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Enrichment of Uncommon Bacteria in Soil by Fractionation Using a Metal Mesh Device

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Abstract

The use of a metal mesh device (MMD) as a precision bacterial separation filter is described. The MMD uses a structure in which identically shaped pores are arranged in a thin metal membrane. Four types of MMD with different pore sizes were used to fractionate bacteria in two types of soil. Through metagenomic analysis, the distribution of bacteria in the soil samples and in each MMD fraction was examined. In addition, eight types of previously described organic compounddegrading bacteria were used to evaluate the method, and changes in their composition following MMD fractionation were investigated. It was found that MMD fractions were enriched for all eight bacteria when compared with the initial sample. These results suggest that bacterial fractionation using MMD can enrich bacteria occurring at low frequencies in environmental samples.

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References

  1. T. Kondo, S. Kamba, K. Takigawa, T. Suzuki, Y. Ogawa, and N. Kondo, Procedia Eng., 2011, 25, 916.

    Article  Google Scholar 

  2. F. Miyamaru, S. Hayashi, C. Otani, K. Kawase, Y. Ogawa, H. Yoshida, and E. Kato, Opt. Lett., 2006, 31, 1118.

    Article  CAS  PubMed  Google Scholar 

  3. H. Seto, C. Yamashita, S. Kamba, T. Kondo, M. Hasegawa, M. Matsuno, Y. Ogawa, Y. Hoshino, and Y. Miura, Langmuir, 2013, 29, 9457.

    Article  CAS  PubMed  Google Scholar 

  4. H. Seto, S. Kamba, T. Kondo, Y. Ogawa, Y. Hoshino, and Y. Miura, Chem. Lett., 2014, 43, 408.

    Article  CAS  Google Scholar 

  5. X. Yin, K. Yamamoto, E. A. Wandera, Y. Ichinose, S. Kanba, T. Kondo, and M. Hasegawa, 2018 IEEE SENSORS Proceedings, 2018, New Delhi, India.

    Google Scholar 

  6. W. Whitman, D. Coleman, and W. Wiebe, Acad. Sci. USA, 1998, 95, 6578.

    Article  CAS  Google Scholar 

  7. S. Yoshida, K. Hiraga, T. Takehana, I. Taniguchi, H. Yamaji, Y. Maeda, K. Toyohara, K. Miyamoto, Y. Kimura, and K. Oda, Science, 2016, 351, 1196.

    Article  CAS  PubMed  Google Scholar 

  8. H. Seto, A. Saiki, S. Kamba, T. Kondo, M. Hasegawa, Y. Miura, Y. Hoshino, and H. Shinto, Anal. Sci., 2019, 35, 619.

    Article  CAS  PubMed  Google Scholar 

  9. E. Bolyen, J. R. Rideout, M. R. Dillon, N. A. Bokulich, C. C. Abnet, G. A. Al-Ghalith, H. Alexander, E. J. Alm, M. Arumugam, F. Asnicar, Y. Bai, J. E. Bisanz, K. Bittinger, A. Brejnrod, C. J. Brislawn, C. T. Brown, B. J. Callahan, A. M. Caraballo-Rodriguez, J. Chase, E. K. Cope, {etet al.} Nat. Biotechnol., 2019, 37, 852.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. B. H. Kaplan and E. R. Stadtman, J. Biol. Chem., 1968, 243, 1787.

    Article  CAS  PubMed  Google Scholar 

  11. D. Inoue, T. Tsunoda, K. Sawada, N. Yamamoto, Y. Saito, K. Sei, and M. Ike, Biodegradation, 2016, 27, 277.

    Article  CAS  PubMed  Google Scholar 

  12. M. Takeo, M. Murakami, S. Niihara, K. Yamamoto, M. Nishimura, D. Kato, and S. Negoro, J. Bacterial., 2008, 190, 7367.

    Article  CAS  Google Scholar 

  13. K. Nochi, M. Otsuka, H. Tsuda, S. Koshimizu, K. Igarashi, and H. Kato, Institute of Architectural Environmental Engineering Report, Kanto Gakuin University, 2008, 31, 1.

    Google Scholar 

  14. K. Ohtaguchi and T. Yokoyama, Energy Convers. Manag., 1997, 38, 539.

    Article  Google Scholar 

  15. A. R. J. Curson, M. J. Sullivan, J. D. Todd, and A. W. B. Johnson, ISME L, 2011, 5, 1191.

    Article  CAS  Google Scholar 

  16. T. Suzuki, Y. Ichihara, M. Yamada, and K. Tomomura, Agric. Biol. Chem., 1973, 37, 747.

    CAS  Google Scholar 

  17. Y. J. Wu, L. M. Whang, S. J. Huang, C. N. Lei, and S. S. Cheng, Water Sci. Technol., 2008, 58, 1085.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Murata Manufacturing Co., Ltd., 1-10-1 Higashikotari, Nagaokakyo, Kyoto, 617-8555, Japan

    Seiji Kamba

  2. Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga, 526-0829, Japan

    Atsushi Ogura & Makoto Hasegawa

  3. Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi, Fukuoka, 819-0395, Japan

    Yoshiko Miura

Authors
  1. Seiji Kamba
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  2. Atsushi Ogura
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  3. Yoshiko Miura
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  4. Makoto Hasegawa
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Correspondence to Makoto Hasegawa.

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Kamba, S., Ogura, A., Miura, Y. et al. Enrichment of Uncommon Bacteria in Soil by Fractionation Using a Metal Mesh Device. ANAL. SCI. 37, 1295–1300 (2021). https://doi.org/10.2116/analsci.21P042

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  • DOI: https://doi.org/10.2116/analsci.21P042

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