Abstract
Bone growth and calcium metabolism were studied in marine acellular-boned toadfish (Opsanus tau) by tetracycline and by45Ca labeling over a 26-day period. The uptake of tracer in vertebrae was initially faster than in the jaws, but the percent retention of45Ca on a gram ash basis in these tissues was not significantly different after 4–6 hours. This was the time at which microdensitometric analysis of contact autoradiographs of jaw sections indicated that peak45Ca uptake had occurred on all cortical bone surfaces. No loss of45Ca from bone surfaces was detected during the next 14 days. The differences in45Ca uptake in these tissues appeared to be related to the relative surface areas of bone rather than to differences in mineral content per unit volume of bone. Attempts to measure appositional bone growth by the separation of two tetracycline labels administered at an interval of 18 days were unsuccessful, and only a single subperiosteal fluorescent band 8–9 μ thick was observed. This was probably the result of a very low rate of excretion of tetracycline from the blood, together with a very low appositional rate of bone growth of only 0.2–0.3 μ/day. The fall in the plasma radioactivity was almost inversely proportional to time since injection. No diffuse labeling could be detected, due presumably to the absence of osteocytes, in lacunae and canaliculi which, in cellular bone, permits diffusion of calcium ions from extracellular fluid to bone crystals deep within the skeleton. The diffuse component, if present, was less than one-fifth that expected in cellular bone.
Résumé
La croissance osseuse et le métabolisme du calcium sont étudiés chezOpsanus tau marin, à os acellulaire, à l'aide de marquages à la tétracycline et45Ca, injectés par voie intra-musculaire pendant 26 jours. La localisation du marquage au niveau des vertèbres est initialement plus rapide au niveau des maxillaires, mais le pourcentage de rétention du45Ca au niveau de ces tissus, en se basant sur des grammes de cendres, n'est pas significativement différent après 4–6 heures. C'est le temps au bout duquel l'analyse microdensitométrique d'autoradiographies par contact des coupes de maxillaires indique un maximum d'absorption de45Ca au niveau de toutes les surfaces corticales osseuses. Aucune perte de45Ca des surfaces osseuses n'est mise en évidence durant les 14 jours suivants. Les différences d'absorption du45Ca dans ces tissus semblent liées aux surfaces osseuses relatives, plutôt qu'aux différences du contenu minéral par unité de volume d'os. Des essais de mesure de l'apposition osseuse par séparation de deux injections de tétracycline, à des intervalles de 18 jours, furent réalisés sans succès et une seule bande fluorescente sous-périostée, de 8–9 μ de large a été observée. Ce fait est probablement lié à la vitesse d'excrétion sanguine très lente de l'os, d'environ 0.2–0.3 μ par jour. La chute de la radioactivité du plasma est presqu'inversement proportionnelle au temps après injection. Aucun marquage diffus n'a pu être localisé, sans doute, par suite de l'absence d'ostéocytes, de lacunes et de canalicules qui, dans l'os cellulaire, facilite la diffusion des ions calciques du liquide extracellulaire vers les cristaux osseux situés profondément dans le squelette. Le composé diffus, s'il est présent, constitue moins d'un cinquième de ce que l'on s'attend à trouver dans l'os cellulaire.
Zusammenfassung
Das Knochenwachstum und der Calciummetabolismus wurden beim knochenzellosen marinen Krötenfisch (Opsanus tau) während 26 Tagen anhand von Tetracyclin- und45Ca-Markierung verfolgt. Die Aufnahme des Isotopes erfolgte vorerst rascher in den Wirbelkörpern als in den Kieferknochen, jedoch war die prozentuale Retention von45Ca, auf das Aschegewicht bezogen, in diesen Geweben nach 4–6 Std nicht signifikant verschieden. Nach dieser Zeit konnte anhand von mikrodensitometrischen Untersuchungen von Kieferschnitt-Autoradiographien festgestellt werden, daß in allen Corticalis-Oberflächen die maximale45Ca-Aufnahme stattgefunden hatte. Während der folgenden 14 Tage konnte kein45Ca-Verlust von den Knochenoberflächen nachgewiesen werden. Die Unterschiede der45Ca-Aufnahme in diesen Geweben scheinen eher mit der relativen Oberflächengröße der Knochen als mit dem Mineralgehalt pro Knochenvolumen in Zusammenhang zu stehen. Versuche, die angelagerte Knochenschicht durch zwei, im Abstand von 18 Tagen vorgenommene Tetracyclin-Markierungen zu messen, verliefen ergebnislos; es konnte nur eine einzige 8–9 μ dicke, subperiostale Fluoreszenzschicht beobachtet werden. Dies war vermutlich einer sehr langsamen Abnahme des Tetracyclins im Blut, verbunden mit einer stark verlangsamten Knochenbildung von nur 0,2–0,3 μ pro Tag zuzuschreiben. Die Abnahme der Radioaktivität im Plasma war beinahe umgekehrt proportional zur Zeitspanne seit der Injektion. Dies ist vermutlich auf das Fehlen von Osteocyten, Lacunae und Canaliculi zurückzuführen, die ja im zellulären Knochen eine Diffusion von Calciumionen aus der Extrazellulärflüssigkeit zu den tiefer im Skelett liegenden Knochenkristallen ermöglichen. Wenn eine diffuse Komponente vorlag, so betrug sie weniger als ⅕ der für zellulären Knochen erwarteten.
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Simmons, D.J., Simmons, N.B. & Marshall, J.H. The uptake of calcium-45 in the acellular-boned toadfish. Calc. Tis Res. 5, 206–221 (1970). https://doi.org/10.1007/BF02017550
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DOI: https://doi.org/10.1007/BF02017550