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Use of membrane filters for the enumeration of autotrophic thiobacilli

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Abstract

A new membrane filter technique for field use was developed for the enumeration of either aerobic or anaerobic, autotrophic, sulfur-oxidizing bacteria in waters and soils. Immediately after collection, samples were filtered through sulfur-coated filters and incubated in selective media. Acidification or gas evolution was used as a growth indicator of aerobic and anaerobic thiobacilli, respectively, and related to the initial number of cells deposited on the filter.

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References

  1. Augier, J. 1956. A propos de la numération des Azotobacter en milieu liquide,Ann. Inst. Pasteur 91, 759–765.

    Google Scholar 

  2. Baalsrud, K. and Baalsrud, K.S. 1954. Studies onThiobacillus Denitrificans, Arch. Mikrobiol. 20, 34–62.

    PubMed  Google Scholar 

  3. Baas-Becking, L.G.M. and Wood, F.E.J. 1955. Biological processes in estuarine environment. I: Ecology of the sulphur cycle,Proc. K. Ned. Akad. Wet. 58: 160–181.

    Google Scholar 

  4. Baldensperger, J. and Garcia, J.L. 1975. Reduction of oxidized inorganic nitrogen componds by a new strain ofThiobacillus denitrificans, Arch. Microbiol. 103: 31–36.

    PubMed  Google Scholar 

  5. Baldensperger, J. Guarraia, L.J. and Humphreys, W.J. 1974. Scanning electron microscopy of thiobacilli grown on colloidal sulfur.Arch. Microbiol. 99:323–329.

    PubMed  Google Scholar 

  6. Buchanan, R.E. and Gibbons, N.E. 1974.Bergey's Manual of Determinative Bacteriology (eighth ed) Williams and Wilkins, Baltimore.

    Google Scholar 

  7. Cook, T.M. 1964. Growth ofThiobacillus thiooxidans in shaken cultures,J. Bacteriol. 88: 620–623.

    PubMed  Google Scholar 

  8. Freney, J.R. 1967. Oxidation of sulphur in soils,Miner. Deposita 2: 181–187.

    Google Scholar 

  9. Hart, M.G.R. 1959. Sulphur oxidation in tidal mangrove soils of Sierra Leone,Plant Soil 11: 215–236.

    Google Scholar 

  10. Hattori, T. 1974.Microbial Life in the Soil, An Introduction. Marcel Dekker, New York.

    Google Scholar 

  11. Hutchinson, M., Johnstone, K.I. and White, D. 1969. Taxonomy of the genusThiobacillus: the outcome of numerical taxonomy applied to the group as a whole,J. Gen. Microbiol. 57: 397–410.

    PubMed  Google Scholar 

  12. Kodama, A. and Mori, T. 1968. Studies on the metabolism of a sulfur oxidizing bacterium. V. Comparative studies on sulfur and sulfite oxidizing systems ofThiobacillus thiooxidans, Plant Cell Physiol. 9: 725–734.

    Google Scholar 

  13. LaRivière, J.W.M. 1966. The microbial sulfur cycle and some of its implications for the geochemistry of sulfur isotopes,Geol. Rundschau 55: 568–582.

    Google Scholar 

  14. Moser, U.S. and Olson, R.V. 1953. Sulfur oxidation in four soils as influenced by soil moisture tension and sulfur bacteria,Soil Sci. 76: 251–257.

    Google Scholar 

  15. Mouraret, M. 1971. Etude biologique des eaux du barrage d'Ayame I en Côte d'Ivoire. Convention report ORSTOM-EECI, ORSTOM, Paris.

    Google Scholar 

  16. Mouraret, M. 1972. Etude biologique des eaux dans quelques cours d'eau du Cameroun et du Gabon. Convention report ORSTOM-S.N.E.C.-S.E.E.G. ORSTOM, Paris.

    Google Scholar 

  17. Pochon, J. and Tardieux, P. 1962.Techniques d'analyse en microbiologie du sol. Editions La Tourelle, Saint-Mondé France.

  18. Postgate, J.R. 1966. Media for sulphur bacteria,Lab. Pract. 15: 1239–1244.

    PubMed  Google Scholar 

  19. Spurny, M., Dostalek, M. and Ulehla, J. 1957. A method of quantitative determination of sulphate reducing bacteria,Folia Biol. (Praha) 3: 202–211.

    Google Scholar 

  20. Starkey, R.L. 1950. Relations of microorganisms to transformations of sulfur in soils,Soil Sci. 70: 55–65.

    Google Scholar 

  21. Starkey, R.L. 1966. Oxidation and reduction of sulfur compounds in soils,Soil Sc. 101: 297–306.

    Google Scholar 

  22. Stotzky, G. and Rem, L.T. 1966. Influence of clay minerals on microorganisms. I. Montmorillonite and kaolinite on bacteria,Can. J. Microbiol. 12: 547–563.

    PubMed  Google Scholar 

  23. Taylor, B.F. 1968. Oxidation of elemental sulfur by an enzyme system fromThiobacillus neapolitanus, Biochim. Biophys. Acta 170: 112–122.

    Google Scholar 

  24. Tilton, R.C., Cobet, A.B. and Jones, O.E. 1967. Marine Thiobacilli. I. Isolation and distribution,Can. J. Microbiol 13: 1521–1528.

    PubMed  Google Scholar 

  25. Tuovinen, O.H. and Kelly, D.P. 1973. Studies on the growth ofThiobacillus ferrooxidans. I. Use of Membrane filters and ferrous iron agar to determine viable numbers, and comparison with14CO2 fixation and iron oxidation as measures of growth,Arch. Mikrobiol. 88: 285–298.

    PubMed  Google Scholar 

  26. Tuovinen, O.H., Niemelä, S.I. and Gyllenberg, H.G. 1971. Tolerance ofThiobacillus ferrooxidans to some metals.Antonie van Leeuwenhoek 37: 489–496.

    PubMed  Google Scholar 

  27. Vishniac, W. and Santer, M. 1957. The Thiobacilli.Bacteriol. Rev. 21: 195–213.

    Google Scholar 

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Mouraret, M., Baldensperger, J. Use of membrane filters for the enumeration of autotrophic thiobacilli. Microb Ecol 3, 345–358 (1977). https://doi.org/10.1007/BF02010741

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