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Identification of antiarrhythmic drugs and their metabolites in urine

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Abstract

Identification of the antiarrhythmic drugs ajmaline, aprindine, diltiazem, disopyramide, flecainide, gallopamil, lidocaine, lorcainide, mexiletine, phenytoin, prajmaline, propafenone, quinidine, sparteine, tocainide and verapamil and their metabolites in urine is described. After acid hydrolysis of the conjugates, extraction and acetylation, the urine samples were analysed by computerized gas chromatography-mass spectrometry. Using ion chromatography with the selective ions m/z 58, 72, 84, 86, 136, 224, 266, and 426, the possible presence of antiarrhythmic drugs and/or their metabolites was indicated. The identity of positive signals in the reconstructed ion chromatograms was confirmed by a visual or computerized comparison of the stored full mass spectra with the reference spectra. The ion chromatograms, reference mass spectra and gas Chromatographic retention indices (OV-101) are documented. The method presented is integrated in a general screening procedure (general unknown analysis) for several groups of drugs.

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Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie der Universität des Saarlandes, D-6650, Homburg/Saar, Federal Republic of Germany

    Hans H. Maurer

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  1. Hans H. Maurer
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Additional information

Dedicated to Professor Dr med. Ilmar Jurna, Homburg (Saar), on the occasion of his 60th birthday. Some of these results were reported at the congress “Biochemische Analytik '88” of the German Society of Clinical Chemistry in München (FRG), April 19–22 1988 (Maurer and Pfleger 1988 a) and at the annual meeting of the German Pharmaceutical Society in Erlangen (FRG), September 7–10 1988 (Maurer 1988 a).

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Maurer, H.H. Identification of antiarrhythmic drugs and their metabolites in urine. Arch Toxicol 64, 218–230 (1990). https://doi.org/10.1007/BF02010728

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  • DOI: https://doi.org/10.1007/BF02010728

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