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Gibberellins in immature seeds and dark-grown shoots of Pisum sativum

Gibberellins identified in the tall cultivar Alaska in comparison with those in the dwarf Progress No. 9

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Abstract

Gibberellins (GAs) A17, A19, A20, A29, A44, 2βOH-GA44 (tentative) and GA29-catabolite were identified in 21-day-old seeds of Pisum sativum cv. Alaska (tall). These GAs are qualitatively similar to those in the dwarf cultivar Progress No. 9 with the exception of GA19 which does not accumulate in Progress seeds. There was no evidence for the presence of 3-hydroxylated GAs in 21 day-old Alaska seeds. Dark-grown shoots of the cultivar Alaska contein GA1, GA8, GA20, GA29, GA8-catabolite and GA29-catabolite. Dark-grown shoots of the cultivar Progress No.9 contain GA8, GA20, GA29 and GA29-catabolite, and the presence of GA1 was strongly indicated. Quantitation using GAs labelled with stable isotope showed the level of GA1 in dark-grown shoots of the two cultivars to be almost identical, whilst the levels of GA20, GA29 and GA29-catabolite were significantly lower in Alaska than in Progress No. 9. The levels of these GAs in dark-grown shoots were 102- to 103-fold less than the levels in developing seeds. The 2-epimer of GA29 is present in dark-grown-shoot extracts of both cultivars and is not thought to be an artefact.

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Abbreviations

cv:

cultivar

GAn :

gibberellin An

GC:

gas chromatography

GC-MS:

combined gas chromatographymass spectrometry

HPLC:

high-pressure liquid chromatography

KRI:

Kovats retention index

MeTMSi:

methyl ester trimethylsilyl ether

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Authors and Affiliations

  1. Agricultural and Food Research Council Research Group, School of Chemistry, University of Bristol, BS8 1TS, Bristol, UK

    Paul Gaskin, Sarah J. Gilmour, Jake MacMillan & Valerie M. Sponsel

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  1. Paul Gaskin
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  2. Sarah J. Gilmour
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  3. Jake MacMillan
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  4. Valerie M. Sponsel
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Gaskin, P., Gilmour, S.J., MacMillan, J. et al. Gibberellins in immature seeds and dark-grown shoots of Pisum sativum . Planta 163, 283–289 (1985). https://doi.org/10.1007/BF00393519

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  • DOI: https://doi.org/10.1007/BF00393519

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