8, 273–282 (1989). https://doi.org/10.1007/BF02021820

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  • DOI: https://doi.org/10.1007/BF02021820

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