Skip to main content
Log in

Production of enterotoxin byEscherichia coli at four, twenty-two and thirty-seven degrees centigrade

  • Published:
European Journal of Clinical Microbiology Aims and scope Submit manuscript

Abstract

One hundred and seventy-sevenEscherichia coli strains isolated from food, pigs and humans were tested for the production of heat-labile and heat-stable enterotoxin at 4, 22, and 37 °C. Heat-labile enterotoxin was detected in culture supernatants by an enzyme-linked immunosorbent assay, and heat-stable enterotoxin by the infant mouse bioassay. Thirty strains produced heat-labile enterotoxin, and twenty heat-stable enterotoxin. None of the strains isolated from food were enterotoxigenic. Fifty-seven per cent of the human and porcine strains producing heat-labile enterotoxin at 37 °C also produced the toxin at 4 °C. The fact thatEscherichia coli enterotoxin may be present in food at consumption must be considered pathogenetically relevant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Dupont, H. L., Formal, S. B., Hornic, R. B., Snyder, M. J., Labonati, J. P., Sheahan, D. C., LaBrec, E. H., Kalas, J. P.: Pathogenesis ofEscherichia coli diarrhea. New England Journal of Medicine 1971, 285: 1–9.

    PubMed  Google Scholar 

  2. Fantasia, L. D., Mestrandrea, L., Schrade, J. P., Yager, J.: Detection and growth of enteropathogenicEscherichia coli in soft ripened cheese. Applied Microbiology 1975, 29: 179–185.

    PubMed  Google Scholar 

  3. Jiwa, S. F. H., Krovacek, T., Wadström, T.: Enterotoxigenic bacteria in food and water from an Ethiopian community. Applied and Environmental Microbiology 1981, 41: 1010–1019.

    PubMed  Google Scholar 

  4. Möllby, R., Hansson, N., Brag, H., Danielsson, M.-L., Jonsson, P., Olsson, E., Wadström, T.:Escherichia coli — en patogen hygienindikator i livsmedel? Svensk Veterinärtidning 1978, 30: 271–275.

    Google Scholar 

  5. Rosenberg, M. L., Koplan, J. P., Wachsmuth, I. K., Wells, J. G., Gangarosa, E. J., Guerrant, R. L., Sack, D. A.: Epidemic diarrhea at Crater Lake from enterotoxigenicEscherichia coli. Annals of Internal Medicine 1977, 86: 714–718.

    PubMed  Google Scholar 

  6. Sack, R. B., Sack, D. A., Mehlman, I. J., Ørskov, F., Ørskov, I.: EnterotoxigenicEscherichia coli isolated from food. Journal of Infectious Diseases 1977, 135: 313–317.

    PubMed  Google Scholar 

  7. Kapperud, G.: Studies on the pathogenicity ofYersinia enterocolitica andY. enterocolitica-like bacteria. 1. Enterotoxin production at 22 °C and 37 °C by environmental and human isolates from Scandinavia. Acta Pathologica et Microbiologica Scandinvica Section B 1980, 88: 287–291.

    Google Scholar 

  8. Kapperud, G., Langeland, G.: Enterotoxin production at refrigeration temperature byYersinia enterocolitica andY. enterocolitica-like bacteria. Current Microbiology 1981, 5: 119–122.

    Google Scholar 

  9. Yolken, R. H., Greenberg, H. B., Merson, M. H., Sack, R. B., Kapikan, A. L.: Enzyme-linked immunosorbent assay for detection ofEscherichia coli heat-labile enterotoxin. Journal of Clinical Microbiology 1977, 6: 439–444.

    PubMed  Google Scholar 

  10. Evans, D. G., Evans, D. J., Pierce, N. F.: Differences in the response of rabbit small intestine to heat-labile and heat-stable enterotoxins fromEscherichia coli. Infection and Immunity 1973, 7: 873–880.

    PubMed  Google Scholar 

  11. Dean, A. G., Ching, Y., Williams, R. G., Harden, L. B.: Test forEscherichia coli enterotoxin using infant mice. Application in a study of diarrhea in children in Honolulu. Journal of Infectious Diseases 1971, 125: 407–411.

    Google Scholar 

  12. Maurer, R. H.: Disc electrophoresis and related techniques of polyacrylamide gel electrophoresis. Walter de Gruyter, New York, 1971, p. 29–31.

    Google Scholar 

  13. Bäck, E., Blomberg, S., Wadström, T.: EnterotoxigenicEscherichia coli in Sweden. Infection 1971, 5: 2–5.

    Google Scholar 

  14. Goldhar, J., Peri, R., Zilberberg, R., Lahav, M.: EnterotoxigenicEscherichia coli (ETEC) isolated in Tel-Aviv (Israel) area. Medical Microbiology and Immunology 1980, 169: 53–61.

    PubMed  Google Scholar 

  15. Marier, R., Wells, J. G., Swanson, R. C., Calliahan, W., Mehlman, I. J.; An outbreak of enteropathogenicEscherichia coli foodborne disease traced to imported Trench cheese. Lancet 1973, ii: 1376–1378.

    Google Scholar 

  16. Schroeder, S. A., Caldwell, J. R., Veron, T. M., White, P. C., Granger, S. I., Bennett, J. V.: A waterborne outbreak of gastroenteritis in adults associated withEscherichia coli. Lancet 1968, i: 737–740.

    Google Scholar 

  17. Benoki, M.: Perspective on the food poisoning in Japan. In: Fukumi, H., Ohashi, M., Murakami, H., Kudoh, Y., Nakamura, A. (ed.): Gastrointestinal Infections in South-East Asia. III. Food Hygiene in the Problem of Gastrointestinal Infections. Southeast Asian Medical Information Center, Tokyo, 1978, p. 8–14.

    Google Scholar 

  18. Caseman, E. P.: Staphylococcal enterotoxin. Annals of the New York Academy of Science 1965, 28: 124–131.

    Google Scholar 

  19. Skjelkvåle, R., Duncan, C. L.: Enterotoxin formation by different toxigenic types ofClostridium perfringens. Infection and Immunity 1975, 11: 563–575.

    PubMed  Google Scholar 

  20. Evans, D. G., Satterwhite, T. K., Evans, D. J., Dupont, H. L.: Difference in serological responses and excretion patterns of volunteers challenged with enterotoxigenicEscherichia coli with and without the colonization factor antigen. Infection and Immunity 1978, 19: 883–888.

    PubMed  Google Scholar 

  21. Isascson, R. E., Fusco, P. C., Brinton, C. C., Moon, H. W.: In vitro adhesion ofEscherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors. Infection and Immunity 1978, 21: 392–397.

    PubMed  Google Scholar 

  22. Smyth, C. J., Olsson, E., Moncalvo, C., Söderlind, O., Ørskov, F., Ørskov, I.: K99 antigen-positive enterotoxigenicEscherichia coli from piglets with diarrhea in Sweden. Journal of Clinical Microbiology 1981, 13: 252–257.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Olsvik, Ø., Hushovd, O.T., Berdal, B.P. et al. Production of enterotoxin byEscherichia coli at four, twenty-two and thirty-seven degrees centigrade. Eur. J, Clin. Microbiol. 1, 12–16 (1982). https://doi.org/10.1007/BF02014134

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02014134

Keywords

Navigation